(1) The insulin receptor made in the presence of the inhibitor can be affinity labeled at the cell surface using 125I-insulin and disuccinimidyl suberate cross-linking; there is also no significant difference in its affinity for insulin.
(2) 18F labeling was performed using N-succinimidyl-8-(4-[18F]fluorobenzylamino) suberate.
(3) Affinity cross-linking experiments with the homobifunctional cross-linking reagents disuccinimidyl suberate, disuccinimidyl tartrate, and dithiobis(succinimidyl propionate) resulted in the identification of a receptor protein with a Mr of 130,000 on five out of the seven cell types examined.
(4) The affinity cross-linking of the delta-opioid receptor in neuroblastoma x glioma NG108-15 cells was undertaken using (3-[125I]iodotyrosyl27)human-beta-endorphin ([125I]beta-endorphin) and disuccinimidyl suberate (DSS) or bis(sulfosuccinimidyl) suberate (BS3) in order to estimate molecular size.
(5) The interaction of S-100 protein with the above proteins was investigated by fluorescence spectroscopy using acrylodan-S-100 protein and acrylodan-annexin II and by cross-linking experiments using the bifunctional cross-linker disuccinimidyl suberate (DSS).
(6) Following cross-linking with disuccinimidyl suberate and analysis by SDS-PAGE and autoradiography, both the high- and low-affinity TSH binding components exhibited two similar 125I-TSH-labeled bands, with Mr values of 80,000 and 68,000.
(7) From the fresh roots of Trichosanthes bracteata Voigt., the following substances were identified: methyl palmitate, palmitic acid, suberic acid, alpha-spinasterol, stigmast-7-en-3 beta-ol, alpha-spinasterol 3-O-beta-D-glucopyranoside, stigmast-7-en-3 beta-ol 3-O-beta-D-glucopyranoside, glyceryl 1-palmitate, glyceryl 1-stearate, bryonolic acid, cucurbitacin B, isocucurbitacin B, 3-epi-isocucurbitacin B, 23,24-dihydrocucurbitacin B, 23,24-dihydroisocucurbitacin B, 23,24-dihydro-3-epi-isocucurbitacin B, cucurbitacin D, isocucurbitacin D and D-glucose.
(8) The 125I-insulin-protein complexes were covalently cross-linked with disuccinimidyl suberate and chromatographed on a Sepharose CL-6B column.
(9) Radioiodinated TSH was covalently cross-linked to monolayers of FRTL-5 rat thyroid cells using the homobifunctional cross-linking agent disuccinimidyl suberate.
(10) Disuccinimidyl suberate was unable to cross-link [125I]-salmon calcitonin either on T47D or MCF cells.
(11) Kinetic studies with purified enzyme using the mono-CoA esters of sebacic (DC10-CoA), suberic (DC8-CoA), and adipic (DC6-CoA) acids along with DC12-CoA revealed substrate inhibition.
(12) The molecular characteristics of the somatostatin (SRIF) receptor were investigated by covalently cross-linking [125I-Tyr11]SRIF to rat anterior pituitary membranes using three heterobifunctional cross-linking agents, N-5-azido-2-nitrobenzoyloxysuccinimide, N-hydroxysuccinimidyl-4-azidobenzoate, and N-succinimidyl-6-(4'-azido-2'-nitrophenylamino) hexanoate, and the homobifunctional agent disuccinimidyl suberate.
(13) When purified membranes were cross-linked with 125I-rANP, using disuccinimidyl suberate and separated by SDS-PAGE, approximately 75- and approximately 140-kDa proteins were specifically labeled in a ratio of approximately 3:1, respectively.
(14) We found disulfide-linked dimers as well as noncovalently associated dimers that were detected by cross-linking with bis(sulfosuccinimidyl) suberate (BS3).
(15) Haemopexin receptors from mouse hepatoma (Hepa) cells were affinity-labelled by cross-linking to haem-125I-haemopexin complexes using two homo-[disuccinimidyl suberate (DSS) and 3,3'-dithiobis(succinimidyl propionate) (DTSSP)] and one hetero-[sulphosuccinimidyl 4-(p-maleimidophenyl)butyrate (sulpho-SMPB)] bifunctional cross-linking agents.
(16) The beta-oxidation of [1,2,3,4-13C4]hexadecanedioic acid yielded labeled adipic, suberic, and sebacic acids.
(17) [125I]VIP was covalently cross-linked to tracheal membrane proteins using disuccinimidyl suberate.
