(v. t.) To write over again, or in the same words; to copy; as, to transcribe Livy or Tacitus; to transcribe a letter.
Example Sentences:
(1) RNA transcribed in vitro from the early region of bacteriophage T3 or T7 was translated by cytoplasmic ribosomes which synthesized protein in cell-free systems prepared from mammalian cells and wheat germ.
(2) The gene, which is located at chromosome XIII, is transcribed as a mRNA of about 2.7 kilobases, and the amount of message has been found to increase 3- to 4-fold during the culture.
(3) RNA preparations were reverse-transcribed, amplified by a polymerase chain reaction (PCR), and separated by electrophoresis.
(4) Early after infection, the E3 promoter is used to make mainly mRNAs a and h. Late after infection, the E3 promoter appears to be shut off and the major late promoter is used to make mainly mRNAs d and e. The late L4 mRNA 3' end site is not used early even though early E3 pre-mRNAs transcribe through the L4 RNA 3' end site.
(5) Thus, hyp does not appear to affect metastable variation but does affect the level of transcription of the pilA gene in the ON (transcribed) mode.
(6) MDR1-containing amplicons may include a number of additional transcribed genes that do not appear to contribute to multidrug resistance.
(7) In vivo, ribosomal RNA of Saccharomyces cerevisiae is transcribed from the light strand of gamma DNA.
(8) Each of the two spliced molecules contains a 5'-terminal leader sequence transcribed from a DNA segment with an estimated length of 60 to 110 nuvleotides.
(9) Sequences representing the entire TIR are transcribed into poly(A)+ mRNA at both early and late times in the infection.
(10) VZV TK templates were linearized at internal restriction sites and RNAs transcribed from these templates directed the synthesis of polypeptides with sizes consistent with the colinearity of the VZV TK gene.
(11) Oligodeoxynucleotides related to the non-transcribed DNA strands can effectively inhibit the RNA synthesis catalyzed by E. coli RNA polymerase.
(12) To identify the molecular defect in a patient with the intermediate form of the disease, arylsulfatase B mRNA from his fibroblasts was reverse-transcribed, amplified by the polymerase chain reaction, and subcloned.
(13) Similar sequence periodicities are found in the internal control regions of other genes transcribed by RNA polymerase III, and also in the SV40 promoter and a monkey gene region to which the transcription factor Sp1 binds.
(14) Nevertheless, XA is transcribed into a stable 2.6-kb polyadenylated RNA that is expressed uniquely in the adrenal gland.
(15) To determine which enzymes are responsible for the processing cleavages of ribosomal RNA transcripts in Escherichia coli, we constructed a mutant strain lacking RNAase III and containing a thermolabile RNAase P. At the nonpermissive temperature, this strain accumulates a novel "19S" RNA species which contains 17S precursor rRNA sequences covalently linked to tRNA sequences transcribed from the ribosomal RNA spacer region between the 16S and the 23S rRNA cistrons.
(16) RNA transcribed by RNA polymerase II on denatured DNA was only large RNA around 28S.
(17) Expression of the N-methyl-D-aspartate receptor gene during long-term administration of competitive and non-competitive NMDA antagonists was studied in rat brain using antisense cRNA transcribed from reverse transcriptase-polymerase chain reaction (RT-PCR)-generated rat NMDA receptor cDNA.
(18) In addition, the transcriptional behaviour of this tRNALeuCUG gene in various in vitro systems is described and it seems that, although the gene is transcribed in all test systems, the very A + T-rich 5'-flanking sequence of this particular gene may be somewhat inhibitory to transcription in vitro.
(19) A combination of techniques for reverse transcribing mRNA into cDNA and the incorporation of 32P-gamma ATP into the polymerase chain reaction (PCR)-generated fragments allowed us to detect beta 2-AR mRNA in surgically excised thyroid specimens.
(20) A new repetitive DNA region was identified in the non-transcribed spacer of human rDNA, namely a long (4.6 kb) sequence motif (Xbal element) was present in two copies.
Transcript
Definition:
(n.) That which has been transcribed; a writing or composition consisting of the same words as the original; a written copy.
(n.) A copy of any kind; an imitation.
(n.) A written version of what was said orally; as, a transcript of a trial.
Example Sentences:
(1) These lysates are comparable to those of Escherichia coli in transcriptional and translational fidelity and efficiency in response to a given template DNA.
(2) This induction is sensitive to actinomycin D but not to protein synthesis inhibitor puromycin, indicating an effect of estradiol at the transcriptional level, possibly mediated by the estrogen receptor.
(3) The promoters of the adenovirus 2 major late gene, the mouse beta-globin gene, the mouse immunoglobulin VH gene and the LTR of the human T-lymphotropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected human T cell line producing viral proteins).
(4) Quantitative determinations indicate that the amount of PBG-D mRNA is modulated both by the erythroid nature of the tissue and by cell proliferation, probably at the transcriptional level.
(5) Here we show that this induction of AP-2 mRNA is at the level of transcription and is transient, reaching a peak 48-72 hr after the addition of RA and declining thereafter, even in the continuous presence of RA.
(6) At the fepB operator, a 31 base-pair Fur-protected region was identified, corresponding to positions -19 to +12 with respect to the transcriptional start site.
(7) A strong block to the elongation of nascent RNA transcripts by RNA polymerase II occurs in the 5' part of the mammalian c-fos proto-oncogene.
(8) In the absence of an authentic target for the MASH proteins, we examined their DNA binding and transcriptional regulatory activity by using a binding site (the E box) from the muscle creatine kinase (MCK) gene, a target of MyoD.
(9) Thus, human bronchial epithelial cells can express the IL-8 gene, with expression in response to the inflammatory mediator TNF regulated mainly at the transcriptional level, and with elements within the 5'-flanking region of the gene that are directly or indirectly modulated by the TNF signal.
(10) Nevertheless, this LTR does not govern efficient transcription of adjacent genes in a transient expression assay.
(11) Other DNase I hypersensitive sites located adjacent to the S14 cap site at -65 to -265 base pairs (Hss-1) or upstream at -1.3 kb (Hss-2), -2.1 kb (Hss-3'), -5.3 kb (Hss-4), and -6.2 kb (Hss-5) remained unaffected by changes in S14 gene transcription.
(12) In vitro transcription products were analyzed for their 5' end sequences and their oligonucleotide compositions.
(13) We have investigated interactions between the erythroid transcription factor GATA-1 and factors binding two cis-acting elements commonly linked to GATA sites in erythroid control elements.
(14) Thyroid hormones suppress transcription of the gene for the beta-subunit of thyrotropin (TSH beta).
(15) An sdh-specific transcript of about 3,450 nucleotides was detected in vegetative bacteria.
(16) An attempt was made to elucidate possible participation of low molecular weight nuclear RNA's (LMWN RNA's) in the transcription process.
(17) The relative contributions of transcriptional and posttranscriptional regulation of gene expression to the increase in constitutively expressed cellular proteins were examined in mouse kidneys undergoing compensatory growth following unilateral nephrectomy (UNI-NX).
(18) Reverse transcription of retina mRNA followed by DNA amplification using D4-specific nucleotides demonstrates the presence of D4 mRNA in retina.
(19) These levels are sufficient to maintain normal in vivo rates of mRNA and rRNA synthesis, but the average density of packing of polymerases on DNA is considerably less than the maximum density predicted by Miller and Bakken (1972), suggesting that initiation of polymerases of DNA is a limiting factor in the control of transcription.
(20) In addition, a 2.6-kilobase WHV RNA transcript was found in the majority of the NL tissues.