(1) Initially, all attempts to unknot or remove the catheter failed.
(2) "Well…" His delightful press secretary, Lena, starts giggling as her boss tries to unknot himself from this contradiction.
(3) The purpose of this study was to identify possible mechanisms of resistance present in these cells by using human mdr1 and topoisomerase II DNA probes, antibodies to these gene products, and P4 phage unknotting assay for topoisomerase II activities.
(4) The extractable topoisomerase II activity of Bloom's syndrome fibroblasts, as measured by unknotting of page P4 DNA, is much more strongly inhibited by cell growth in medium containing BrdU than is that of normal fibroblasts.
(5) In the present study, we found that nuclear extracts of these sublines (approximately 50- and approximately 140-fold resistant to VM-26) required 2 and 8 times more adenosine 5'-triphosphate (ATP), respectively, to achieve half-maximal stimulation of unknotting activity compared to extracts from the sensitive cells.
(6) In the presence of 500 microM ATP, equimolar concentrations of 2-bromo-dATP, dideoxy-ATP, 2-chloro-dATP, 2-fluoro-dATP, and dideoxy-GTP nucleotide analogues inhibited the unknotting activity of the Topo II enzyme.
(7) Sensitivity of the topo II unknotting assay to inhibition by VP-16 was the same for the parent and all resistant cell lines.
(8) The phage P4-unknotting activity of nuclear extracts decreased 2-fold when AA8 cells entered into the non-cycling state, but there was no difference in sensitivity to amsacrine between log- and plateau-phase nuclear extracts.
(9) Methods are described for accurate measurement of 10-100 ng of unknotted P4 DNA.
(10) Our preliminary results demonstrate remarkable activity of Topo II with specific conversion of knotted form P4 DNA to unknotted form in human kidney cancer cells, YCR and ACHN.
(11) The conditions for the reactions of relaxation, unknotting, decatenation, and catenation were found to be similar to those observed with enzymes from other eukaryotic cells.
(12) The ATP concentration required for equivalent activity in a DNA-unknotting assay is 2- to 8-fold higher in nuclear extracts from drug-resistant cell lines as compared with the parental line.
(13) Several quinolones and antitumor compounds were tested as inhibitors of purified calf thymus topoisomerase II in unknotting, catenation, radiolabeled DNA cleavage, and quantitative nonradiolabeled cleavage assays.
(14) This method check relative Topo II activity with its conversion of knotted form P4 phage DNA to unknotted form.
(15) Regular chromic material was stronger in unknotted tensile strength and fracture load for all gauges.
(16) P4 unknotting assays of the purified nuclear type II topoisomerase in the presence of m-AMSA and VM-26 indicated that the mitochondrial and nuclear enzymes behaved similarly, although the mitochondrial enzyme appeared to be inhibited more strongly.
(17) More than 99% of the inversion products were unknotted rings.
(18) The enzyme can catenate supercoiled DNA molecules, unknot DNA, and cleave double-stranded DNA.
(19) Topoisomerase II activity in nuclear extracts, as determined by the P4 phage DNA-unknotting assay, was more easily detected and measured at lower NaCl concentrations in NCI-H69 than in NCI-H187 cells.
(20) The level of DNA topoisomerase II protein, detected by antibody staining, decreased concomitantly with a general decrease in DNA topoisomerase II unknotting activity, while DNA topoisomerase I activity was not affected.
Untie
Definition:
(v. t.) To loosen, as something interlaced or knotted; to disengage the parts of; as, to untie a knot.
(v. t.) To free from fastening or from restraint; to let loose; to unbind.
(v. t.) To resolve; to unfold; to clear.
(v. i.) To become untied or loosed.
Example Sentences:
(1) We're simply untying heads' hands so they can spend the money as they see fit.
(2) Eight of 9 Mute swans (Cygnus olor) untied in the river acrossing the central part of Tottori-city died within the period of 40 days of summer in 1989.
(3) 1.28am BST Heat 15-20 Spurs, 3:53 remaining in 1st quarter Tony Parker sneaks through two defenders to untie it.
(4) LD may be used to follow the complexation both stoichmetrically and structurally, since when specified to unti complex concentration LD provides a measure of the average orientation of the absorbing transition dipole.
(5) The donor heart is transplanted heterotopically into the recipient with the brachiocephalic artery anastomosed to the renal artery with 10 single sutures, and the pulmonary artery to the renal vein with 2 continuous, semicircular sutures which were left untied.
(6) In both experiments, bonding to each other of both tied and untied specimens was observed one month after implantation.
(7) The EU changed its food aid policy in 1996, shifting to cash donations, and Canada fully untied its food aid budget in 2008 – a move commended internationally, including by the Organisation for Economic Co-operation and Development.
(8) The holding power of many of the knots that untied was substantially less than that of knots reaching knot break.
(9) On the 20th realimentation day, the absolute red cell volume had increased less than the absolute plasma volume, the total blood volume was almost normal per unti of body size.
(10) In our series of 42 patients who underwent adjustable-suture surgery, 22 cases were not adjusted and 30 cases were left untied.
(11) When one of the soldiers wanted to have sex with one of us, he would come and untie us, take us away then bring us back to tie up,” said Nyabol, shaking at the memory.
(12) Most other donors have "untied" their food aid budgets and have shifted towards buying food closer to where it is needed, on the basis that it is cheaper, faster and easier to find food local people are used to eating.
(13) Furthermore, no knot has come untied or developed other complications.
(14) By passing a spring guidewire into the catheter under fluoroscopic control, the knot was easily untied leaving the catheter correctly in place.
(15) An unrelated second pair of repeat sequences was located at 0.67 and 0.88 map untis.
(16) They called for a scaling up of aid commitments, for concrete timetables for reaching the commitments, and for improving the quality of aid, including full untying of aid (lifting requirements by some donors that aid be spent on goods and services provided by companies based in their own countries, or a limited number of countries).
(17) Multilaterals benefit from having greater independence from immediate political considerations; their aid is untied and much less fragmented, and their larger projects reduce the administrative burden on recipients.
(18) The affair did leave some positive legacies: a cross-party consensus that aid should be officially "untied" from commercial interests, a new act enshrining in law its poverty reduction focus, and a cabinet minister for the new Department for International Development (DfID).
(19) Standing with the cheering crowds by the finish line on Monday, Liliana’s mother Nancy said that only “little things” – a red traffic light, an untied shoelace – prevented them from being right where Richard was killed that day.
(20) the heat quantity generated by the tumour per untis of volume and time, computed from from intramammary temperature and thermal conductivity measurements made using of fluvographic needle probes), is typical of each cancer and re7ains remarkably constant during the growth in spite of themorphological and of the morphological and circulatory changes; b) the tumour doubling time tau2v (calculated from measurements of the tumour size effected at various stages of the evolution by assuming an exponential growth), is univocally related to 1 by a hyperbolic law so that the faster the tumour is growing themore heat generates; c) q is significanty higher and tau2v shorter in all cases where the histological examination has revealed signs of lymphatic dissemination (carcinomatous lymphangitis, lymph node metastases,...).