(1) By cross-neutralization, all the viruses were shown to be distinct; only BRD and WEX showed slight cross-reaction.
(2) 4, 331-336, 1986) and Wexford (WEX) viruses, two serotypes in the Kemerovo serogroup of orbiviruses.
(3) Comparison of the polypeptides precipitated by homologous AF revealed close similarities between BRD and WEX viruses but obvious differences between these two viruses and KEM and CNU viruses.
(4) Jones, and D. Carey, 1989, Virology 172, 428-434), whereas segment 5 of Wexford (WEX) virus and segment 6 of GI virus are the major determinants of serotype specificity (S.R.
(5) The observed dependence of Wex on the Ca2+ concentration could be interpreted quantitatively on the basis of a two-cluster model.
(6) This bacteriocin and one indicator strain (Wex) were chosen for further study.
(7) The ESR spectra were analyzed in terms of the spin exchange frequency, Wex.
(8) Kemerovo (KEM subgroup), Broadhaven (BRD) and Wexford (WEX) [Great Island (GI) sub-group], Chenuda (CNU sub-group), and Wad Medani (WM sub-group) viruses cross-reacted in immunofluorescence tests.
(9) Reassortant viruses were isolated following dual infections of cell cultures with a spontaneous temperature-sensitive (ts) mutant of WEX virus, and either NUG wild-type virus or a ts mutant of GI virus.
(10) In Vero cells infected with either KEM, BRD, WEX, or CNU viruses, 10 major (p1 to p10) and a variable number of minor virus-induced 35S-labelled polypeptides were detected.
(11) In studies with reassortants isolated following dual infection of cell cultures with WEX and GI viruses, the gene combination W4G6 (i.e., viruses deriving segment 4 from WEX virus and segment 6 from GI virus) resulted in nonpathogenic reassortants.
(12) In cross-reactions, p6 of KEM and CNU viruses, and p7 of BRD and WEX viruses were immunoprecipitated.
(13) By measuring Wex as a function of the molar percentage of labelled lecithin a distinction between a random and a heterogeneous lipid distribution could be made.
(14) The results indicate that segment 6 of GI virus is able to modulate the phenotypic expression of segment 4 of WEX virus, but not vice versa.
(15) Reassortant viruses derived from a cross between two KEM-related viruses, Great Island (GI) and Wexford (WEX), that had the heterotypic gene combination W4G6 (segment 4 of WEX virus and segment 6 from GI virus) were nonpathogenic in mice.
(16) Complement fixation tests (CFT) revealed that KEM virus was more closely related to BRD and WEX viruses than to either CNU or WM viruses.