What's the difference between trypsin and tryptone?
Trypsin
Definition:
(n.) A proteolytic ferment, or enzyme, present in the pancreatic juice. Unlike the pepsin of the gastric juice, it acts in a neutral or alkaline fluid, and not only converts the albuminous matter of the food into soluble peptones, but also, in part, into leucin and tyrosin.
Example Sentences:
(1) This Mr 20,000 inhibitory activity was acid and heat stable and sensitive to dithiothreitol and trypsin.
(2) Under milder trypsin digestion conditions three resistant fragments were produced from the free protein.
(3) Treatment with trypsin gave essentially one radioactive peptide, the active site peptide, of approximately 2300 molecular weight.
(4) Benzyloxycarbonylarginine p-nitrophenyl ester and other activated esters of N-a-sustituted arginine salts may be useful reagents for introduction of trypsin-labile protecting groups into peptide fragments for purpose of polypeptide semi-synthesis.
(5) Trypsin had no effect on TGase activity when added to the cells 2 h after stimulation with a serum-containing medium.
(6) Amino acid analysis indicated a significant number of serine amino acids: N-terminal sequence data demonstrated a high level of homology; and trypsin digestion followed by reversed-phase HPLC indicated the possibility of multiple phosphorylation sites.
(7) Insulin incubation of plasma membranes pretreated with protease inhibitors (leupeptin, phenylmethylsulfonylfluoride) or with exogenous trypsin, but not chymotrypsin substrates (esters of arginine and tyrosine) yields an inactive supernatant on PDH.
(8) Conflicting reports exist on the postprandial response of serum cationic trypsin like immunoreactivity (SCTLI).
(9) The activation of calcium uptake by increasing concentrations of trypsin was paralleled by the reduction of phosphorylation of phospholamban.
(10) In the alpha 2M-thrombin, alpha 2M-plasmin, and alpha 2M-trypsin complexes, approximately 50%, 60%, and 75% of the subunits are cleaved, respectively.
(11) The presence or absence of 1,8-anilinonaphthalenesulfonic acid during labeling caused no change in the peptide maps of either lectin when digested with trypsin.
(12) Binding activity was labile to heat, and to treatment with pepsin or trypsin.
(13) Such gel was analyzed for its degree of substitution, and gels with three different degrees of substitution were used in chromatographic experiments with dextranase, alpha-amylase, lactate dehydrogenase, alpha-chymotrypsin and trypsin.
(14) Affinity-purified human placental ribonuclease inhibitor (PRI) was digested by trypsin.
(15) Gastrin-34-LI in antral extracts could be converted to gastrin-17-LI by trypsin in all species.
(16) Inhibitors of DC clustering, including trypsin, paraformaldehyde, and tunicamycin, abrogated the ability of DC to support antigen presentation and lectin-mediated proliferation.
(17) Optimal myocyte cultures were obtained using serial 0.2% crude trypsin digestions of hearts from 1-2-day-old rats.
(18) Limited trypsin digestion of purified platelet GPIIIa yielded a mixture of two-chain molecules comprised of an N-terminal fragment disulphide-bonded to one of four fragments, which began at residues 299, 303, 353 or 423.
(19) Antigenic properties of crystalline pepsin, trypsin and chymotrypsin were studied in 9 rabbits immunised with these enzymes.
(20) The cytoplasmic moiety of the inverted EII could be removed with trypsin without effecting the integrity of the liposomal membrane.
Tryptone
Definition:
(n.) The peptone formed by pancreatic digestion; -- so called because it is formed through the agency of the ferment trypsin.
Example Sentences:
(1) Counts of E. coli were also higher on mFC agar (without rosalic acid) after a period of resuscitation on tryptone-soy agar supplemented with 0.1% yeast extract than on a 4% Teepol-lactose medium.
(2) Neuraminidase inductions were primarily studied with tryptone-yeast extract-grown cells after washing and resuspension in a defined replacement medium.
(3) Cells growing in yeast extract-tryptone-sodium chloride medium (LB broth) were lysed upon the addition of lactose.
(4) McBride Listeria agar (MLA), gum base nalidixic acid tryptone soy agar (GBNTSA), modified Despierres agar (MDA), and modified MLA (MMLA) performed best for recovering all inoculum populations from milk and ice cream mix.
(5) Supplementing NB with tryptone or casein reduced the toxicity of cocoa.
(6) Modified Despierres agar and modified McBride Listeria agar yielded poorer recovery of heat-injured cells than did McBride Listeria agar and gum base-nalidixic acid-tryptone soya agar.
(7) Growth on glutamate in the dark took place without an initial lag phase, whereas growth on tryptone began only after a 2-day lag phase that could be abolished by the simultaneous presence of glucose.
(8) Such media will be quite favourable for certain culturing problems, but complex substrate (yeast extract, peptone, tryptone) should be added for high yields.
(9) The sources which mostly supported the biosynthesis of the inhibitors were tryptone (3%) for TI-1, and peptone (3%) for CI-1 respectively.
(10) A medium based upon tryptone, yeast extract, cystine (TYC) agar and incorporating bacitracin and sucrose has been evaluated for selective isolation of Streptococcus mutans.
(11) Growth of the isolates in an acidified tryptone-yeast extract medium was accompanied by a rise in the pH of the medium from 3.5 to above 7.0 Ammonia production, as indicated by Nesslerization, was associated with the deamination of glutamic and aspartic acids.
(12) The extracellular growth products of Micropolyspora faeni, Thermoactinomyces vulgaris and Thermoactinomyces candidus were prepared by three methods; (1) double dialysis; (2) growth in tryptone soya broth (TSB) and (3) synthetic broth (AOAC).
(13) Using ion-exchange columns, humic acid and the amino acids of tryptone were found to be anionic and cationic in water (pH 7.0), respectively.
(14) Ability of flush without disinfectant to clear sets of contamination was analyzed by collecting multiple samples at each step of the procedure, enriching with tryptone broth and verifying bacterial growth.
(15) Soluble organics, such as tryptone, yeast extract, cysteine, succinic acid, and increasing concentrations of neopeptone, also reduced the toxicity of Pb.
(16) Thus, heat damage was repaired in tryptone broth but not on tryptone agar.
(17) In contrast to the repression observed when cells were grown on glucose, sucrose, or fructose, system II was not repressed by growth on tryptone and casein hydrolysate.
(18) The inoculation of Aspergillus flavus spores into a culture of Streptococcus lactis in Lablemco tryptone broth medium resulted in little or no aflatoxin accumulation even though the growth of the fungus was not hindered.
(19) The necessity to transfer membranes from the non-selective medium tryptone soy agar (TSA) to the selective medium tryptone bile agar (TBA) after pre-incubation for 4 h was considered disadvantageous for practical purposes.
(20) In tryptone broth, recA or polA mutants also recovered but a lex mutant did not.