(1) The amino acid sequence deduced from the DNA sequence was determined for the plasmid-coded and the ScrY porin coded in the chromosome of Klebsiella pneumoniae.
(2) Primer extension analysis and site-directed mutagenesis were used to identify the precise location of the promoter of scrY, scrA, and scrB.
(3) This increase in sucrose permeability provided strong evidence that the ScrY protein functions as a sucrose porin.
(4) A putative cyclic AMP receptor protein binding site centered 72.5 bp upstream of the start point of transcription of scrY appeared to be essential for full activity of the scrY promoter.
(5) Furthermore, the presence of ScrY restored growth on maltodextrins in cells devoid of LamB, thus complementing the lack of this maltoporin.
(6) Reconstitution experiments with lipid bilayer membrane demonstrated that ScrY formed ion-permeable channels with properties very similar to those of general diffusion pores of enteric bacteria.
(7) This sounds shocking, but dig a little deeper and some of this soul-scrying voodoo becomes slightly less terrifying.
(8) The binding of different sugars to ScrY and LamB of E. coli is discussed with respect to the kinetics of sugar movement through the channel.
(9) A frameshift mutation in the scrY gene resulted in a dramatic decrease in sucrose transport with no effect on in vitro phosphorylation activity associated with enzyme IISer.
(10) There was 23% amino acid sequence identity between the ScrY protein and LamB, a maltose porin from Escherichia coli.
(11) One of the different gene products of the plasmid is the outer membrane protein, ScrY.
(12) During the molecular analysis of a plasmid-coded sucrose metabolic pathway of enteric bacteria, a gene, scrY, was found whose product, ScrY, had all the properties of a bacterial porin (Schmid et al., 1988).
(13) In ScrR+ cells, readthrough transcription from the putative scrK promoter into scrY accounted for less than 10% of scrY expression.
(14) The scrY gene, part of the pUR400-borne sucrose regulon, appeared to be transcribed from its own promoter, with the transcriptional start site located 58 bp upstream from the initiation codon.
(15) The four genes form an scr operon (gene order, scrK scrY scrA scrB, transcription from K to B), regulated by a repressor (gene scrR, 37 kD) and inducible by sucrose, fructose and fructose-containing oligosaccharides.
(16) Gene scrK apparently codes for an intracellular and ATP-dependent fructokinase (39 kD), while scrY seems to code for a sucrose porin (58 kD) in the outer cell membrane.
(17) The rate of diffusion of sucrose was 96 times greater than the rate of diffusion of lactose or maltose in liposomes containing the ScrY protein.
Spry
Definition:
(superl.) Having great power of leaping or running; nimble; active.
Example Sentences:
(1) You’d think such a spry, successful man would busy himself with other things besides crawling into a pile of stuffed animals to scare his daughter’s date.
(2) Harry was brought into the room in a wheelchair - little and frail but, given his great age, astonishingly spry-looking.
(3) She has spry, bright eyes which match her curly blonde locks, and there’s a playful elegance in the vivid turquoise scarf and pink necklace she wears against her black outfit.
(4) But Winning’s got an attractively impish spirit and there are some spry jokes here.
(5) Matthew Spry is director at planning consultancy Nathaniel Lichfield & Partners Interested in housing?
(6) Spry little David is the last surviving grandson of John D. It was Granddad Rockefeller who famously declared competition a sin, and built one of the world's great fortunes.
(7) But governments are forever telling us that this global corporate entity is in fact an agile, mobile and spry creature; that companies will relocate, taking jobs and tax revenue with them rather than succumb to any legislation that will limit their ability to extract as much profit as possible.
(8) Recent evidence suggests that although the eosinophil does posses some regulatory capabilities, its presence is, in fact, a harbinger of tissue destruction (Gleich and Adolphoson, 1986, Wardlaw and Kay, 1987; Spry, 1988).
(9) The cases of sexual abuse by Catholic priests, Eunice Spry – a Jehovah's Witness who forced sticks down the throats of her foster children and made them eat their own vomit – or Khyra Ishaq, who was starved to death because her Muslim mother and stepfather believed she was possessed by an evil spirit, were all received in horror and condemned by their faith communities.
(10) My mother-in-law is a reasonably spry, mentally alert 87-year-old.
(11) Shanbag, a spry, watchful man in his mid-50s, smiles quietly when I ask.
(12) These proteins were designated sprI and sprII (small, proline rich).
(13) It would have been amusing to see Barry Bonds rise up as a spry shooting guard only to suddenly become a center after seeing how many commercials they were giving Shaq.
(14) The plan then is to be put through his paces by Roger Spry, a highly respected fitness and conditioning coach whom Bamford has employed at his own expense to get him in the best possible shape for a shot at the big time.
(15) Spry and alert at 89, Luis Iriondo Aurtenetxea sat down with me in the offices of Gernika Gogoratuz, which means "Remembering Gernika" in the Basque language.
(16) Chabrol's last two films, La Fille Coupée en Deux (A Girl Cut in Two, 2007) and Bellamy (2009), both mordant crime thrillers with a valedictory nod to Hitchcock, showed him to be as spry as ever.
(17) The new proteins were designated sprI and sprII (small, proline rich).
(18) His film is a spry, experimental mix of narrative trickery and visual intelligence, a self-referential noir, featuring sex, drugs, murder and a minor role for the excellent Kenneth Cranham as a London detective trying to sell a movie script.
(19) Good!” said a spry-looking Bill Clinton , wearing light blue pants and a dark shirt, after Obama made his putt on the first hole at Farm Neck golf club in Oak Bluffs.