(1) Biliary and urinary excretion of five tritium-labelled cardiac glycosides, i.e.
(2) Tritium-labeled ribonucleic acid precursors, including cytidine, uridine, and orotic acid, were injected into rats with dated pregnancies (14 to 21 days) and virgin rats.
(3) Ovarian aromatase activity, assessed by incubating the homogenised ovaries of treated rats with tritium-labelled androstenedione (0.2 microM) or testosterone (1 microM), indicated that residual enzyme activity was reduced compared with controls.
(4) The adenylate cyclase activator forskolin as well as 8-bromo-cyclic AMP enhanced the electrically evoked release of 3H-noradrenaline and 3H-5-hydroxytryptamine from superfused rat neocortical slices and that of 3H-dopamine from neostriatal slices with comparable EC50's of about 0.5 and 50 microM, respectively, without affecting spontaneous tritium efflux.
(5) It was also found that tritium release also occurred, although to a lesser extent, when tissues were fixed in glutaraldehyde or osmium tetroxide using collidine as a buffer, and this release was not significant when collidine was applied to previously fixed tissues.
(6) The standardised mortality ratios were 889 (six deaths) in employees monitored for contamination by tritium, 254 (nine deaths) in those monitored for contamination by other radionuclides, and 385 (nine deaths) in those with dosimeter readings totalling more than 50 mSv (5 rem); but the same nine subjects tended to account for each of these significantly raised ratios.
(7) 3T3 cells were also used for the incorporation of tritium-thymidine.
(8) Isolated hepatocytes carry out the N-demethylation of dansylamide at near linear rates for up to 8 h. This reaction was measured by following the release of tritium into water on hydroxylation of 3H-labeled methyl groups.
(9) Similar low deuterium and tritium isotope effects were measured with a purified rat liver cytochrome P-450 preparation and horseradish peroxidase.
(10) The effects of alpha-adrenoceptor agonists and antagonists on the electrically (slices) or potassium-evoked (synaptosomes) tritium overflow were studied.
(11) During the monodirectional conversion of D-[2-3H]mannose 6-phosphate to D-fructose 6-phosphate and D-fructose 1,6-bisphosphate, the reaction velocity is one order of magnitude lower than with D-[U-14C]mannose 6-phosphate and little tritium (less than 6%) is transferred intramolecularly.
(12) The 72-hour cumulative urinary excretion of total tritium was 28.2% of the administered dose and only 38.3% of this resided in unchanged HHT.
(13) However, higher doses of diltiazem (60 microM) reduced the tritium overflow elicited by RNS or Vt but enhanced that caused by KCl.
(14) The substrate was labelled by the catalytic addition of tritium to cerebroside sulphate.
(15) In contrast, in synaptosomes superfused with Ca(2+)-free Krebs-Henseleit solution containing 15 mM K+ throughout, ethanol did not affect the tritium overflow evoked by 2 min introduction of 75 microM Ca2+ into the superfusion fluid.
(16) We have assessed a multiwire camera for the high speed, quantitative autoradiography of tritium-labelled substances in two-dimensional systems.
(17) Dipyridamole (5 x 10(-5) M) and erythro-9-2-hydroxy-3 nonyl adenosine (EHNA) (10(-4) M) also reduced tritium release.
(18) Tritium thymidine incorporation rates inform about DNA-synthesis.
(19) GR43175 (1 x 10(-8) to 1 x 10(-5) M) inhibited the electrically evoked release of tritium from renal noradrenergic nerves but did not affect the basal (non-stimulated) release of tritium or perfusion pressure.
(20) The activity of aromatase in microsomal preparations was assayed by determining the rate of incorporation of tritium from 1-[3H]androstenedione into [3H]water.
Triton
Definition:
(n.) A fabled sea demigod, the son of Neptune and Amphitrite, and the trumpeter of Neptune. He is represented by poets and painters as having the upper part of his body like that of a man, and the lower part like that of a fish. He often has a trumpet made of a shell.
