What's the difference between scission and separation?
Scission
Definition:
(n.) The act of dividing with an instrument having a sharp edge.
Example Sentences:
(1) The density increase can be explained by post-crystallization, which is the result of oxidative chain scission.
(2) Activation of GV by monochromatic 450-nm radiation causes two specific DNA changes in human P3 cells in culture as shown by alkaline elution techniques: single-strand breaks (i.e., alkali-labile sites plus frank strand scissions) and DNA-to-protein covalent bond crosslinks.
(3) The results of cellular experiments using gene transfer frequencies as a measure of DNA rejoining strongly suggested that the A-T cell line had a greatly elevated frequency of misrepair of double-stranded DNA scissions.
(4) Four bisheterosteroids 3,17-dioxa-5 alpha-androstane, 3-thia-17-oxa-5 alpha-androstane, 3-aza-17-oxa-5 alpha-androstane, and 3-selena-17-oxa-5 alpha-androstane) were synthesized from 17-oxa-5 alpha-androstan-3 beta-ol via 5, 8, 8, and 9 steps, respectively, involving a second regioselective beta-scission of an alkoxyl radical as the key step.
(5) Complex formation does not result in DNA strand scission and studies of the chemical sensitivity of the complex suggest that the TFIIIA-DNA linkage may be through a phosphoramidate bond.
(6) It is concluded that Fragment B comprises the complementary portion of the heavy chain (remaining after scission of the polypeptide bond(s) releasing Fragment C) linked to the light chain by a disulfide bond.
(7) The technique involves strand scission or chemical base modification at structurally perturbed sites, replication arrest in a double-strand DNA sequencing reaction, and size analysis of replication products by electrophoresis on sequencing gels.
(8) Of particular interest, the alcohol-inducible form of liver microsomal cytochrome P-450 form 3a (P-450 IIE1) is the most active of the isozymes examined in the reductive beta-scission of the 13-hydroperoxide derived from linoleic acid and the 15-hydroperoxide derived from arachidonic acid as well as the model compounds cumyl hydroperoxide (alpha, alpha-dimethylbenzyl hydroperoxide) and t-butyl hydroperoxide.
(9) Free radicals induced by laser radiation are found to participate in DNA sugar-phosphate chain scission.
(10) In contrast, mutations which disrupted a conserved Asn-Pro-Gly-Pro (NPGP) sequence abolished primary scission.
(11) The occurrence of these latter forms indicates that double-strand scissions produced by bleomycin reaction consist of two single-strand scissions which are physically staggered on the complementary strands.
(12) In addition to representing one of the smallest and simplest ribozymes possible, strand scission occurs readily under physiological conditions, is unaffected by the presence of Mg2+, and displays salt, pH, and temperature optima of potential use in exploiting Mn2+ as a regulatory switch in intact cells.
(13) We report here that the DNA strand scission by dynemicin A is not only sequence-specific but also conformation-specific.
(14) Covalent closure by Escherichia coli ligase of a circular DNA containing single-chain scissions, when carried out in the presence of a combination of the DNA gyrase components alpha and beta, gives a positively supercoiled DNA upon removal of the bound protein molecules.
(15) One mole of DNPS is liberated per mole of free thiol in alpha 2M, consistent with cyanylation of the thiol liberated upon scission of the internal thiol esters by methylamine.
(16) The stability of the adducts was compared by analyzing the oligonucleotide for strand scission or depurination by polyacrylamide gel electrophoresis following treatment under either neutral, acidic or basic conditions or incubation in the presence of piperidine.
(17) The boron derivatives also caused L-1210 DNA strand scission.
(18) These data suggest that MCR works directly on cell nuclei and strand scission of DNA is one of the more important actions of the drug.
(19) Substitution of BrdU for single thymidine positions in a synthetic 40-base pair operator provides substrate for ultraviolet irradiation; upon irradiation, strand scission occurs at the BrdU residues.
