(1) Initially, all attempts to unknot or remove the catheter failed.
(2) "Well…" His delightful press secretary, Lena, starts giggling as her boss tries to unknot himself from this contradiction.
(3) The purpose of this study was to identify possible mechanisms of resistance present in these cells by using human mdr1 and topoisomerase II DNA probes, antibodies to these gene products, and P4 phage unknotting assay for topoisomerase II activities.
(4) The extractable topoisomerase II activity of Bloom's syndrome fibroblasts, as measured by unknotting of page P4 DNA, is much more strongly inhibited by cell growth in medium containing BrdU than is that of normal fibroblasts.
(5) In the present study, we found that nuclear extracts of these sublines (approximately 50- and approximately 140-fold resistant to VM-26) required 2 and 8 times more adenosine 5'-triphosphate (ATP), respectively, to achieve half-maximal stimulation of unknotting activity compared to extracts from the sensitive cells.
(6) In the presence of 500 microM ATP, equimolar concentrations of 2-bromo-dATP, dideoxy-ATP, 2-chloro-dATP, 2-fluoro-dATP, and dideoxy-GTP nucleotide analogues inhibited the unknotting activity of the Topo II enzyme.
(7) Sensitivity of the topo II unknotting assay to inhibition by VP-16 was the same for the parent and all resistant cell lines.
(8) The phage P4-unknotting activity of nuclear extracts decreased 2-fold when AA8 cells entered into the non-cycling state, but there was no difference in sensitivity to amsacrine between log- and plateau-phase nuclear extracts.
(9) Methods are described for accurate measurement of 10-100 ng of unknotted P4 DNA.
(10) Our preliminary results demonstrate remarkable activity of Topo II with specific conversion of knotted form P4 DNA to unknotted form in human kidney cancer cells, YCR and ACHN.
(11) The conditions for the reactions of relaxation, unknotting, decatenation, and catenation were found to be similar to those observed with enzymes from other eukaryotic cells.
(12) The ATP concentration required for equivalent activity in a DNA-unknotting assay is 2- to 8-fold higher in nuclear extracts from drug-resistant cell lines as compared with the parental line.
(13) Several quinolones and antitumor compounds were tested as inhibitors of purified calf thymus topoisomerase II in unknotting, catenation, radiolabeled DNA cleavage, and quantitative nonradiolabeled cleavage assays.
(14) This method check relative Topo II activity with its conversion of knotted form P4 phage DNA to unknotted form.
(15) Regular chromic material was stronger in unknotted tensile strength and fracture load for all gauges.
(16) P4 unknotting assays of the purified nuclear type II topoisomerase in the presence of m-AMSA and VM-26 indicated that the mitochondrial and nuclear enzymes behaved similarly, although the mitochondrial enzyme appeared to be inhibited more strongly.
(17) More than 99% of the inversion products were unknotted rings.
(18) The enzyme can catenate supercoiled DNA molecules, unknot DNA, and cleave double-stranded DNA.
(19) Topoisomerase II activity in nuclear extracts, as determined by the P4 phage DNA-unknotting assay, was more easily detected and measured at lower NaCl concentrations in NCI-H69 than in NCI-H187 cells.
(20) The level of DNA topoisomerase II protein, detected by antibody staining, decreased concomitantly with a general decrease in DNA topoisomerase II unknotting activity, while DNA topoisomerase I activity was not affected.
Unknow
Definition:
(v. t.) To cease to know; to lose the knowledge of.
(v. t.) To fail of knowing; to be ignorant of.
(a.) Unknown.
Example Sentences:
(1) Still, even as unknowable as this decision may be for him, as any decision is, really, he is far more qualified to understand his desires and goals that would inform that decision than anyone else is.
(2) Parents unknowingly adjust the structure and dynamics of speech to the constraints of infant capacities, detach prosodic musicality from lexical structure, and use it in particularly expressive forms for the delivery of the first prototypical messages.
(3) This form of mechanical purpura, often caused by suction may be deliberately or unknowingly induced by the patient.
(4) The results indicate that one group of surgeons (34% of those responding) believes each type of surgery has equal survival rates but unknowingly influences the patient to choose modified radical mastectomy, with a subtly biased presentation.
(5) These counter-transferential concerns ultimately made the woman's psychological essence an unknowable riddle for Freud.
(6) The unknown, on the other hand, is not just unknown but perhaps even unknowable – and its consequences could be worse.
(7) The other women were unknowingly pregnant at the time of the sterilization.
(8) While Europeans cannot unknowingly run up a bill larger than €50 (£42) while abroad, roaming charges are still many times higher than domestic call costs and will remain so without further legislation.
(9) The future threats can remain fully unknowable and fully addressable – on the individual level.
(10) He ended his life as unknowable and contrary as the 22-year-old who made Do the Ostrich.
(11) One of the ideas of the book is about the unknowability or uncategorisability of human behaviour, and I was rather tempted into those ambiguous sexual areas."
(12) You could [attack] your factional enemies to your heart’s content and that would be a dangerous system.” Simplification of the complex system with stronger definitions The opacity of the current entitlements system often leads to politicians knowingly or unknowingly exploiting the grey areas.
(13) "Judging additionality has turned out to be unknowable and unworkable.
(14) The resistance was not due to the presence of any inhibitor in the crude extract, but possibly due to the special configuration of the lectin molecule or other unknow reasons.
(15) During the suboccipital (retrosigmoid) removal of a tumor, the surgeon unknowingly may leave tumor remnants leading to regrowth.
(16) Obama meant to call attention to Trayvon Martin's unknowable potential (how many future presidents, future Nobel prize winners, future mothers and fathers have we lost to pointless racial violence?)
(17) Given diplomacy's ineffectiveness and the unknowable but terrible consequences of air strikes, it is easy to see why covert action is the least bad option; most of the successes and failures in this war will remain unsung, but some have made news.
(18) If the eye were unknowingly anesthetized, exposure to an irritant could go undected and cause injury.
(19) The metabolism of americium-241 has been studied during an 8-year period in an adult male and his son who, at the ages of 50 and 4 years, respectively, were accidentally and unknowingly contaminated within their home by means of inhalation.
(20) The process, of balancing emotional and reproductive needs against the instinct for survival, and the categorisation of an essentially unknowable risk, is so complicated that counsellors and charities have developed a “risk tool” to help women judge their own needs.