(1) The insulin receptor made in the presence of the inhibitor can be affinity labeled at the cell surface using 125I-insulin and disuccinimidyl suberate cross-linking; there is also no significant difference in its affinity for insulin.
(2) 18F labeling was performed using N-succinimidyl-8-(4-[18F]fluorobenzylamino) suberate.
(3) Affinity cross-linking experiments with the homobifunctional cross-linking reagents disuccinimidyl suberate, disuccinimidyl tartrate, and dithiobis(succinimidyl propionate) resulted in the identification of a receptor protein with a Mr of 130,000 on five out of the seven cell types examined.
(4) The affinity cross-linking of the delta-opioid receptor in neuroblastoma x glioma NG108-15 cells was undertaken using (3-[125I]iodotyrosyl27)human-beta-endorphin ([125I]beta-endorphin) and disuccinimidyl suberate (DSS) or bis(sulfosuccinimidyl) suberate (BS3) in order to estimate molecular size.
(5) The interaction of S-100 protein with the above proteins was investigated by fluorescence spectroscopy using acrylodan-S-100 protein and acrylodan-annexin II and by cross-linking experiments using the bifunctional cross-linker disuccinimidyl suberate (DSS).
(6) Following cross-linking with disuccinimidyl suberate and analysis by SDS-PAGE and autoradiography, both the high- and low-affinity TSH binding components exhibited two similar 125I-TSH-labeled bands, with Mr values of 80,000 and 68,000.
(7) From the fresh roots of Trichosanthes bracteata Voigt., the following substances were identified: methyl palmitate, palmitic acid, suberic acid, alpha-spinasterol, stigmast-7-en-3 beta-ol, alpha-spinasterol 3-O-beta-D-glucopyranoside, stigmast-7-en-3 beta-ol 3-O-beta-D-glucopyranoside, glyceryl 1-palmitate, glyceryl 1-stearate, bryonolic acid, cucurbitacin B, isocucurbitacin B, 3-epi-isocucurbitacin B, 23,24-dihydrocucurbitacin B, 23,24-dihydroisocucurbitacin B, 23,24-dihydro-3-epi-isocucurbitacin B, cucurbitacin D, isocucurbitacin D and D-glucose.
(8) The 125I-insulin-protein complexes were covalently cross-linked with disuccinimidyl suberate and chromatographed on a Sepharose CL-6B column.
(9) Radioiodinated TSH was covalently cross-linked to monolayers of FRTL-5 rat thyroid cells using the homobifunctional cross-linking agent disuccinimidyl suberate.
(10) Disuccinimidyl suberate was unable to cross-link [125I]-salmon calcitonin either on T47D or MCF cells.
(11) Kinetic studies with purified enzyme using the mono-CoA esters of sebacic (DC10-CoA), suberic (DC8-CoA), and adipic (DC6-CoA) acids along with DC12-CoA revealed substrate inhibition.
(12) The molecular characteristics of the somatostatin (SRIF) receptor were investigated by covalently cross-linking [125I-Tyr11]SRIF to rat anterior pituitary membranes using three heterobifunctional cross-linking agents, N-5-azido-2-nitrobenzoyloxysuccinimide, N-hydroxysuccinimidyl-4-azidobenzoate, and N-succinimidyl-6-(4'-azido-2'-nitrophenylamino) hexanoate, and the homobifunctional agent disuccinimidyl suberate.
(13) When purified membranes were cross-linked with 125I-rANP, using disuccinimidyl suberate and separated by SDS-PAGE, approximately 75- and approximately 140-kDa proteins were specifically labeled in a ratio of approximately 3:1, respectively.
(14) We found disulfide-linked dimers as well as noncovalently associated dimers that were detected by cross-linking with bis(sulfosuccinimidyl) suberate (BS3).
(15) Haemopexin receptors from mouse hepatoma (Hepa) cells were affinity-labelled by cross-linking to haem-125I-haemopexin complexes using two homo-[disuccinimidyl suberate (DSS) and 3,3'-dithiobis(succinimidyl propionate) (DTSSP)] and one hetero-[sulphosuccinimidyl 4-(p-maleimidophenyl)butyrate (sulpho-SMPB)] bifunctional cross-linking agents.
(16) The beta-oxidation of [1,2,3,4-13C4]hexadecanedioic acid yielded labeled adipic, suberic, and sebacic acids.