(20) We show that the same distribution of supranucleosomal structures (even those containing internal DNA scissions) can be reconstituted from unfolded nuclear chromatin extracts as present in 10 mM or 600 mM NaCl.
Separation
Definition:
(n.) The act of separating, or the state of being separated, or separate.
(n.) Chemical analysis.
(n.) Divorce.
(n.) The operation of removing water from steam.
Example Sentences:
(1) It was tested for recovery and separation from other selenium moieties present in urine using both in vivo-labeled rat urine and human urine spiked with unlabeled TMSe.
(2) Chapter one Announcement of the Islamic Caliphate The announcement of the renewal of the caliphate in Iraq in the year 1427AH [2006] was the arbiter between division and separation as well as the glory of the Muslims.
(3) The adjacent gauge was separated from the ischemic segment by one large nonoccluded diagonal branch of the left anterior descending artery.
(4) The previous year, he claimed £1,415 for two new sofas, made two separate claims of £230 and £108 for new bed linen, charged £86 for a new kettle and kitchen utensils and made two separate claims, of £65 and £186, for replacement glasses and crockery.
(5) And this is the supply of 30% of the state’s fresh water.” To conduct the survey, the state’s water agency dispatches researchers to measure the level of snow manually at 250 separate sites in the Sierra Nevada, Rizzardo said.
(6) Completeness of isolation of the coronary and systemic circulations was shown by the marked difference in appearance times between the reflex hypotensive responses from catecholamine injections into the isolated coronary circulation and the direct hypertensive response from a similar injection when the circulations were connected as well as by the marked difference between the pressure pulses recorded simultaneously on both sides of the aortic balloon separating the two circulations.4.
(7) In the present study, respirometric quotients, the ratio of oral air volume expended to total volume expended, were obtained using separate but simultaneous productions of oral and nasal airflow.
(8) The sensitivity of an indirect fluorescent antibody (IFA) test (screening test) for the detection of antibodies to cytomegalovirus (CMV) was examined by using 128 serum specimens and quaternary aminoethyl (QAE)-Sephadex A50 column chromatography to separate IgM from IgG class antibodies.
(9) This experimental system allows separation of three B lymphocyte developmental stages: early differentiation in vitro, progression to IgM secretion in vivo, and late differentiation dependent upon mature T lymphocytes in vivo.
(10) The individual classes of drugs are first treated separately to highlight specific aspects of their quantification, and this is followed by an overview of those methods permitting the concomitant analysis of two or more antiepileptic compounds.
(11) The ADAM derivative of carnitine was separated from decomposition products of the reagent and related compounds such as amino acid derivatives on a silica gel column eluted with methanol-5% aqueous SDS-phosphoric acid (990:10:1).
(12) Each patient contributed only once to each phase (105 in phase 1, 107 in phase 2), but some entered both phases on separate occasions.
(13) Twenty volunteers were used for the measurement of pedal pressures for 15 trials during three separate sessions.
(14) The relative strength of the progressions varies with excitation wavelength and this, together with the absence of a common origin, indicates the existence of two independent emitting states with 0-0' levels separated by either 300 or 1000 cm-1.
(15) Densitometric analysis of myofibrillar proteins separated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that troponin I and troponin T were degraded during 60 minutes of CGI.
(16) In a separate exclusive interview , Alexis Tsipras, the increasingly powerful 37-year-old Greek politician now regarded by many as holding the future of the euro in his hands, told the Guardian that he was determined "to stop the experiment" with austerity policies imposed by Germany.
(17) Sephadex LH-20 column chromatography was used for the separation of the steroid prior to assay.
(18) The deletions and substitutions appear to occur in separate molecules.
(19) The canine system allows quantitative separation of native heme containing alpha and beta chains which recombine to for tetrameric hemoglobin with normal functional properties (n = 2.17).
(20) Prothrombin isolation on DEAE Sephadex failed to separate the abnormal population (prothrombin Clamart) from the normal one.