(n.) A white crystalline nitrogenous substance obtained by the action of dilute sulphuric acid on silk gelatin.
Example Sentences:
(1) Addition in the cultures of 4-deoxypyridoxine, a potent antagonist of vitamin B6 coenzymes, concurrently with the mitogen, inhibits the induction of serine hydroxymethyltransferase.
(2) Amino acid analysis indicated a significant number of serine amino acids: N-terminal sequence data demonstrated a high level of homology; and trypsin digestion followed by reversed-phase HPLC indicated the possibility of multiple phosphorylation sites.
(3) Plasminogen activator inhibitor-1 (PAI-1), the primary physiological inhibitor of tissue-type plasminogen activator (t-PA) in plasma, is a serine proteinase inhibitor (serpin) that forms a 1:1 stoichiometric complex with its target proteinase leading to the formation of a stable inactive complex.
(4) The alterations in DS frontal cortex included decreases in (n-6) and increases in (n-3) groups in choline and ethanolamine phosphoglycerides (CPG and EPG), as had previously been found in EPG and serine phosphoglyceride (SPG) of the DS fetal brain.
(5) A significant proportion of the soluble protein of the organic matrix of mollusk shells is composed of a repeating sequence of aspartic acid separated by either glycine or serine.
(6) Serine (12.0-18.2%), tyrosine (5.8-9.0%) and glycine (4.5-7.1%), along with arginine, make up the bulk of the amino acid residues in these molecules.
(7) Serine inhibited the ethanolamine incorporation by this preparation and ethanolamine inhibited the serine incorporation.
(8) The exceptions were the levels of threonine, glutamine, serine and histidine, which were three times greater in brain than in serum.
(9) Phosphorylation of serine occurs mostly (Sp H1) or entirely (Sp H2B) on the N-terminal portions of these molecules.
(10) The structural region contains serines, threonines, and cysteines at exactly the positions required to give mature nisin by a series of post-translational modifications involving dehydration of serines and threonines to dehydro forms, and cross-linking with cysteine residues.
(11) Two-dimensional phosphopeptide map analysis revealed that the major sites of tyrosine and serine phosphorylation in PLC-gamma 1 from activated Jurkat cells are the same as those in PLC-gamma 1 from cells treated with peptide growth factors such as epidermal growth factor and platelet-derived growth factor.
(12) Phosphate appears to be incorporated solely into serine residues.
(13) Other amino acids (glutamine, taurine, asparagine, alanine, serine) were only altered in single brain regions, or were not altered at all (aspartate, glycine, threonine, arginine).
(14) To gain further insight into the side chain requirement at position 177 that confers maltose recognition, further substitutions of isoleucine, leucine, phenylalanine, proline, and serine have been made via site-directed mutagenesis.
(15) Phosphoamino acid analysis showed that histidyl-tRNA synthetase is phosphorylated on serine, as has previously been shown for threonyl-tRNA synthetase of CHO cells.
(16) We present evidence that over-expression of human plasminogen, the precursor to the serine protease plasmin, can be cytotoxic to mammalian cells.
(17) Sodium Acetate, Arginine, Cysteine, Leucine, Norleucine, Methionine and Serine (more than 50%).
(18) Pancreatic and urinary kallikreins failed to form the typical serine proteinase complex with alpha2M (alpha2-macroglobulin).
(19) Serine deletion was also shown to be position-specific.
(20) The data obtained testify to the presence in the granular fraction of the endopeptidase LTH-converting activity which is sensitive to pepstatin A, an aspartyl proteinase inhibitor as well as to chelators and a serine proteinase inhibitor.
Trypsin
Definition:
(n.) A proteolytic ferment, or enzyme, present in the pancreatic juice. Unlike the pepsin of the gastric juice, it acts in a neutral or alkaline fluid, and not only converts the albuminous matter of the food into soluble peptones, but also, in part, into leucin and tyrosin.
Example Sentences:
(1) This Mr 20,000 inhibitory activity was acid and heat stable and sensitive to dithiothreitol and trypsin.
(2) Under milder trypsin digestion conditions three resistant fragments were produced from the free protein.
(3) Treatment with trypsin gave essentially one radioactive peptide, the active site peptide, of approximately 2300 molecular weight.
(4) Benzyloxycarbonylarginine p-nitrophenyl ester and other activated esters of N-a-sustituted arginine salts may be useful reagents for introduction of trypsin-labile protecting groups into peptide fragments for purpose of polypeptide semi-synthesis.
(5) Trypsin had no effect on TGase activity when added to the cells 2 h after stimulation with a serum-containing medium.
(6) Amino acid analysis indicated a significant number of serine amino acids: N-terminal sequence data demonstrated a high level of homology; and trypsin digestion followed by reversed-phase HPLC indicated the possibility of multiple phosphorylation sites.
(7) Insulin incubation of plasma membranes pretreated with protease inhibitors (leupeptin, phenylmethylsulfonylfluoride) or with exogenous trypsin, but not chymotrypsin substrates (esters of arginine and tyrosine) yields an inactive supernatant on PDH.
(8) Conflicting reports exist on the postprandial response of serum cationic trypsin like immunoreactivity (SCTLI).
(9) The activation of calcium uptake by increasing concentrations of trypsin was paralleled by the reduction of phosphorylation of phospholamban.
(10) In the alpha 2M-thrombin, alpha 2M-plasmin, and alpha 2M-trypsin complexes, approximately 50%, 60%, and 75% of the subunits are cleaved, respectively.
(11) The presence or absence of 1,8-anilinonaphthalenesulfonic acid during labeling caused no change in the peptide maps of either lectin when digested with trypsin.
(12) Binding activity was labile to heat, and to treatment with pepsin or trypsin.
(13) Such gel was analyzed for its degree of substitution, and gels with three different degrees of substitution were used in chromatographic experiments with dextranase, alpha-amylase, lactate dehydrogenase, alpha-chymotrypsin and trypsin.
(14) Affinity-purified human placental ribonuclease inhibitor (PRI) was digested by trypsin.
(15) Gastrin-34-LI in antral extracts could be converted to gastrin-17-LI by trypsin in all species.
(16) Inhibitors of DC clustering, including trypsin, paraformaldehyde, and tunicamycin, abrogated the ability of DC to support antigen presentation and lectin-mediated proliferation.
(17) Optimal myocyte cultures were obtained using serial 0.2% crude trypsin digestions of hearts from 1-2-day-old rats.
(18) Limited trypsin digestion of purified platelet GPIIIa yielded a mixture of two-chain molecules comprised of an N-terminal fragment disulphide-bonded to one of four fragments, which began at residues 299, 303, 353 or 423.
(19) Antigenic properties of crystalline pepsin, trypsin and chymotrypsin were studied in 9 rabbits immunised with these enzymes.
(20) The cytoplasmic moiety of the inverted EII could be removed with trypsin without effecting the integrity of the liposomal membrane.