(1) Initially, all attempts to unknot or remove the catheter failed.
(2) "Well…" His delightful press secretary, Lena, starts giggling as her boss tries to unknot himself from this contradiction.
(3) The purpose of this study was to identify possible mechanisms of resistance present in these cells by using human mdr1 and topoisomerase II DNA probes, antibodies to these gene products, and P4 phage unknotting assay for topoisomerase II activities.
(4) The extractable topoisomerase II activity of Bloom's syndrome fibroblasts, as measured by unknotting of page P4 DNA, is much more strongly inhibited by cell growth in medium containing BrdU than is that of normal fibroblasts.
(5) In the present study, we found that nuclear extracts of these sublines (approximately 50- and approximately 140-fold resistant to VM-26) required 2 and 8 times more adenosine 5'-triphosphate (ATP), respectively, to achieve half-maximal stimulation of unknotting activity compared to extracts from the sensitive cells.
(6) In the presence of 500 microM ATP, equimolar concentrations of 2-bromo-dATP, dideoxy-ATP, 2-chloro-dATP, 2-fluoro-dATP, and dideoxy-GTP nucleotide analogues inhibited the unknotting activity of the Topo II enzyme.
(7) Sensitivity of the topo II unknotting assay to inhibition by VP-16 was the same for the parent and all resistant cell lines.
(8) The phage P4-unknotting activity of nuclear extracts decreased 2-fold when AA8 cells entered into the non-cycling state, but there was no difference in sensitivity to amsacrine between log- and plateau-phase nuclear extracts.
(9) Methods are described for accurate measurement of 10-100 ng of unknotted P4 DNA.
(10) Our preliminary results demonstrate remarkable activity of Topo II with specific conversion of knotted form P4 DNA to unknotted form in human kidney cancer cells, YCR and ACHN.
(11) The conditions for the reactions of relaxation, unknotting, decatenation, and catenation were found to be similar to those observed with enzymes from other eukaryotic cells.
(12) The ATP concentration required for equivalent activity in a DNA-unknotting assay is 2- to 8-fold higher in nuclear extracts from drug-resistant cell lines as compared with the parental line.
(13) Several quinolones and antitumor compounds were tested as inhibitors of purified calf thymus topoisomerase II in unknotting, catenation, radiolabeled DNA cleavage, and quantitative nonradiolabeled cleavage assays.
(14) This method check relative Topo II activity with its conversion of knotted form P4 phage DNA to unknotted form.
(15) Regular chromic material was stronger in unknotted tensile strength and fracture load for all gauges.
(16) P4 unknotting assays of the purified nuclear type II topoisomerase in the presence of m-AMSA and VM-26 indicated that the mitochondrial and nuclear enzymes behaved similarly, although the mitochondrial enzyme appeared to be inhibited more strongly.
(17) More than 99% of the inversion products were unknotted rings.
(18) The enzyme can catenate supercoiled DNA molecules, unknot DNA, and cleave double-stranded DNA.
(19) Topoisomerase II activity in nuclear extracts, as determined by the P4 phage DNA-unknotting assay, was more easily detected and measured at lower NaCl concentrations in NCI-H69 than in NCI-H187 cells.
(20) The level of DNA topoisomerase II protein, detected by antibody staining, decreased concomitantly with a general decrease in DNA topoisomerase II unknotting activity, while DNA topoisomerase I activity was not affected.
Unravel
Definition:
(v. t.) To disentangle; to disengage or separate the threads of; as, to unravel a stocking.
(v. t.) Hence, to clear from complication or difficulty; to unfold; to solve; as, to unravel a plot.
(v. t.) To separate the connected or united parts of; to throw into disorder; to confuse.
(v. i.) To become unraveled, in any sense.
Example Sentences:
(1) However, as the plan unravels, Professor Marcus's team turn on one another, with painfully (if painfully funny) results.
(2) Her black persona unravelled this week when Ruthanne and Larry Dolezal, a couple named on her Montana birth certificate as her biological parents, told Spokane’s KREM 2 News that her ancestry was German and Czech, with traces of Native American.
(3) Putting all this information together we can begin to unravel the problem of how the Listeria forms the cytoskeleton and what is the biological purpose of this tail.
(4) Thus, 14.7K appears to be a general inhibitor of TNF cytolysis, and as such should be an important tool in unraveling the mechanism of TNF cytolysis.
(5) This communication reviews the almost 40 years of studies by Jack Metcoff, MD, and coworkers to unravel the causes of fetal malnutrition and their efforts to prevent it.
(6) Because the housing crisis goes far beyond us Focus E15 mums | Jasmin Stone Read more Annette May, 68, from Lambeth Annette May has watched with mounting dismay as the community fabric of the council estate where she has lived for 44 years steadily unravels.
(7) Substantial progress has been made in unraveling the organization of the circadian system of Aplysia californica.
(8) The free ends of the microtubules appear unraveled; they are seen first as single elements, then as doublets, and finally are arranged into a cylinder.
(9) Unraveled filaments reconstituted from NF-L plus either NF-M or NF-H indicated that NF-M and NF-H are incorporated evenly into each protofibril.
(10) Athens was unravelling into chaos, unable to form a government and forced into fresh elections , plunging the markets into freefall as Europe's leaders abandoned any pretence that a Greek exit from the euro might not be imminent.
(11) The chancellor leaves the Treasury trying to hide the cost of his mistakes while his reputation for economic competence continues to unravel."
(12) With the eurozone unravelling and world markets in turmoil, threatening even the meagre recovery the UK economy had achieved since the onset of the credit crunch, he repeatedly evokes a mood of national emergency to explain why the coalition he forged with David Cameron is the right government for the times.
(13) Without a rescue, president Nicos Anastasiades said Cyprus would default and threaten to unravel investor confidence in the eurozone.
(14) This section was memorably captured by the computer and security expert Caspar Bowden , who wrote: "Interpreting that section requires the unravelling of a triple-nested inversion of meanings across six cross-referenced subsections, linked to a dozen other cross-linked definitions, which are all dependent on a highly ambiguous 'notwithstanding'."
(15) If the statistics aren't right the whole story, beautiful as it is, unravels," he said.
(16) But sometimes a smile is not enough.” As the latest proposed deal to avoid Greece’s bankruptcy threatens to unravel , a row is raging on Rhodes and several other Greek islands over fears that they are being unfairly targeted.
(17) George Osborne’s claim that the government secured a major corporation tax deal with Google appear to be unravelling after it emerged that a quarter of the £130m recovered by HM Revenue & Customs related to the US company’s share options scheme.
(18) I have lived in Greece my whole life and experienced the economic crisis as it unraveled the past years.
(19) As the field of human genetics successfully continues to unravel the secrets of an individual's genetic makeup, the social processes of stigmatization and ostracism of those with "undesirable" traits have the potential to increase.
(20) There's got to be a deal here between the taxpayer and the scheme member and that deal is going to unravel if the people in the public sector say they will not contemplate change."