(18) The transport inhibitor DNDS (4,4'-dinitrostilbene-2,2'-disulfonate) changes the bis(sulfosuccinimidyl)suberate (BS3) crosslinking pattern of band 3 protein from a mixture of dimer-crosslinkable (DC) and tetramer-crosslinkable (TC) states to the TC-state as the exclusive crosslinked product for reactions occurring in membranes of intact human erythrocytes.
(19) An epidermal growth factor (EGF) receptor monoclonal antibody (mAb), mAb LA22, was used to analyze the covalent coupling of human EGF receptors to mouse EGF by the amine-reactive cross-linking agent disuccinimidyl suberate.
(20) The first method was to use a specific photoaffinity derivative of salmon calcitonin and the second employed the chemical cross-linker, disuccinimidyl suberate.
Tuberous
Definition:
(a.) Covered with knobby or wartlike prominences; knobbed.
(a.) Consisting of, or bearing, tubers; resembling a tuber.
Example Sentences:
(1) In addition, lightly immunostained cells were distinguished in the caudal portion of the hypothalamic arcuate nucleus, area of tuber cinereum, retrochiasmatic area, and rostral portion of the paraventricular thalamic nucleus after colchicine treatment.
(2) This paper describes a family in which the first child, a girl born in 1988, has tuberous sclerosis (TS).
(3) Tuning curves of afferent electroreceptive fibers in the anterior lateral line nerve of the weakly electric fish, Sternopygus macrurus, indicate that the tuberous electroreceptors of each individual are well-tuned to its own electric organ discharge (EOD) frequency.
(4) 100 degrees C. Thus residues did not migrate into the flesh of the tubers.
(5) Aromatase immunoreactive cells (ARO-ir) were found in the medial preoptic nucleus, in the septal region, and in a large cell cluster extending from the dorso-lateral aspect of the ventromedial nucleus of the hypothalamus to the tuber at the level of the nucleus inferioris hypothalami.
(6) Injections of horseradish peroxidase into the telencephalon retrogradely labeled neurons ipsilaterally in various thalamic, preglomerular, and tuberal nuclei, the nucleus of the locus coeruleus (also contralaterally), the superior raphe, and portions of the nucleus lateralis valvulae.
(7) Isolated nuclei from green leaf tissue of tomato plants infected with potato spindle tuber viroid (PSTVd) were bound to microscope slides, fixed with formaldehyde and hybridized with biotinylated transcripts of cloned PSTVd cDNA.
(8) A simple and efficient method is presented for the extraction, cleanup, and liquid chromatographic (LC) determination of oxamyl residues in potato tubers.
(9) The study of the selective renal arteriogram enables preoperative diagnosis of angiomyolipoma even when not associated with tuberous sclerosis, and a partial nephrectomy.
(10) The association of diffuse lipomatosis with tuberous sclerosis is reported.
(11) Developing pyramidal cells in the remaining three tuberous organ-receptive lateral ELL segments are unreactive.
(12) CD rats were much less responsive to mPOA stimulation (spaced electrodes) than O-M rats, but the responses of both strains to tuberal stimulation were essentially alike.
(13) It did not allow distinction between true pachygyria, focal cortical dysplasia, or the forme fruste of tuberous sclerosis.
(14) cDNA clones of potato virus X (PVXcp strain), potato virus Y (PVYo strain), potato leaf roll virus (PLRV) and potato spindle tuber viroid (PSTV) were used separately or combined for the detection of the corresponding RNAs in extracts of infected plants.
(15) The export of pectate lyase, polygalacturonase, and cellulase and the maceration of potato tuber tissue occurred with Out+, but not Out-, strains of E. carotovora subsp.
(16) We conclude that neurons in the lateral tuberal nucleus show an early stage of AD-related cytoskeletal pathology (Alz-50 positivity), but without plaques or neuronal death.
(17) The possibility of incorporating Icacinia manni among the edible starchy plant tubers is discussed.
(18) Western blots of extracts from P(i)-deficient cells were probed with rabbit anti-(potato tuber PFP) immune serum and revealed equal intensity staining immunoreactive polypeptides of M(r) 66,000 (alpha-subunit) and 60,000 (beta-subunit) that co-migrated with the alpha- and beta-subunits of homogeneous potato tuber PFP.
(19) In six patients the clinical symptoms were atypical; in those instances computed tomography makes diagnosis of tuberous sclerosis possible.
(20) Angiomyolipomas produce pathognomonic appearances on modern imaging methods and a tissue diagnosis is no longer required particularly when multiple tubers can be confidently diagnosed and if a CT brain scan shows periventricular calcifications.