(1) Chromatographic separation revealed that the bulk (85%) of the mitogenic activity in SSV-transformed NRK cells was not due to p28v-sis but rather two distinct endothelial cell growth factors that eluted off heparin-Sepharose between 1 and 2 M NaCl.
(2) To get an insight into the nature of variant Ph translocations and the process of their formation, we examined the localization of the c-abl and c-sis oncogenes and the breakpoint cluster region (bcr) gene by chromosomal in situ hybridization in ten variant Ph translocations of CML including five simple and five complex ones as initially interpreted.
(3) Loss of a c-sis allele (allele I in all cases) was observed in 6 out of 10 tumors from heterozygous patients.
(4) Cells transformed by v-sis produce a platelet-derived growth factor-related molecule which is able to stimulate the platelet-derived growth factor receptor in an autocrine fashion.
(5) The v-sis oncogene of simian sarcoma virus (SSV) is a retroviral version of the PDGF B chain gene and SSV-transformation is mediated by an autocrine PDGF-like growth factor.
(6) Messenger RNA levels for v-sis were induced by tension in intact but not denuded vessels.
(7) In both loci, similar unique genetic sequences were found upstream of the v-sis homologous region and these hybridized to a 4.2 kbp c-sis transcript in human lung tumor cells.
(8) First, we investigated the expression of c-sis protooncogenes within cultured human glioma cell lines and also fresh glioma specimens by using polymerase chain reaction.
(9) The v-sis gene encodes chain B of platelet-derived growth factor.
(10) However, in tissue adjacent to 5 different tumors, approximately the same level of c-sis mRNA was seen.
(11) c-sis mRNA levels diminished with increased time of infection.
(12) For example, platelet-derived growth factor (PDGF) has been found in many normal tissues including the CNS, while minor changes in one of its forms (PDGF-B) converts it to the v-sis oncogene, capable of inducing sarcomas and astrocytomas in primates.
(13) SIS SVB was performed to a variety of vessel combinations using "Y" graft, continuous, or vein extension techniques achieving early patency in all limbs, despite pedal arch disease.
(14) Radiolabeled recombinant PDGF (c-sis) dissociated from Heparin-Sepharose within a concentration range of NaCl similar to that of RF I.
(15) increased cell size, reduced growth rate, megakaryocytic antigens, and expression of the sis proto-oncogene, the structural gene for the B-chain of platelet-derived growth factor.
(16) In human malignant mesothelioma cell lines elevated expression of the platelet-derived growth factor (PDGF) beta-chain (c-sis) gene was previously reported, while normal mesothelial cells barely express this gene.
(17) The same temporal dissociation was observed when a recombinant v-sis product was used instead of porcine PDGF.
(18) Reduced function of runt results in female-specific lethality and sexual transformation of XX animals that are heterozygous for Sxl or sis loss-of-function mutations.
(19) Several specific related questions are addressed in this discussion: Is the protein encoded by the v-sis gene functionally identical to PDGF?
(20) This suggests that H-ras is less efficient in relieving the insulin requirement than is sis.
Wis
Definition:
(adv.) Certainly; really; indeed.
(v. t.) To think; to suppose; to imagine; -- used chiefly in the first person sing. present tense, I wis. See the Note under Ywis.
Example Sentences:
(1) At the Marshfield Clinic, however, a group practice in Marshfield, Wis., physicians did not know the source of payment for the vast majority of their patients (79.3 percent).
(2) Forested areas adjacent to Milwaukee, Wis., and Chicago, Ill., were investigated for rodents and ticks infected with Borrelia burgdorferi, the causative agent of Lyme disease.
(3) Because of our slightly younger average age and city location, we were supposedly one of the "new wave" WIs that had started springing up in the years before – groups that rejected crochet and did more modern activities, often with more than a tinge of irony.
(4) Eimeria tenella strain Wis-F is known to develop in chickens with a significantly shortened prepatent period and its pathogenicity is virtually completely attenuated.
(5) The inhibitory effect of L-lysine on penicillin biosynthesis by Penicillium chrysogenum has been compared in a low-producing strain (Wis. 54-1255) and a high-producing strain (ASP-78).
(6) The first-round demonstration at Marshfield, Wis, was operational for 28 months.
(7) The immunizing abilities of the attenuated line and its parent were compared by priming groups of chickens with numbers of oocysts of WisF96 or Wis, designed to produce infections of equal magnitude in terms of oocysts production (standard inocula), and then challenging with oocysts of Wis.
(8) We prospectively studied all transfers from community hospitals to the Zablocki Veterans Administration Medical Center in Milwaukee (Wis) between May 28, 1986, and January 1, 1987.
(9) This family of retroelements (termed WIS-2) occurs in the genomes of barley, wheat, rye, oats, and Aegilops species.
(10) There are around 6,600 WIs, with 520 new groups forming in the last four years alone – many of which have been in cities (until relatively recently, the WI was restricted to rural communities).
(11) The life-cycle of a precocious and attenuated line (WisF96) of Eimeria tenella, derived from the Wisconsin (Wis) strain, contained only the first of the three generations of schizogony undergone by the parent strain.
(12) Few sporozoites from the WIS strain developed into schizonts, but numerous sporozoites from the FS139 strain developed into normal first and second generation schizonts.
(13) A total of 147 preterm pregnant women at Orlando Regional Medical Center were screened for group B streptococci by using Lim Group B Strep Broth (GIBCO Laboratories, Madison, Wis.) and the Phadebact Strep B Test (Pharmacia Diagnostics, Piscataway, N.J.).
(14) Subacute hematomas had peripheral hyperintensity on T1-WIs and then on T2-WIs.
(15) The WARF Institute, Inc. (Madison, Wis) has been preparing most of the crude plant extracts for antitumor screening for the past 14 years.
(16) The Wis-F-96 strain did not adequately immunize chickens in these experiments.
(17) Despite antibiotic therapy, four developed WIs caused by these organisms.
(18) This concept was examined in cultured, aortic VSMCs (passages 6-10) from SHR, Wistar-Kyoto (WKY), and American Wistar (Wis) rats.
(19) Bond recalls Baroness Kennedy QC speaking at the Women of the World festival in 2012 and remembering how instrumental WIs had been in getting recognition of rape within marriage on to the political agenda.
(20) Clones of P. chrysogenum Wis 54-1255 transformed with the ips gene showed a five-fold higher isopenicillin N synthase activity than the untransformed cultures.