(n.) Any one of many species of marine gastropods belonging to Triton and allied genera, having a stout spiral shell, often handsomely colored and ornamented with prominent varices. Some of the species are among the largest of all gastropods. Called also trumpet shell, and sea trumpet.
(n.) Any one of numerous species of aquatic salamanders. The common European species are Hemisalamandra cristata, Molge palmata, and M. alpestris, a red-bellied species common in Switzerland. The most common species of the United States is Diemyctylus viridescens. See Illust. under Salamander.
Example Sentences:
(1) The enzyme was solubilized by Triton X-100 and purified approximately 480-fold by gel filtration and affinity chromatography on alanine methyl ketone-AH-Sepharose 4B.
(2) In the Triton-induced hyperlipidemia, 50 mg BR-931 per kg was equieffective as 200 mg of clofibrate (CPIB) per kg.
(3) This enzyme was purified to homogeneity and exhibited an apparent molecular weight of 36,000 on sodium dodecyl sulfate gels and 180,000 on a TSK G-3000SW column in the presence of Triton X-100.
(4) Isoprenylated proteins were enriched in the detergent phase upon partition with the non-ionic detergent Triton X-114.
(5) Enzyme activities were measured on nitrocellulose blots by using pure enzyme preparations as well as Triton X-100-solubilized membranes.
(6) Receptors solubilized with Triton X-100 eluted in gel filtration as a major peak of Mr = 330,000 and retained high affinity binding (KD = 0.25 nM).
(7) Triton X-100 lysates of labeled cells were immunoprecipitated with polyclonal antibodies prepared against purified human platelet GP IIb-IIIa complex.
(8) Regardless of the habitual diet, a test meal accentuated the rate of triacylglycerol appearance in whole plasma and in the very low density lipoproteins of Triton WR-1339-treated monkeys, and the rate of increase of the protein component after feeding was slightly higher.
(9) The Triton ghosts contracted immediately upon addition of ATP.
(10) The enzyme was removed from the cell envelope by treatment of the whole cells with sodium dodecyl sulfate, Triton X-100, sodium deoxycholate, and proteinase K.
(11) The protein appeared to form a dimer of molecular weight 65-70 000 in Triton X-100 solution and was shown to associate with both 125I-labelled and unlabelled rabbit transferrin to form a high molecular weight complex in the same solution.
(12) Serum gamma-GT was virtually unaffected by Triton X-100 at a concentration of 5% whereas urinary gamma-GT was 10-15% activated under similar conditions.
(13) For this, different detergents such as Triton X-100, CHAPS and n-octyl beta-D glucopyranoside were tested at various concentrations, durations and temperatures of incubation.
(14) PT painting resulted in rather higher sensitivity with Triton X-100 than with sodium lauryl sulphate.
(15) Temperature-dependent phase separation of infected synaptosomal and microsomal membranes with Triton X-114 yielded a phospholipid-rich phase containing a high concentration of PrP33-35Sc and greatest infectivity titers.
(16) Incubation of the platelets for intervals of 30 to 60 minutes with the microtubule-stabilizing agent taxol preserved the circumferential bundle after extraction with Triton X-100 even after washing five times.
(17) The P400 protein is purified by successive extraction of synaptosomal and microsomal membranes with 2% Triton X-100 and 25% Na-cholate and preparative gel electrophoresis in SDS.
(18) Biochemical analysis of the kinetics of assembly of two cytoplasmic plaque proteins of the desmosome, desmoplakins I (250,000 Mr) and II (215,000 Mr), in Madin-Darby canine kidney (MDCK) epithelial cells, demonstrated that these proteins exist in a soluble and insoluble pool, as defined by their extract ability in a Triton X-100 high salt buffer (CSK buffer).
(19) The effect of growth conditions on the extraction of cholesterol oxidase by Triton X-100 was investigated.
(20) All polypeptides migrate as one complex in a polyacrylamide gradient gel under non-denaturing conditions in the presence of 0.1% Triton X-100.