(n.) A proteolytic ferment, or enzyme, present in the pancreatic juice. Unlike the pepsin of the gastric juice, it acts in a neutral or alkaline fluid, and not only converts the albuminous matter of the food into soluble peptones, but also, in part, into leucin and tyrosin.
Example Sentences:
(1) This Mr 20,000 inhibitory activity was acid and heat stable and sensitive to dithiothreitol and trypsin.
(2) Under milder trypsin digestion conditions three resistant fragments were produced from the free protein.
(3) Treatment with trypsin gave essentially one radioactive peptide, the active site peptide, of approximately 2300 molecular weight.
(4) Benzyloxycarbonylarginine p-nitrophenyl ester and other activated esters of N-a-sustituted arginine salts may be useful reagents for introduction of trypsin-labile protecting groups into peptide fragments for purpose of polypeptide semi-synthesis.
(5) Trypsin had no effect on TGase activity when added to the cells 2 h after stimulation with a serum-containing medium.
(6) Amino acid analysis indicated a significant number of serine amino acids: N-terminal sequence data demonstrated a high level of homology; and trypsin digestion followed by reversed-phase HPLC indicated the possibility of multiple phosphorylation sites.
(7) Insulin incubation of plasma membranes pretreated with protease inhibitors (leupeptin, phenylmethylsulfonylfluoride) or with exogenous trypsin, but not chymotrypsin substrates (esters of arginine and tyrosine) yields an inactive supernatant on PDH.
(8) Conflicting reports exist on the postprandial response of serum cationic trypsin like immunoreactivity (SCTLI).
(9) The activation of calcium uptake by increasing concentrations of trypsin was paralleled by the reduction of phosphorylation of phospholamban.
(10) In the alpha 2M-thrombin, alpha 2M-plasmin, and alpha 2M-trypsin complexes, approximately 50%, 60%, and 75% of the subunits are cleaved, respectively.
(11) The presence or absence of 1,8-anilinonaphthalenesulfonic acid during labeling caused no change in the peptide maps of either lectin when digested with trypsin.
(12) Binding activity was labile to heat, and to treatment with pepsin or trypsin.
(13) Such gel was analyzed for its degree of substitution, and gels with three different degrees of substitution were used in chromatographic experiments with dextranase, alpha-amylase, lactate dehydrogenase, alpha-chymotrypsin and trypsin.
(14) Affinity-purified human placental ribonuclease inhibitor (PRI) was digested by trypsin.
(15) Gastrin-34-LI in antral extracts could be converted to gastrin-17-LI by trypsin in all species.
(16) Inhibitors of DC clustering, including trypsin, paraformaldehyde, and tunicamycin, abrogated the ability of DC to support antigen presentation and lectin-mediated proliferation.
(17) Optimal myocyte cultures were obtained using serial 0.2% crude trypsin digestions of hearts from 1-2-day-old rats.
(18) Limited trypsin digestion of purified platelet GPIIIa yielded a mixture of two-chain molecules comprised of an N-terminal fragment disulphide-bonded to one of four fragments, which began at residues 299, 303, 353 or 423.
(19) Antigenic properties of crystalline pepsin, trypsin and chymotrypsin were studied in 9 rabbits immunised with these enzymes.
(20) The cytoplasmic moiety of the inverted EII could be removed with trypsin without effecting the integrity of the liposomal membrane.
Trypsinogen
Definition:
(n.) The antecedent of trypsin, a substance which is contained in the cells of the pancreas and gives rise to the trypsin.
Example Sentences:
(1) Both trypsinogen and chymotrypsinogen content decreased after induction of pancreatitis, but there were no significant changes in the proenzyme contents in relation to injection-to-excision times.
(2) The results of experiments in which serum was fractionated by Sephadex G-200 gel filtration suggest that essentially all of the immunoreactive material in normal human serum is trypsinogen.
(3) Thus, in chronic alcoholics the secretory kinetics of trypsinogen and chymotrypsinogen were altered, but trypsin inhibitor secretion remained apparently unaffected.
(4) During the acute phase, it decreased more for trypsinogen I and chymotrypsinogen B than for amylase and lipase, whereas synthesis of the PAP increased dramatically.
(5) In the pancreas, several factors oppose trypsinogen autoactivation, whereas, in the duodenum, all the conditions are favorable for trypsinogen activation by enteropeptidase.
(6) No difference was observed between the streptozotocin pups and the control group in their pancreatic lipase and trypsinogen concentrations after 16 days.
(7) Tumors producing TATI often express tumor-associated trypsinogen.
(8) In the control children, lipase activity increased with advancing age, whereas trypsinogen showed no age-related trend.
(9) In the corpulent rat, both lipase- and chymotrypsinogen-specific activities and both the specific activities and the content of amylase or trypsinogen were lower than those of lean littermates.
(10) Chronic pancreatitis, although less well understood, is also associated with trypsinogen activation within the gland.
(11) These two membrane enzymes activated bovine pancreatic trypsinogen and had the same pH optima in the acid pH range.
(12) The relative rate of synthesis of one of the trypsinogens was unaffected 8 hours after RSD feeding, but was increased 16 hours after RSD feeding.
(13) Cholecystokinin caused a prompt increase in the concentration of both PSTI and trypsinogen.
(14) Both of these stimulators had a reduced effect on ribonuclease synthesis compared with amylase and trypsinogen synthesis but failed to increase myosin synthesis.
(15) Immunoreactive trypsinogen was measured in dried blood spots when the infants were 1 to 4 days old; if the level was elevated (greater than or equal to 140 micrograms per liter), the measurement was repeated (mean age, 38 days); if the level was again elevated, sweat testing was performed (mean age, 49 days).
(16) The rates of reaction of factor X, before and after activation, with the active-site titrant methanesulfonyl fluoride, suggest that the reactivity of the active-site serine residue in factor X is similar to that in trypsinogen and in factor Xa similar to that in trypsin.
(17) The trypsinogen and pancreatic isoamylase assays were also relatively nonspecific (specificity of 82.8% and 85.1%).
(18) Pancreatic trypsinogen and lipase content increased significantly more in younger animals, whereas amylase responses were not different between the two groups.
(19) Neither non-radioactive vitamin B12 nor non-pacreatic protein reduced the 57CoB12-uptake (p greater than 0.5 and p greater than 0.1) Crystalline trypsin and trypsinogen, but not chymotrypsin, also inhibited the uptake (p less than 0.05, p less than 0.02 and p greater than 0.05).
(20) Overall, 95.2 percent of the infants with cystic fibrosis (95 percent confidence interval, 85 to 99 percent) who did not have meconium ileus could be identified with the use of a trypsinogen cutoff level of 140 micrograms per liter on initial testing and 80 micrograms per liter on repeat testing.