(1) Initially, all attempts to unknot or remove the catheter failed.
(2) "Well…" His delightful press secretary, Lena, starts giggling as her boss tries to unknot himself from this contradiction.
(3) The purpose of this study was to identify possible mechanisms of resistance present in these cells by using human mdr1 and topoisomerase II DNA probes, antibodies to these gene products, and P4 phage unknotting assay for topoisomerase II activities.
(4) The extractable topoisomerase II activity of Bloom's syndrome fibroblasts, as measured by unknotting of page P4 DNA, is much more strongly inhibited by cell growth in medium containing BrdU than is that of normal fibroblasts.
(5) In the present study, we found that nuclear extracts of these sublines (approximately 50- and approximately 140-fold resistant to VM-26) required 2 and 8 times more adenosine 5'-triphosphate (ATP), respectively, to achieve half-maximal stimulation of unknotting activity compared to extracts from the sensitive cells.
(6) In the presence of 500 microM ATP, equimolar concentrations of 2-bromo-dATP, dideoxy-ATP, 2-chloro-dATP, 2-fluoro-dATP, and dideoxy-GTP nucleotide analogues inhibited the unknotting activity of the Topo II enzyme.
(7) Sensitivity of the topo II unknotting assay to inhibition by VP-16 was the same for the parent and all resistant cell lines.
(8) The phage P4-unknotting activity of nuclear extracts decreased 2-fold when AA8 cells entered into the non-cycling state, but there was no difference in sensitivity to amsacrine between log- and plateau-phase nuclear extracts.
(9) Methods are described for accurate measurement of 10-100 ng of unknotted P4 DNA.
(10) Our preliminary results demonstrate remarkable activity of Topo II with specific conversion of knotted form P4 DNA to unknotted form in human kidney cancer cells, YCR and ACHN.
(11) The conditions for the reactions of relaxation, unknotting, decatenation, and catenation were found to be similar to those observed with enzymes from other eukaryotic cells.
(12) The ATP concentration required for equivalent activity in a DNA-unknotting assay is 2- to 8-fold higher in nuclear extracts from drug-resistant cell lines as compared with the parental line.
(13) Several quinolones and antitumor compounds were tested as inhibitors of purified calf thymus topoisomerase II in unknotting, catenation, radiolabeled DNA cleavage, and quantitative nonradiolabeled cleavage assays.
(14) This method check relative Topo II activity with its conversion of knotted form P4 phage DNA to unknotted form.
(15) Regular chromic material was stronger in unknotted tensile strength and fracture load for all gauges.
(16) P4 unknotting assays of the purified nuclear type II topoisomerase in the presence of m-AMSA and VM-26 indicated that the mitochondrial and nuclear enzymes behaved similarly, although the mitochondrial enzyme appeared to be inhibited more strongly.
(17) More than 99% of the inversion products were unknotted rings.
(18) The enzyme can catenate supercoiled DNA molecules, unknot DNA, and cleave double-stranded DNA.
(19) Topoisomerase II activity in nuclear extracts, as determined by the P4 phage DNA-unknotting assay, was more easily detected and measured at lower NaCl concentrations in NCI-H69 than in NCI-H187 cells.
(20) The level of DNA topoisomerase II protein, detected by antibody staining, decreased concomitantly with a general decrease in DNA topoisomerase II unknotting activity, while DNA topoisomerase I activity was not affected.
Unlink
Definition:
(v. t.) To separate or undo, as links; to uncoil; to unfasten.
Example Sentences:
(1) A cluster of unlinked genes encoding gluconeogenic enzymes in the mouse is characterized by the failure of normal hormone-inducible expression in animals homozygous for one of several overlapping deletions mapping on chromosome 7 near the albino locus.
(2) Four of the mutants had no effect on chylous ascites, but two mutants linked with ragged, and one unlinked, showed a complex situation involving enhancement, inhibition, epistacy and other interactions.
(3) At least two unlinked mutations were associated with such phenotypes.
(4) Strains with derepressed levels of the isoleucine and valine biosynthetic enzymes owing to linked or unlinked genetic lesions were found to exhibit ilv messenger RNA levels from 1.5- to 4-fold higher than did their isogenic parents.
(5) Several overlapping chromosomal deletions spanning the albino locus in the mouse cause perinatal lethality when homozygous and a block in the transcriptional induction of various unlinked hepatocyte-specific genes.
(6) The phenotypic expression of resistance at the fpaA locus can be suppressed by mutation of the str locus from str-s to str-r, whereas that at an unlinked fpa locus cannot.
(7) This inference is supported by the observation that a significant interaction (i.e., epistasis) was detected between the QTL(s) in the conserved region and an unlinked RFLP marker locus in both species.
(8) b) Locus TSM1 appears to be weakly linked to the locus PAR1 and to the loci RIB1 and RIB3 but unlinked to the locus OLI1.
(9) The three electrophoretic variants of phosphoglucomutase in Saccharomyces cerevisiae breeding stocks are produced by two unlinked genes, pgm-1 and pgm-2; pgm-1 contains two known alleles, pgm-1a and pgm-1b, each of which specifies a minor phosphoglucomutase component, and pgm-2 specifies the major phosphoglucomutase component.
(10) The "preferred" model specifying interaction between two major unlinked autosomal loci was confirmed indirectly by further breeding tests.
(11) The enzyme is an aggregate consisting of two protein components, coded for by the unlinked genes cpaI and cpaII.
(12) The second site is on chromosome III, unlinked to the centromere and distal to the mating type locus.
(13) Since in this study all unlinked genes segregated independently, this is direct evidence that MCF viruses participate in the induction of erythroleukemia.
(14) The segregation data indicate that the AKR mouse contains two unlinked, autosomal, chromosomal loci, either of which suffices to induce detectable levels of infectious virus in Fv-1(n) progeny by 6 wk of age.
(15) Additionally, we examined the combined data for evidence that a third, as yet unlinked locus exists.
(16) It is known that glnR5, a regulatory mutation unlinked to glnS, causes overproduction of glutaminyl-tRNA synthetase.
(17) relA and spoT are designations for two unlinked Escherichia coli genes whose products function in the synthesis and degradation of guanosine 3',5'-bispyrophosphate during the stringent regulatory response to amino acid deprivation.
(18) Allosuppressor (sal) mutations enhance the efficiency of the yeast ochre suppressor SUQ5 and define five unlinked loci, SAL1-SAL5.
(20) These data demonstrate that two unlinked transforming regions of human cytomegalovirus can cooperate to produce an aggressive tumorigenic phenotype.