(17) [125I]VIP was covalently cross-linked to tracheal membrane proteins using disuccinimidyl suberate.
(18) The transport inhibitor DNDS (4,4'-dinitrostilbene-2,2'-disulfonate) changes the bis(sulfosuccinimidyl)suberate (BS3) crosslinking pattern of band 3 protein from a mixture of dimer-crosslinkable (DC) and tetramer-crosslinkable (TC) states to the TC-state as the exclusive crosslinked product for reactions occurring in membranes of intact human erythrocytes.
(19) An epidermal growth factor (EGF) receptor monoclonal antibody (mAb), mAb LA22, was used to analyze the covalent coupling of human EGF receptors to mouse EGF by the amine-reactive cross-linking agent disuccinimidyl suberate.
(20) The first method was to use a specific photoaffinity derivative of salmon calcitonin and the second employed the chemical cross-linker, disuccinimidyl suberate.
Uberous
Definition:
(a.) Fruitful; copious; abundant; plentiful.
Example Sentences:
(1) In January, Paris taxi drivers attacked an Uber car transporting two passengers from Charles de Gaulle airport.
(2) When I eventually get hold of a human at Uber, I am told the only insurance cover is up to $1m to cover “bodily injury or property damage to third parties where the claim arises out of UberEats and UberRush operations”.
(3) Prosecutors in San Francisco and Los Angeles alleged that it was false for Uber to say it was the leader in screening drivers when its background checks were inferior to the process taxi drivers undergo, since Uber does not include fingerprint checks.
(4) What we’d like to take off the table is the assumption that the sharing and on-demand economy is not profitable,” says Ferguson, “and if we were to impose any additional costs they’d go out of business.” Some experts say it’s only a matter of time before companies like Uber have to change the way they employ people.
(5) The company recently announced its ambition to reach a valuation of $50bn, but it is unclear how much Uber is worth if it has to start picking up expenses it has up to now pushed on to the shoulders of its drivers.
(6) Didi Chuxing also claims it accounts for 87% of China’s ride-hailing market, in which US-based Uber is trying to break through.
(7) US senator Al Franken also said this week that Uber had failed to respond adequately to questions he submitted last month about its privacy policies.
(8) It is bad enough that the minimum wage required by law is hardly generous, yet there we were again last week confronted with reports of delivery company Hermes exploiting workers , HM Revenue & Customs widening its investigation into the notorious wages shirker Sports Direct and a challenge to Uber’s employment practices.
(9) That’s why we need to ensure that the language used to describe safety at Uber is clear and precise.” In a letter to the San Diego airport authority sent last year, the company pointed out that in some ways, it exceeds standard safety requirements.
(10) When several of its semi-autonomous cars were caught running red lights, the state ordered their removal from the road – an order Uber openly defied , blaming the traffic light violations on “human error” and suspending the people monitoring the cars.
(11) I wrote to Uber, which stated that it adds a 2.6 surcharge on days when there is likely to be a lot of demand – it was Ladies’ Day.
(12) The head of the New South Wales taxi council has lashed out at Labor leader Luke Foley’s support for Uber, likening the system to “WorkChoices on steroids”.
(13) Uber drivers are employees not contractors, California rules Read more Like many Ethiopian immigrants in San Diego , Sahilu gravitated towards driving a cab because he didn’t speak much English and couldn’t get recognition for his educational qualifications – in his case, a chemistry degree.
(14) [Uber said it didn’t need permits “since the cars have people in them monitoring movements”.
(15) He has been elevated to the role of chief adviser to the company and its chief executive Travis Kalanick, and will also sit on the board of Uber .
(16) TfL , which has come under pressure from black-cab drivers, who feel Uber has been getting too easy a time from the regulators, is running the consultation until 23 December.
(17) Police last week raided Uber offices in the southern city of Guangzhou, seizing thousands of iPhones and other equipment used to run the business.
(18) Every violation by Uber will be evaluated and we will go for legal recourse,” said Madhur Verma, the Delhi police deputy commissioner.
(19) All of the five drivers I spoke to had left minicab firms to join Uber, including one, Riz, 36, who had left Addison Lee, and they all felt that they were now being treated better.
(20) But since my wife now works at Uber , it’s probably just as well I can’t use it.