(1) The cdrk channel appears to be a member of the Shab subfamily, most closely resembling drk1.
(2) The cloning of RK5 confirms the presence in mammals of all four Drosophila K+ channel families: Shaker, Shab, Shaw, and Shal.
(3) This conservation of the K+ channel subfamilies Shaker, Shal, Shab, and Shaw suggests that not only the broad outlines of membrane electrical properties but also many molecular details as well evolved in the parent species ancestral to both invertebrate and vertebrate life.
(4) The Drosophila Shaker gene on the X chromosome has three sister genes, Shal, Shab, and Shaw, which map to the second and third chromosomes.
(5) This organization precludes the generation of multiple forms of the protein by alternative RNA splicing, a mechanism known to characterize the Drosophila potassium channel genes Shaker and Shab.
(6) Homologs of Shaker, Shal, Shab, and Shaw are present in mammals; each Drosophila potassium-channel gene may be represented as a multigene subfamily in mammals.
(7) In peripheral tissues, mRNAs for cdrk and drk1 are reciprocally localized, indicating that the K+ channel properties contributed by mammalian Shab homologs may be important in a variety of excitable tissues.
(8) In Drosophila, the transient (A current) subtype of the potassium channel (Shaker and Shal) and the delayed-rectifier subtype (Shab and Shaw) are encoded by homologous genes, and there is more than one gene for each subtype of channel.
(9) Mouse and Drosophila Shab K+ channels (mShab and fShab, respectively) represent an instance of K+ channels and structurally related species that are both functionally and structurally conserved; most kinetic, voltage-sensitive, and pharmacological properties are similar for the 2 channels.
(10) We show that Drosophila Shaker, Shal, Shab, and Shaw subunits form functional homomultimers, but that a molecular barrier to heteropolymerization is present.
(11) One of these proteins (NGK2) is structurally more closely related to the Drosophila Shaw gene product than to the Shaker and Shab gene products, whereas the other (NGK1) is identical with a rat brain potassium channel protein (BK2) which is more closely related to the Drosophila Shaker gene product.
(12) The family consists of four subfamilies: ShI genes are homologues of Shaker; ShII, ShIII, and ShIV are homologues of three other Shaker-like genes in Drosophila, Shab, Shaw, and Shal, respectively.
(13) Structurally, drk1 encodes an amino-acid sequence which is more closely related to the Drosophila Shab gene than to the Shaker gene.
(14) Shaker, Shal, Shab, and Shaw K+ channels have similar structures, but appear to be independent channel systems: when co-expressed in Xenopus oocytes, all four function independently.
(15) The deduced proteins of Shab, Shaw, and Shal have high homology to the Shaker protein; the sequence identity of the integral membrane portions is greater than 50 percent.
(16) By using a Shaker complementary DNA probe and low-stringency hybridization, three additional family members have now been isolated, Shab, Shaw, and Shal.
(17) We have cloned and expressed a mouse brain K+ channel that is the homolog of the Drosophila Shab K+ channel.
Slab
Definition:
(n.) A thin piece of anything, especially of marble or other stone, having plane surfaces.
(n.) An outside piece taken from a log or timber in sawing it into boards, planks, etc.
(n.) The wryneck.
(n.) The slack part of a sail.
(a.) Thick; viscous.
(n.) That which is slimy or viscous; moist earth; mud; also, a puddle.
Example Sentences:
(1) Multiple overlapping thin 3D slab acquisition is presented as a magnitude contrast (time of flight) technique which combines advantages from multiple thin slice 2D and direct 3D volume acquisitions to obtain high-resolution cross-sectional images of vessel detail.
(2) This study investigates the photoneutron field found in medical accelerator rooms with primary barriers constructed of metal slabs plus concrete.
(3) In order to study the effects of different glass ionomers on the metabolism of Streptococcus mutans, test slabs of freshly mixed conventional glass ionomer (Fuji), silver glass ionomer (Ketac-Silver), composite (Silux), and 2-week-old Fuji were fitted into the bottom of a test tube.
(4) The native phosphoprotein has an approximate sedimentation coefficient of 14.8 S. On sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis, the protein dissociated into identical subunits of Mr = 128,000.
(5) Through small and large acts of deprivation and destruction we follow the process: the removal of hope, of dignity, of luxury, of necessity, of self; the reduction of a man to a hoarder of grey slabs of bread and the scrapings of a soup bowl (wonderfully told all this, with a novelist's gift for detail and sometimes very nearly comic surprise), to the confinement of a narrow bed – in which there is "not even any room to be afraid" – with a stranger who doesn't speak your language, to the cruel illogicality of hating a fellow victim of oppression more than you hate the oppressor himself – one torment following another, and even the bleak comfort of thinking you might have touched rock bottom denied you as, when the most immediate cause of a particular stress comes to an end, "you are grievously amazed to see that another one lies behind; and in reality a whole series of others".
(6) Stains-All treatment of slab gels showed that the cross-reactive peptide stained metachromatically blue, similarly to SR CS.
(7) The serum material extracted from the slab gel was purified from SDS and further fractionated by reverse-phase high performance liquid chromatography (HPLC).
(8) It is suggested that (1) in the chronically neuronally isolated cortical slab there is normally no spontaneous adrenergic activity, (2) a cortical, cholinergic inhibitory mechanism, previously described, is modulated by ascending adrenergic influences, (3) adrenergic cholinergic linkages might be arranged in the cortex in an alternating network, as proposed by Feldberg.
(9) These proteins were further separated on slab SDS gels and protein bands were excised after Coomassie Brilliant Blue R-250 staining and used to inject three rabbits.
(10) A system for analyzing covalent modifications of elongation factor-2 (eEF-2) by one-dimensional isoelectric focusing in slab polyacrylamide gels is described.
(11) Slabs were re-embedded in resin and 5 microns sections cut for routine undecalcified histological staining.
(12) A new prealbumin plasma esterase was demonstrated by the use of miniaturized polyacrylamide slab gel electrophoresis.
(13) Many neurons of this longitudinal slab which we have named the basal forebrain cell column, originate from an ependymal matrix closely associated with the ventral diencephalic sulcus and later become associated with the basal forebrain bundle.
(14) The water inside the channel was considered through a continuum medium using the dielectric constant of the bulk, and the membrane contribution was included using the virtual images of the pore in a dielectric slab of epsilon = 3.
(15) Protein band positions on a slab polyacrylamide gel were determined without staining, by blotting the bands onto a nitrocellulose membrane which was then stained to visualize the electrophoretic pattern.
(16) The front surface slab-off and bicentric produce base-up prism in the lower section of the lens.
(17) A small air cavity to measure ionization is embedded in the polystyrene slab at the boundary facing the backscatterer.
(18) Resembling a billhook, with Foule Crag its wickedly curved tip, this final flourish looks daunting but can be skirted to one side, up awkward slabs.
(19) Sandwood Bay in Scotland Photograph: Alamy Am Buachaille, a rocky sea stack, stood guard-like to one side, the giant grey slabs which cut into the sea were bathed in frothing waves, and the dim glow of the Cape Wrath lighthouse sent out a muted white beam beyond the cliffs to my right.
(20) The occurrence of molecular forms in protease-solubilized AChE was investigated by means of centrifugation analysis and slab gel electrophoresis.