(1) Following central retinal artery ligation, infarction of the retinal ganglion cells was reflected by a 97 per cent reduction in the radioactively labeled protein within the optic nerve.
(2) Because cystine in medium was converted rapidly to cysteine and cysteinyl-NAC in the presence of NAC and given that cysteine has a higher affinity for uptake by EC than cystine, we conclude that the enhanced uptake of radioactivity was in the form of cysteine and at least part of the stimulatory effect of NAC on EC glutathione was due to a formation of cysteine by a mixed disulfide reaction of NAC with cystine similar to that previously reported for Chinese hamster ovarian cells (R. D. Issels et al.
(3) Estimations of the degree of incorporation of 14C from the radioactive labeled carbohydrate into the glycerol and fatty acid moieties were carried out.
(4) Most of the radioactivity in spleen cells from these rats were associated with antigen-reactive cells which formed rosettes specifically with HO erythrocytes.
(5) Q In radioactive decay, different materials decay at different rates, giving different half lives.
(6) Blood flow was measured in leg and torso skin of conscious or anesthetized sheep by using 15-micron radioactive microspheres (Qm) and the 133Xe washout method (QXe).
(7) During the digestion of these radiolabeled bacteria, murine bone marrow macrophages produced low-molecular-weight substances that coeluted chromatographically with the radioactive cell wall marker.
(8) In oleate-labeled particles, besides phosphatidic acid the product of PLD action radioactivity was also detected in diglyceride as a result of resident phosphatidate phosphohydrolase, which hydrolyzed the phosphatidic acid.
(9) Control incubations revealed an inherent difference between the two substrates; gram-positive supernatants consistently contained 5% radioactivity, whereas even at 0 h, those from the gram-negative mutant released 22%.
(10) The remainder of the radioactivity appeared chromatographically just prior to the bisantrene peak, indicating that compounds more polar than the parent were present as transformation products.
(11) High radioactivities were observed in the digestive organs, mesenteric lymphnodes, liver, pancreas, urinary bladder, fat tissue, kidney and spleen after oral administration to rats.
(12) When labelled long-chain fatty acids or glycerol were infused into the lactating goat, there was extensive transfer of radioactivity into milk in spite of the absence of net uptake of substrate by the mammary gland.
(13) Treatment with trypsin gave essentially one radioactive peptide, the active site peptide, of approximately 2300 molecular weight.
(14) Radioactivity attained in different tissues at different times after a single intraperitoneal injection of 3H-gentamicin into male rats was determined using scintillation counting.
(15) The present in vitro studies show that it is found as beta-endorphin in bovine pituitary slices incubated with radioactive amino acid precursor [35S]methionine.
(16) Bacterial adherence to vascular sutures was evaluated in vitro using radioactively labeled Staphylococcus aureus.
(17) Under the same conditions, no radioactive estrogen could be identified in adrenal and placental incubations.
(18) Four hours after injection radioactivity was identified in the spleen, liver, and bone marrow.
(19) Chromatographic analysis of this radioactivity reveals that the octadecapeptide gives rise to much higher tissue levels of intact peptide and we believe that this acts as a depot and gives rise to the sustained blood concentrations and prolonged biological effects observed with this peptide.
(20) 25% of the incorporated radioactivity in protoplast lysates and approx.
Tritium
Definition:
Example Sentences:
(1) Biliary and urinary excretion of five tritium-labelled cardiac glycosides, i.e.
(2) Tritium-labeled ribonucleic acid precursors, including cytidine, uridine, and orotic acid, were injected into rats with dated pregnancies (14 to 21 days) and virgin rats.
(3) Ovarian aromatase activity, assessed by incubating the homogenised ovaries of treated rats with tritium-labelled androstenedione (0.2 microM) or testosterone (1 microM), indicated that residual enzyme activity was reduced compared with controls.
(4) The adenylate cyclase activator forskolin as well as 8-bromo-cyclic AMP enhanced the electrically evoked release of 3H-noradrenaline and 3H-5-hydroxytryptamine from superfused rat neocortical slices and that of 3H-dopamine from neostriatal slices with comparable EC50's of about 0.5 and 50 microM, respectively, without affecting spontaneous tritium efflux.
(5) It was also found that tritium release also occurred, although to a lesser extent, when tissues were fixed in glutaraldehyde or osmium tetroxide using collidine as a buffer, and this release was not significant when collidine was applied to previously fixed tissues.
(6) The standardised mortality ratios were 889 (six deaths) in employees monitored for contamination by tritium, 254 (nine deaths) in those monitored for contamination by other radionuclides, and 385 (nine deaths) in those with dosimeter readings totalling more than 50 mSv (5 rem); but the same nine subjects tended to account for each of these significantly raised ratios.
(7) 3T3 cells were also used for the incorporation of tritium-thymidine.
(8) Isolated hepatocytes carry out the N-demethylation of dansylamide at near linear rates for up to 8 h. This reaction was measured by following the release of tritium into water on hydroxylation of 3H-labeled methyl groups.
(9) Similar low deuterium and tritium isotope effects were measured with a purified rat liver cytochrome P-450 preparation and horseradish peroxidase.
(10) The effects of alpha-adrenoceptor agonists and antagonists on the electrically (slices) or potassium-evoked (synaptosomes) tritium overflow were studied.
(11) During the monodirectional conversion of D-[2-3H]mannose 6-phosphate to D-fructose 6-phosphate and D-fructose 1,6-bisphosphate, the reaction velocity is one order of magnitude lower than with D-[U-14C]mannose 6-phosphate and little tritium (less than 6%) is transferred intramolecularly.
(12) The 72-hour cumulative urinary excretion of total tritium was 28.2% of the administered dose and only 38.3% of this resided in unchanged HHT.
(13) However, higher doses of diltiazem (60 microM) reduced the tritium overflow elicited by RNS or Vt but enhanced that caused by KCl.
(14) The substrate was labelled by the catalytic addition of tritium to cerebroside sulphate.
(15) In contrast, in synaptosomes superfused with Ca(2+)-free Krebs-Henseleit solution containing 15 mM K+ throughout, ethanol did not affect the tritium overflow evoked by 2 min introduction of 75 microM Ca2+ into the superfusion fluid.
(16) We have assessed a multiwire camera for the high speed, quantitative autoradiography of tritium-labelled substances in two-dimensional systems.
(17) Dipyridamole (5 x 10(-5) M) and erythro-9-2-hydroxy-3 nonyl adenosine (EHNA) (10(-4) M) also reduced tritium release.
(18) Tritium thymidine incorporation rates inform about DNA-synthesis.
(19) GR43175 (1 x 10(-8) to 1 x 10(-5) M) inhibited the electrically evoked release of tritium from renal noradrenergic nerves but did not affect the basal (non-stimulated) release of tritium or perfusion pressure.
(20) The activity of aromatase in microsomal preparations was assayed by determining the rate of incorporation of tritium from 1-[3H]androstenedione into [3H]water.