(1) The amino acid sequence deduced from the DNA sequence was determined for the plasmid-coded and the ScrY porin coded in the chromosome of Klebsiella pneumoniae.
(2) Primer extension analysis and site-directed mutagenesis were used to identify the precise location of the promoter of scrY, scrA, and scrB.
(3) This increase in sucrose permeability provided strong evidence that the ScrY protein functions as a sucrose porin.
(4) A putative cyclic AMP receptor protein binding site centered 72.5 bp upstream of the start point of transcription of scrY appeared to be essential for full activity of the scrY promoter.
(5) Furthermore, the presence of ScrY restored growth on maltodextrins in cells devoid of LamB, thus complementing the lack of this maltoporin.
(6) Reconstitution experiments with lipid bilayer membrane demonstrated that ScrY formed ion-permeable channels with properties very similar to those of general diffusion pores of enteric bacteria.
(7) This sounds shocking, but dig a little deeper and some of this soul-scrying voodoo becomes slightly less terrifying.
(8) The binding of different sugars to ScrY and LamB of E. coli is discussed with respect to the kinetics of sugar movement through the channel.
(9) A frameshift mutation in the scrY gene resulted in a dramatic decrease in sucrose transport with no effect on in vitro phosphorylation activity associated with enzyme IISer.
(10) There was 23% amino acid sequence identity between the ScrY protein and LamB, a maltose porin from Escherichia coli.
(11) One of the different gene products of the plasmid is the outer membrane protein, ScrY.
(12) During the molecular analysis of a plasmid-coded sucrose metabolic pathway of enteric bacteria, a gene, scrY, was found whose product, ScrY, had all the properties of a bacterial porin (Schmid et al., 1988).
(13) In ScrR+ cells, readthrough transcription from the putative scrK promoter into scrY accounted for less than 10% of scrY expression.
(14) The scrY gene, part of the pUR400-borne sucrose regulon, appeared to be transcribed from its own promoter, with the transcriptional start site located 58 bp upstream from the initiation codon.
(15) The four genes form an scr operon (gene order, scrK scrY scrA scrB, transcription from K to B), regulated by a repressor (gene scrR, 37 kD) and inducible by sucrose, fructose and fructose-containing oligosaccharides.
(16) Gene scrK apparently codes for an intracellular and ATP-dependent fructokinase (39 kD), while scrY seems to code for a sucrose porin (58 kD) in the outer cell membrane.
(17) The rate of diffusion of sucrose was 96 times greater than the rate of diffusion of lactose or maltose in liposomes containing the ScrY protein.
Shout
Definition:
(v. i.) To utter a sudden and loud outcry, as in joy, triumph, or exultation, or to attract attention, to animate soldiers, etc.
(v. t.) To utter with a shout; to cry; -- sometimes with out; as, to shout, or to shout out, a man's name.
(v. t.) To treat with shouts or clamor.
(n.) A loud burst of voice or voices; a vehement and sudden outcry, especially of a multitudes expressing joy, triumph, exultation, or animated courage.
Example Sentences:
(1) David Cameron was accused of revealing his ill-suppressed Bullingdon Club instincts when he shouted at the Labour frontbencher Angela Eagle to "calm down, dear" as she berated him for misleading MPs at prime minister's questions.
(2) They shouted at her: ‘Keep your hands in the air!’ They told her: ‘We’re going to shoot.’ “The shooting resumed.
(3) It’s around this point in the film’s chronology that Rodman makes his now infamous appearance on CNN , where he rejected calls to assist in the release of American prisoner Kenneth Bae and shouted at interviewer Chris Cuomo.
(4) He encountered one couple en route to the MSPs’ meeting, who said “Glad you could visit, Jeremy,” and “Well done!” And outside a nearby cafe, a man cradling his baby daughter in the sunshine shouted out to him: “Thanks for bringing humanity back to politics.
(5) North Korea's blustering defiance at the annual US-South Korean exercises masks just a little fear that they could easily be turned into an all-out attack, and seems to work on the principle that the more you shout, the safer you will be.
(6) Four University of the Free State students filmed themselves drinking in a bar and then one of them urinating into a stew before feeding it to five black staff members, four of them women, at their dormitory on the Bloemfontein campus accompanied by shouts of "take it, take it".
(7) We all knew from the beginning that Little Mix would be in with a shout for the final rounds, because they were young and possessed of more than a modicum of talent and so no one … old … no matter how talented, would pop their bubble.
(8) An excitable audience filled Glasgow's all-smoking, all-drinking Old Fruitmarket with shouted requests to Zevon who, at 53, looks a little mashed up by life.
(9) He shouted “Cops Lives Matter” before being drowned out with the “Bernie” chant.
(10) And when the international community shouts selectively about human rights it encourages conservatives to feel that they are being hectored again by “ Little Satan ” Britain or “Great Satan” America.
(11) The defendants punched their air with their fists and shouted "peacefully" as their sentences were handed down, according to relatives.
(12) When we had a morning practice session, and some players were a bit sluggish, he would call them out to the middle of the pitch and shout: ‘Dilly-ding, dilly-dong!’ When I read this story about Leicester, I just started laughing because all those funny moments with him came rushing back into my head.” That Ranieri has a sense of humour is hardly new information.
(13) A Chelsea fan filmed while racist chants were shouted on the Paris Métro was a “vocal” supporter of Ukip, even posing with the party’s leader, Nigel Farage.
(14) Does this count as campaigning?” “When was the last time you flipped a steak?” “What does it feel like to be in Iowa?” “Can you bring the reporters some meat?” “Are you running, Hillary,” one reporter shouted, finally, “from us?” Then Bill and Hillary disappeared around the corner; three quarters of the media scrum vanished, deflated.
(15) Early in the unrest protesters carried crosses and shouted anti-sectarian slogans: "Muslims, Christians, Alawis are all one."
(16) The women in Wednesday's protest climbed up on the gates of the justice ministry until police pulled them down and hustled them shouting into the building as an angry crowd gathered, many of them lawyers there for work.
(17) "25 at 4 [2,500 shares at 400p each], print that quickly," shouts one trader.
(18) Up went the shouts for a second penalty, Koller ran along the touchline to add his voice, but the referee said no.
(19) An officer claimed McKenna had shouted: "Fucking Yankee bastards out."
(20) In fact, I think I heard "it's not rape if you shout 'surprise'", at least 20 years ago and it hasn't aged well.