(1) The amino acid sequence deduced from the DNA sequence was determined for the plasmid-coded and the ScrY porin coded in the chromosome of Klebsiella pneumoniae.
(2) Primer extension analysis and site-directed mutagenesis were used to identify the precise location of the promoter of scrY, scrA, and scrB.
(3) This increase in sucrose permeability provided strong evidence that the ScrY protein functions as a sucrose porin.
(4) A putative cyclic AMP receptor protein binding site centered 72.5 bp upstream of the start point of transcription of scrY appeared to be essential for full activity of the scrY promoter.
(5) Furthermore, the presence of ScrY restored growth on maltodextrins in cells devoid of LamB, thus complementing the lack of this maltoporin.
(6) Reconstitution experiments with lipid bilayer membrane demonstrated that ScrY formed ion-permeable channels with properties very similar to those of general diffusion pores of enteric bacteria.
(7) This sounds shocking, but dig a little deeper and some of this soul-scrying voodoo becomes slightly less terrifying.
(8) The binding of different sugars to ScrY and LamB of E. coli is discussed with respect to the kinetics of sugar movement through the channel.
(9) A frameshift mutation in the scrY gene resulted in a dramatic decrease in sucrose transport with no effect on in vitro phosphorylation activity associated with enzyme IISer.
(10) There was 23% amino acid sequence identity between the ScrY protein and LamB, a maltose porin from Escherichia coli.
(11) One of the different gene products of the plasmid is the outer membrane protein, ScrY.
(12) During the molecular analysis of a plasmid-coded sucrose metabolic pathway of enteric bacteria, a gene, scrY, was found whose product, ScrY, had all the properties of a bacterial porin (Schmid et al., 1988).
(13) In ScrR+ cells, readthrough transcription from the putative scrK promoter into scrY accounted for less than 10% of scrY expression.
(14) The scrY gene, part of the pUR400-borne sucrose regulon, appeared to be transcribed from its own promoter, with the transcriptional start site located 58 bp upstream from the initiation codon.
(15) The four genes form an scr operon (gene order, scrK scrY scrA scrB, transcription from K to B), regulated by a repressor (gene scrR, 37 kD) and inducible by sucrose, fructose and fructose-containing oligosaccharides.
(16) Gene scrK apparently codes for an intracellular and ATP-dependent fructokinase (39 kD), while scrY seems to code for a sucrose porin (58 kD) in the outer cell membrane.
(17) The rate of diffusion of sucrose was 96 times greater than the rate of diffusion of lactose or maltose in liposomes containing the ScrY protein.
Sory
Definition:
(n.) Green vitriol, or some earth imregnated with it.
Example Sentences:
(1) On the eve of Charles Taylor's conviction for "aiding and abetting" such attacks as he and his allies sought control of lucrative diamond fields, Sorie maintained his silence.
(2) Sorie Sawanah, a former taxi driver, had his arm chopped off by drug-crazed child soldiers rampaging through Sierra Leone's capital, Freetown, in 1999.
(3) "I don't want to recall them days," Sorie said, covering his face with a shaking hand.
(4) Sorie Sawanah, reached by phone as dusk approached in Freetown, finally broke his silence.
(5) The risk factors for acute malnutrition retained after logistical regression are the age groups of 6-17 months and 18-29 months, the areas of Guidan Roumdji, Tahoua, Tajae, Guidan Sori and the group of children that have contracted diarrhea during the 15 days preceding the survey.
(6) Blinding caused involution of the testes and acces sory sex organs, and decreased pituitary prolactin levels.
(7) The formation of secondary sori in whorls of Polysphondylium pallidum provides an attractive model system for the study of symmetry breaking during morphogenesis.
(8) Sorie's son Ibrahim had nightmares for years about the scene he witnessed cowering behind a bush.
(9) Sory Diakite, the mayor of Konna, says the dead included children who drowned after they threw themselves into a river in an effort to escape the bombs.
(10) To be sure, it would be necessary to collect sori, clusters of sporangia, from sexually mature plants.
(11) Sorie Sawanah, a former taxi driver, rarely speaks about the day he became one of the statistics of the brutal "Operation No Living Thing", when drug-crazed child soldiers rampaged through Freetown in 1999.