(1) The results support the speculation that the product of SELD is a phosphoselenoate with the phosphate moiety derived phosphoselenoate from the gamma-phosphate group of ATP.
(2) The biological activities of the wild type and mutant proteins were studied using E. coli MB08 (selD-) transformed with plasmids containing the selD genes.
(3) Purified SELD protein is a monomer of 37 kDa in its native state and catalyses a selenium-dependent ATP-cleavage reaction delivering AMP and releasing the beta-phosphate as orthophosphate.
(4) Moreover, synthesis of enzymically active protein in a transformed E. coli selD mutant strain indicated that there is a nonspecific mechanism of selenocysteine incorporation.
(5) Transformation of the mutant Salmonella strain with a plasmid bearing the E. coli selD gene restored formate dehydrogenase activity, 75Se incorporation into formate dehydrogenase seleno-polypeptides and [75Se]seleno-tRNA synthesis.
(6) Mutation of a single gene, referred to as selA1 in Salmonella typhimurium and as selD in Escherichia coli, results in the inability of these organisms to insert selenium specifically into the selenopolypeptides of formate dehydrogenase and into the 2-selenouridine residues of tRNAs.
(7) The formation of selenocysteine depended on the presence of functional products of the selA and selD genes but not of the selB gene.
(8) 4) SELD however, was accompanied by the longer duration of surgery with more blood loss and by higher incidence of complications, than conventional R2, R3 dissection.
(9) This fact seems to warrant SELD for advanced gastric cancer.
(10) Complementation of the mutation in S. typhimurium with the selD gene from E. coli indicates functional identity of the selA1 and selD genes.
(11) For male Ss, verbal reinforcement increased self-disclosure relative to the interviewer seld-disclosure condition.
(12) In the absence of the complementary enzyme(s), the SELD protein catalyzes the synthesis of a labile selenium donor compound from selenide and ATP.
(13) The longer initiated just upstream of the orf183 gene, whereas the 5' end of the other mapped in a 116-bp nontranslated region between orf183 and selD.
(14) Four genes have been identified so far: selA and selB (at the fdhA locus), selC (previously fdhC), and selD (previously fdhB).
(15) It was precluded that any putative covalent or non-covalent ligand of SELD not removed during purification participated in the reaction.
(16) However, supplementation of the deficient enzyme preparation with the purified selD gene product (SELD protein) restored synthesis of seleno-tRNAs.
(17) Transformation with an additional plasmid carrying an E. coli formate dehydrogenase selenopolypeptide-lacZ gene fusion showed that the selD gene allowed readthrough of the UGA codon and synthesis of beta-galactosidase in the Salmonella mutant.
(18) The selD gene from Escherichia coli, whose product is involved in selenium metabolism, has been cloned and sequenced.
(19) Gene disruption experiments demonstrated that the SelD protein is required both for the incorporation of selenium into the modified nucleoside 5-methylaminomethyl-2-selenouridine of tRNA and for the biosynthesis of selenocysteine from an L-serine residue esterbonded to tRNA(Ser)(UCA).
(20) One hundred and sixty three cases of gastric cancer, treated with SELD is reviewed.
Sled
Definition:
(n.) A vehicle on runners, used for conveying loads over the snow or ice; -- in England called sledge.
(n.) A small, light vehicle with runners, used, mostly by young persons, for sliding on snow or ice.
(v. t.) To convey or transport on a sled; as, to sled wood or timber.
Example Sentences:
(1) The two completely different total knee-endoprostheses (hinge type and sled or runner type) have been compared concerning construction and ability for take up or transmission of forces and moments.
(2) In order to assess the effect of extravestibular gravity receptors on perception and control of body position against that of the otoliths, the subject (S) is exposed to gravitoinertial forces along the spinal (Z) axis on a tiltable board and on a sled centrifuge.
(3) Eighteen young male subjects with NAMRL sled test experience to 15 G in --Gx acceleration were measured for physical characteristics of the head and neck and general body anthropometry.
(4) A Teflon sled, Proplast malar implant and ptosis correction acheived the desired results.
(5) No difference in risk of injury was found regarding the type of sled used, the number of children, or their position on the sled or for those children with a history of prior sledding experience.
(6) A state law enforcement agency, SLED, has taken over the investigation into the shooting along with the Justice Department and FBI.
(7) Each year, the winning team takes a special trip with him: this year’s winners will go dog-sledding on a glacier in Iceland; when Reilly and the DeAngeluses won in 2012, their team spent a weekend in a Scottish castle.
(8) These burs were tested utilizing custom-built equipment consisting of a frictionless air sled to which the Macor substrate was attached.
(9) In February he will leave northern Canada to trek more than 1,000km to the North Pole; what's different this time is that he is travelling with two fellow polar explorers, his friends Ann Daniels and Martin Hartley, and they will be dragging with them not just food and repair kits but 100kg sleds each, laden with equipment to take up to 12m readings of the depth and density of snow and ice beneath their feet.
(10) Gross examination revealed that the sleds were secured in position until well encapsulated.
(11) Over 150 Navy enlisted men have been subjected to impact acceleration on a sled propelled by a nitrogen-powered horizontal accelerator.
(12) By a systematic analysis of the so called sled-prostheses is to be shown to differantiate between real sled-prostheses with rotation and sliding mobility and pseudo-sled-prostheses (better rotation-segment-prostheses).
(13) When the MAbs produced against CDV were tested, 37 of 39 antibodies reacted with a virus isolated from a sled dog diseased in an outbreak of distemper in Greenland prior to the epizootic among seals in the North Sea.
(14) Some were mounted in a rearward firing sled; others were placed in standard cars during collisions.
(15) David Cameron was a master stunt-artist: the husky-sledding in the Arctic circle, the bicycle-riding to Westminster.
(16) They had provisions for several more weeks on the ice, the first leg of a year-long expedition (named "180 Degrees") from geomagnetic north pole to geomagnetic south pole by dog sledding, sailing and cycling.
(17) So, even after a massive snow fall, we don’t get much time to enjoy its pleasures – digging out igloos once the storm has passed, pretending we’re Laura Ingalls Wilder and trying to make maple candy in the snow , sledding down that one big hill.
(18) Coming from the position of being a high Tory with great personal wealth and aristocratic family ties, Cameron needed to ride a husky sled across a glacier and go on about global warming to persuade people he was half-way normal.
(19) Posterior fixation of the sled may be difficult, as the sled tends to migrate anteriorly.
(20) A canine distemper outbreak in a highly susceptible sled dog population of Northern Greenland was recognized in the beginning of January 1988.