(1) Microionophoretically applied excitatory amino acids induced firing of extracellularly recorded single units in a tissue slice preparation of the mouse cochlear nucleus, and the similarly applied antagonist 2-amino-5-phosphonovalerate (2APV) was demonstrated to be a selective N-methyl-D-aspartate (NMDA) receptor antagonist.
(2) Multiple overlapping thin 3D slab acquisition is presented as a magnitude contrast (time of flight) technique which combines advantages from multiple thin slice 2D and direct 3D volume acquisitions to obtain high-resolution cross-sectional images of vessel detail.
(3) This difference was abolished by exposure of the slices to propranolol, a beta-adrenergic receptor antagonist.
(4) All three compounds were also very similar in their effects on [3H]5HT release from superfused rat striatal slices.
(5) Intoxicating concentrations of ethanol also inhibit excitatory synaptic transmission mediated by N-methyl-D-aspartate receptors in hippocampal slices from adult rodents.
(6) The present in vitro studies show that it is found as beta-endorphin in bovine pituitary slices incubated with radioactive amino acid precursor [35S]methionine.
(7) This provides a direct display, in the viewing plane, of the slice profile.
(8) In the longitudinal direction, however, spatial resolution of under slice thickness could not be obtained.
(9) Tubules and cells were released from slices of kidney cortex by collagenase.
(10) To determine the severity of regurgitation by dynamic MRI, several parameters were analyzed, including the number of slices with visible signal loss, the time course of the signal loss, and its maximal area and maximal volume.
(11) This study evaluated the in vitro renin release, tissue cyclic AMP content (TcAMPc), and tissue renin content (TRC) changes with time, in response to administration of dopamine (DOP) and of the dopamine-receptor blocking agent pimozide (PIM) to renal cortical slices from sodium deficient (SD) rats.
(12) The Press Association tots up a total of £26bn in asset sales last year – including the state’s Eurostar stake, 30% of the Royal Mail and a slice of Lloyds.
(13) The effect of p-nitrophenylphosphate (p-NPP) on the release of acetylcholine evoked by drugs and ionic environments known to inhibit Na+, K+-ATPase was studied in isolated cortical slices of rat brain and longitudinal muscle strip of guinea-pig ileum.
(14) Prostate slices were perfused with a medium containing [(3)H]testosterone and [(14)C]androstenedione, or 5alpha-dihydro-[(3)H]testosterone and [(14)C]testosterone.
(15) The adenylate cyclase activator forskolin as well as 8-bromo-cyclic AMP enhanced the electrically evoked release of 3H-noradrenaline and 3H-5-hydroxytryptamine from superfused rat neocortical slices and that of 3H-dopamine from neostriatal slices with comparable EC50's of about 0.5 and 50 microM, respectively, without affecting spontaneous tritium efflux.
(16) Aspartate levels and release from rat striatal slices following the inhibition of glutamine synthetase (GS) by methionine sulfoximine (MSO) were studied.
(17) The effects of stimulus-evoked potassium release on the excitability of presynaptic axons were studied in the rat hippocampal slice preparation.
(18) The A1-selective agonist R-(-)N6-(2-phenylisopropyl)adenosine (10 microM) decreased 100 microM NMDA-evoked [3H]norepinephrine release by 27%; this was reversed by the P1 antagonist 8-phenyltheophylline (8-PT, 10 microM), indicating that NMDA-evoked norepinephrine release from cortical slices is susceptible to purinergic modulation.
(19) In vitro addition of denbufylline (10(-8)-10(-4) M) produced no significant change in [3H]choline uptake in striatal slices, while denbufylline (10(-4) M) increased high (20 mM) potassium-evoked endogenous ACh release from striatal slices.
(20) Under the electron microscope, slices appeared vacuolated near the cut surfaces, but well preserved internally (greater than 40 micron from the edge).
Spliced
Definition:
(imp. & p. p.) of Splice
Example Sentences:
(1) It is argued that this process drove the evolution of present 5' and 3' splice sites from a subset of proto-splice sites and also drove the evolution of a more efficient splicing machinery.
(3) One splicing mutation results in a 3 amino acid in-frame insertion.
(4) Each of the two spliced molecules contains a 5'-terminal leader sequence transcribed from a DNA segment with an estimated length of 60 to 110 nuvleotides.
(5) Previously, we identified a candidate gene, Tcp-10b, whose t allele generates alternatively spliced transcripts.
(6) Analysis of this mutant illustrates that indirect flight muscles and jump muscles utilize different mechanisms for alternative RNA splicing.
(7) A comparison between the primary structures of the rat and human genes reveals that the heterogeneity of the C-terminal peptides can be explained in large part by a frameshift mutation and the utilization of an alternative splice donor site in the third exon of the rat gene.
(8) A new alternative splice site was incidently found 81 nucleotide downstream of motif II in both normal and truncated 4.1 mRNA.
(9) The IGA-65 present in splicing extracts was distinguishable from known U-snRNP and hnRNP proteins on protein gels.
(10) The protein variation potentially includes N-terminal differences coded for by transcript-specific 5' exons and internal differences arising from the optional inclusion of a 39 base-pair exon and from the alternative use of two 3' splice sites separated by six base-pairs.
(11) Certain RNA molecules can mediate their own cleavage or splicing or act as enzymes to promote reactions on substrate RNA molecules.
(12) In order to identify putative protein splicing factors associated with the 200S InRNP particles, a panel of monoclonal antibodies directed against these particles were screened for their ability to inhibit splicing of pre-mRNA in vitro.
(13) Similar results were obtained when hybrid-selected RNA, which had been labeled for 3 h with [3H]uridine, was analyzed on formaldehyde-agarose gels, suggesting that the observed differences were due to splicing rather than RNA stability.
(14) At the restrictive temperature the ratio of unspliced U6 precursor to mature RNA is elevated in the mutants compared to the wild type grown under identical conditions, indicating a defect in U6 pre-RNA splicing.
(15) These two splicing intermediates cosedimented under a variety of conditions, indicating that they are contained in the same RNP complex.
(16) Recombinant retroviruses containing intact or spliced CRPV sequences can now be used to introduce the viral genes efficiently into a variety of cell lines.
(17) The 0.85 kb fragment was cloned and sequenced and the result indicated that the introns from the xylanase gene were accurately spliced by the plant cells.
(18) The RNA-catalysed self-splicing reaction of group II intron RNA is assumed to proceed by two consecutive transesterification steps, accompanied by lariat formation.
(19) U1 snRNA is an essential splicing factor known to base pair with 5' splice sites of premessenger RNAs.
(20) We have used such constructs, assayed by transient expression in chicken embryo fibroblasts, to define the minimal sequences necessary to inhibit splicing.