(1) Refolding was observed by injection of denatured protein into columns having isocratic concentrations in the transition and native base-line zones.
(2) Both enzymes are capable of catalyzing the refolding of thermally denatured type III collagen.
(3) Air-regenerated monomers of bovine seminal ribonuclease have been found capable of reassociating into native dimers, whereas monomers refolded in the presence of a glutathione redox mixture do not reassociate into dimers [Smith, K. G., D'Alessio, G. and Schaffer, S. W. (1978) Biochemistry 17, 2633-2638].
(4) When samples at pH 3.5 were dialyzed against 0.1 M ammonium acetate (pH 6.9) to refold interferon gamma, the samples that contained NaCl in acid formed aggregates upon dialysis while those without NaCl formed a dimer apparently identical with the starting protein (i.e., before acid treatment).
(5) When refolding is performed in the presence of methotrexate, an analogue of dihydrofolate, and NADPH, NADPH binds, as determined from changes in NADPH fluorescence, to the third observed intermediate rather than the last (fourth) species formed.
(6) In contrast, import of the surface-bound unfolded precursor requires ATP, but no potential; it is accompanied by a refolding inside the mitochondria.
(7) In the present study, the reversibility of the unfolding-refolding process induced by guanidine hydrochloride was investigated for the intact protein and the isolated domains.
(8) Convenient and rapid assays for detecting native protein are critical for developing a refolding procedure.
(9) In vitro refolding of the urea-unfolded, monomeric, mitochondrial enzyme rhodanese (thiosulfate sulfur-transferase; EC 2.8.1.1) is facilitated by the chaperonin proteins cpn60 and cpn10 from Escherichia coli at 37 degrees C, but the refolding is strongly inhibited at 10 degrees C. In contrast, the unassisted refolding of rhodanese is efficient at 10 degrees C, but the refolding efficiency decreases as the temperature is raised.
(10) Furthermore, the necessity to reactivate proteins at low protein concentrations due to its tendency to aggregate at high concentrations was overcome by a step-by-step addition of denatured and reduced protein into the refolding solution.
(11) (1990) Biochemistry (preceding paper in this issue)] were used to propose kinetic models for the unfolding and refolding of ribonuclease T1.
(12) The fractional renaturation of rhodanese due to the fast phase, monitored in various concentrations of GdmCl, describes a transition centered at 3.5 M GdmCl which is very similar to the higher of the two transitions observed in the reversible refolding.
(13) At dilute protein concentrations the refolding can be studied independent of the association phenomena.
(14) The effect of protein concentration and denaturant concentration on the kinetics of refolding were studied.
(15) Thus, the absence of a covalent linkage between the two proteolytic fragments of the enzyme molecule apparently does not affect the refolding.
(16) Downstream processing of the fusion proteins involved isolation of inclusion bodies, cleavage at the Asn-Gly bond, refolding of the reduced IGF-I peptide and purification to homogeneity.
(17) It appeared that the virus protein coat was sufficiently plastic so that the initial conformational change resulting from the alteration of an arginine residue (to possibly an ornithine residue) was at least partially reversible and that the virus tail proteins then refolded to produce a stable and active virus particle.
(18) This approach was found to be effective in minimizing the refolding difficulties and allowed accessibility to the synthesis of analogues in this class of compounds.
(19) At high temperatures above 60 degrees C, where the native enzymes are stable but their spontaneous refoldings upon dilution of guanidine HCl fail, the chaperonin induces productive refolding in an ATP-dependent manner.
(20) Over a period of several hours, refolded and inactive prorenin not bound to the column slowly regains 5% to 10% renin activity, even when maintained under conditions that are optimal for zymogen inactivation.
Remold
Definition:
(v. t.) Alt. of Remould
Example Sentences:
(1) The patient is allowed to do functional exercises 24 hours after reduction with the aid of the spring stepping roller, which not only helps dissipate swelling in the early stage but also remold the articular facet.
(2) The forward slipping occurs as a result of forward remolding of the z-a joints secondary to localized degenerative arthritis of these joints.
(3) The histological structure of the glomeruli returned almost to normal 15 weeks after the venom injection, with occasional features of remolded-healing, although a small number of glomeruli still showed persisted mild segmental mesangial proliferation as well as mild increase of PAM-positive substance in the mesangial area.
(4) Then we take a look at how hospitals' technology acquisition strategies--and vendors' research and development plans--are being remolded by the new capital regulation.
(5) Where gross deformity has existed, the use of an "oyster splint" seems to act as a pressure remolding device.
(6) In addition, thermoplastic splints are lightweight, less bulky, more durable, water-resistant, and easily remolded.
(7) Personally, I do not feel that any biological lens, be it a cornea which has had incisions as with radial keratotomy, or a cornea which is remolded in one way or another as with the excimer laser, will produce predictable refractive results to the extent that is required to satisfy the general needs of the myopic population.
(8) A lack of introns has previously been observed for the human leukosialin gene (Shelley, C. S., Remold-O'Donnell, E., Rosen, F. S. and Whitehead, A. S., Biochem.
(9) His answer was remoldable craniomandibular appliances.
(10) These results suggest that TRAP is useful as a marker of bone remolding in children.
(11) Second, we extend the model by allowing a surviving polymer to act upon--to "remold"--its environment; the nature of the environmental action is governed by the "molding" matrix M. When the mold M is the identity matrix, the feedback algorithm reduces to a Hebb learning algorithm form, and a surviving polymer acts to enhance its own survival prospects.
(12) Since considerable remolding of brain structures (e.g., cell death and modifications of neuronal architecture) occurs during development, we ask if these cells are preserved in the adult zebrafish and the extent to which neuronal morphology of the larva is conserved during ontogeny.
(13) The development of a remoldable craniomandibular (RCM) appliance is the result of four years of clinical research.
(14) Generally speaking, we must use all the tools in our armamentarium, including radiation, intra-keloid steroids, surgery and postoperative constant wound pressure in an effort to remold the newly forming collagen.
(15) It could be easily molded and remolded to fit the foot, as well as to adjust the position of the foot and ankle.
(16) Individually fitted plastic helmets, similar in style to football helmets, have been successfully used to remold the deformed heads of four infants.
(17) This report quantitatively compares long-term head remolding achieved by two methods for retarding bone reunion after midline craniectomy for sagittal suture synostosis.
(18) The remoldable craniomandibular appliance is designed as an inexpensive and time efficient method of arriving at a differential diagnosis for some patients with temporomandibular joint related headache.
(19) IL-4 abrogates the IFN-gamma-mediated activation of peripheral blood monocytes (M. Lehn, W. Y. Weiser, S. E. Engelhorn, S. Gillis, and H. G. Remold, 1989, J. Immunol.