(1) The amino acid sequence deduced from the DNA sequence was determined for the plasmid-coded and the ScrY porin coded in the chromosome of Klebsiella pneumoniae.
(2) Primer extension analysis and site-directed mutagenesis were used to identify the precise location of the promoter of scrY, scrA, and scrB.
(3) This increase in sucrose permeability provided strong evidence that the ScrY protein functions as a sucrose porin.
(4) A putative cyclic AMP receptor protein binding site centered 72.5 bp upstream of the start point of transcription of scrY appeared to be essential for full activity of the scrY promoter.
(5) Furthermore, the presence of ScrY restored growth on maltodextrins in cells devoid of LamB, thus complementing the lack of this maltoporin.
(6) Reconstitution experiments with lipid bilayer membrane demonstrated that ScrY formed ion-permeable channels with properties very similar to those of general diffusion pores of enteric bacteria.
(7) This sounds shocking, but dig a little deeper and some of this soul-scrying voodoo becomes slightly less terrifying.
(8) The binding of different sugars to ScrY and LamB of E. coli is discussed with respect to the kinetics of sugar movement through the channel.
(9) A frameshift mutation in the scrY gene resulted in a dramatic decrease in sucrose transport with no effect on in vitro phosphorylation activity associated with enzyme IISer.
(10) There was 23% amino acid sequence identity between the ScrY protein and LamB, a maltose porin from Escherichia coli.
(11) One of the different gene products of the plasmid is the outer membrane protein, ScrY.
(12) During the molecular analysis of a plasmid-coded sucrose metabolic pathway of enteric bacteria, a gene, scrY, was found whose product, ScrY, had all the properties of a bacterial porin (Schmid et al., 1988).
(13) In ScrR+ cells, readthrough transcription from the putative scrK promoter into scrY accounted for less than 10% of scrY expression.
(14) The scrY gene, part of the pUR400-borne sucrose regulon, appeared to be transcribed from its own promoter, with the transcriptional start site located 58 bp upstream from the initiation codon.
(15) The four genes form an scr operon (gene order, scrK scrY scrA scrB, transcription from K to B), regulated by a repressor (gene scrR, 37 kD) and inducible by sucrose, fructose and fructose-containing oligosaccharides.
(16) Gene scrK apparently codes for an intracellular and ATP-dependent fructokinase (39 kD), while scrY seems to code for a sucrose porin (58 kD) in the outer cell membrane.
(17) The rate of diffusion of sucrose was 96 times greater than the rate of diffusion of lactose or maltose in liposomes containing the ScrY protein.
See
Definition:
(n.) A seat; a site; a place where sovereign power is exercised.
(n.) Specifically: (a) The seat of episcopal power; a diocese; the jurisdiction of a bishop; as, the see of New York. (b) The seat of an archibishop; a province or jurisdiction of an archibishop; as, an archiepiscopal see. (c) The seat, place, or office of the pope, or Roman pontiff; as, the papal see. (d) The pope or his court at Rome; as, to appeal to the see of Rome.
(v. t.) To perceive by the eye; to have knowledge of the existence and apparent qualities of by the organs of sight; to behold; to descry; to view.
(v. t.) To perceive by mental vision; to form an idea or conception of; to note with the mind; to observe; to discern; to distinguish; to understand; to comprehend; to ascertain.
(v. t.) To follow with the eyes, or as with the eyes; to watch; to regard attentivelly; to look after.
(v. t.) To have an interview with; especially, to make a call upon; to visit; as, to go to see a friend.
(v. t.) To fall in with; to have intercourse or communication with; hence, to have knowledge or experience of; as, to see military service.
(v. t.) To accompany in person; to escort; to wait upon; as, to see one home; to see one aboard the cars.
(v. i.) To have the power of sight, or of perceiving by the proper organs; to possess or employ the sense of vision; as, he sees distinctly.
(v. i.) Figuratively: To have intellectual apprehension; to perceive; to know; to understand; to discern; -- often followed by a preposition, as through, or into.
(v. i.) To be attentive; to take care; to give heed; -- generally with to; as, to see to the house.
Example Sentences:
(1) I can't wait to see what Christie and her patriarchy-smashing pals do next.
(2) You can see where the religious meme sprung from: when the world was an inexplicable and scary place, a belief in the supernatural was both comforting and socially adhesive.
(3) The sound of the ambulance frightened us, especially us children, and panic gripped the entire community: people believe that whoever is taken into the ambulance to the hospital will die – you so often don’t see them again.
(4) It would be fascinating to see if greater local government involvement in running the NHS in places such as Manchester leads over the longer term to a noticeable difference in the financial outlook.
(5) My boyfriend and I have been seeing each other for two years.
(6) The PUP founder made the comments at a voters’ forum and press conference during an open day held at his Palmer Coolum Resort, where he invited the electorate to see his giant robotic dinosaur park, memorabilia including his car collection and a concert by Dean Vegas, an Elvis impersonator.
(7) I can see you use humour as a defence mechanism, so in return I could just tell you that if he's massively rich or famous and you've decided you'll put up with it to please him, you'll eventually discover it's not worth it.
(8) What we’re doing is designed to improve people’s lives.” "I don't see race, colour or creed, and neither do my children," he added.
(9) But both for malaria and Aids we’re seeing the tools that will let us do 95-100% reduction.
(10) We investigated the incidence of skin cancer among patients who received high doses of PUVA to see whether such incidence increased.
(11) 'The French see it as an open and shut case,' says a Paris-based diplomat.
(12) If Deckard cannot see himself in the other, Roy can.
(13) We are pleased to see the process moving forward and look forward to its resolution,” a Target spokeswoman, Molly Snyder, said in an emailed statement.
(14) Swedes tend to see generous shared parental leave as good for the economy, since it prevents the nation's investment in women's education and expertise from going to waste.
(15) The association constants K'A, KN, and K'N in the scheme (see article), were determined for the magnesium salts of ADP, adenyl-5'-yl imidodiphosphate AMP-P(NH)P, and PPi.
(16) The IgG index (formula: see text) corrects for the influence of serum protein abnormalities as well as a bloodbrain barrier damage and is, therefore, a better measure for the presence of an IgG elevation in CSF due to IgG synthesis, when compared with other IgG quotients commonly used.
(17) Stimuli presented to this island could be detected and discriminated, although the subject reported he did not see them.
(18) However, as all subjects had normal hearing and maximum speech discrimination scores pre-smoking, it can only be concluded that smoking marihuana did not worsen the hearing--the experiments were not designed to see whether it would improve hearing.
(19) A Palestinian delegation was to hold truce talks on Sunday in Cairo with senior US and Egyptian officials, but Israel has said it sees no point in sending its negotiators to the meeting, citing what it says are Hamas breaches of previous agreed truces.
(20) We conclude by looking at several specific models and seeing how the results relate to previous work.