(1) Initially, all attempts to unknot or remove the catheter failed.
(2) "Well…" His delightful press secretary, Lena, starts giggling as her boss tries to unknot himself from this contradiction.
(3) The purpose of this study was to identify possible mechanisms of resistance present in these cells by using human mdr1 and topoisomerase II DNA probes, antibodies to these gene products, and P4 phage unknotting assay for topoisomerase II activities.
(4) The extractable topoisomerase II activity of Bloom's syndrome fibroblasts, as measured by unknotting of page P4 DNA, is much more strongly inhibited by cell growth in medium containing BrdU than is that of normal fibroblasts.
(5) In the present study, we found that nuclear extracts of these sublines (approximately 50- and approximately 140-fold resistant to VM-26) required 2 and 8 times more adenosine 5'-triphosphate (ATP), respectively, to achieve half-maximal stimulation of unknotting activity compared to extracts from the sensitive cells.
(6) In the presence of 500 microM ATP, equimolar concentrations of 2-bromo-dATP, dideoxy-ATP, 2-chloro-dATP, 2-fluoro-dATP, and dideoxy-GTP nucleotide analogues inhibited the unknotting activity of the Topo II enzyme.
(7) Sensitivity of the topo II unknotting assay to inhibition by VP-16 was the same for the parent and all resistant cell lines.
(8) The phage P4-unknotting activity of nuclear extracts decreased 2-fold when AA8 cells entered into the non-cycling state, but there was no difference in sensitivity to amsacrine between log- and plateau-phase nuclear extracts.
(9) Methods are described for accurate measurement of 10-100 ng of unknotted P4 DNA.
(10) Our preliminary results demonstrate remarkable activity of Topo II with specific conversion of knotted form P4 DNA to unknotted form in human kidney cancer cells, YCR and ACHN.
(11) The conditions for the reactions of relaxation, unknotting, decatenation, and catenation were found to be similar to those observed with enzymes from other eukaryotic cells.
(12) The ATP concentration required for equivalent activity in a DNA-unknotting assay is 2- to 8-fold higher in nuclear extracts from drug-resistant cell lines as compared with the parental line.
(13) Several quinolones and antitumor compounds were tested as inhibitors of purified calf thymus topoisomerase II in unknotting, catenation, radiolabeled DNA cleavage, and quantitative nonradiolabeled cleavage assays.
(14) This method check relative Topo II activity with its conversion of knotted form P4 phage DNA to unknotted form.
(15) Regular chromic material was stronger in unknotted tensile strength and fracture load for all gauges.
(16) P4 unknotting assays of the purified nuclear type II topoisomerase in the presence of m-AMSA and VM-26 indicated that the mitochondrial and nuclear enzymes behaved similarly, although the mitochondrial enzyme appeared to be inhibited more strongly.
(17) More than 99% of the inversion products were unknotted rings.
(18) The enzyme can catenate supercoiled DNA molecules, unknot DNA, and cleave double-stranded DNA.
(19) Topoisomerase II activity in nuclear extracts, as determined by the P4 phage DNA-unknotting assay, was more easily detected and measured at lower NaCl concentrations in NCI-H69 than in NCI-H187 cells.
(20) The level of DNA topoisomerase II protein, detected by antibody staining, decreased concomitantly with a general decrease in DNA topoisomerase II unknotting activity, while DNA topoisomerase I activity was not affected.
Untangle
Definition:
(v. t.) To loose from tangles or intricacy; to disentangle; to resolve; as, to untangle thread.
Example Sentences:
(1) Loose ends in efforts to untangle the Gordian knot of Syria | Letters Read more “What is important is Russia has to not be engaged in any activities against anybody but [Isis],” secretary of state John Kerry said.
(2) Loose ends in efforts to untangle the Gordian knot of Syria | Letters Read more Moscow, however, angrily dismissed the reports as false, the TASS news agency reported.
(3) Andrew Romano, Newsweek How would these eloquent know-it-alls – these brainiacs bent on "speaking truth to stupid" – untangle the knotty threads of information that make actual breaking news so difficult to sort out?
(4) The Apple-Samsung case has so far lasted for four weeks, and the jurors are expected to deliberate for another week as they try to untangle the complex forms – in which they have to decide, among other things, whether any of 21 different Samsung tablets and smartphones infringed any of 10 different patents on functionality – such as the "rubber band" effect when trying to scroll past the top of a list – and whether the "trade dress" of Apple's products is sufficiently "famous" to merit protection.
(5) Such agreement to begin reining in respective proxies is necessary in the process of untangling the mess of foreign interests that has been tearing Syria apart for the last five years.
(6) Dawn Foster Contributing editor, Guardian housing network Central government: untangling British and European laws could cause years of instability Amid the uncertainty around what a UK vote to leave the EU could mean for central government, one thing is clear.
(7) The objective of the current study was to untangle the effects of a number of variables likely to affect autonomic activation during human speech.
(8) In the abscence of a priori knowledge of the functional form, it is difficult to untangle true effects from spurious ones.
(9) A world where cycling could become so safe it was actually relaxing again, and traffic jams untangled themselves, and pollution didn’t choke cities to death after all.
(10) "Polygraph was an act of mourning, a way to untangle very, very dark feelings about that event," Lepage says.
(11) Bond covertly enlists Moneypenny ( Naomie Harris ) and Q ( Ben Whishaw ) to help him seek out Madeleine Swann (Léa Seydoux), the daughter of his old nemesis Mr White (Jesper Christensen), who may hold the clue to untangling the web of SPECTRE.
(12) Beyond SW1, it is hoped that businesses will volunteer to untangle themselves from the old boy net.
(13) I was faced with a labyrinth of political, religious and cultural elements that from the outside seemed impossible to understand much less untangle.
(14) Here’s a three-point plan to untangle it | Andrew Allen Read more While Southern’s plans for onboard supervisors have been approved by the independent rail safety body, the RMT general secretary, Mick Cash, told the transport select committee that there were genuine safety concerns over making drivers responsible for closing the doors on crowded platforms, and that there were insufficient guarantees over future staffing of trains.
(15) Seabirds are one of most threatened groups of birds in the world, but untangling the effects of multiple threats to seabirds is a challenge, one which underlines the importance of ongoing research such as this which helps determine vulnerability of different species.” The findings are published in the journal Science Advances.
(16) I had plenty of fashion cupboard experience, but to them I was new so my first task was to untangle a massive pile of coat hangers.
(17) However, while clinical observations of human behavior are particularly relevant, it is difficult to untangle confounding factors and thus determine unequivocal causal relationships.
(18) The move is part of President Pedro Pablo Kuczynski’s bid to “untangle” stalled investments from red tape.
(19) The weather had cleared and Machado was back on the roof, untangling wire, when Bumba’s summit suddenly cratered out.
(20) Ellis is very well-informed about the complexities of disability policy, after spending thousands of hours untangling refused benefit applications, and attending hundreds of tribunals with clients, fighting for decisions not to award disability benefit to be overturned.