(1) Purification of the new conjugate was performed in three sequential steps: (1) by HPLC gel filtration on TSK G3000SW to remove the unconjugated ricin: (2) by affinity chromatography on Affi-Gel Blue to separate the free antibody from the conjugate and (3) by affinity chromatography on Sepharose 6B to separate the galactose-binding IT from the non-binding moiety.
(2) Calcium-dependent binding to phenothiazine Affi-Gel confirmed that calcium binding induces conformation changes characteristic of calmodulin.
(3) In order to increase understanding of the control of inositol-1,3,4-trisphosphate kinase activity, the enzyme was highly purified from rat liver by precipitation with polyethylene glycol, MonoQ ion-exchange chromatography, heparin-agarose affinity chromatography, and a novel affinity chromatography procedure that utilized Affi-Gel resin to which InsP6 was coupled (Marecek, J.F., and Prestwich, G.D. (1991) Tetrahedron Lett.
(4) An affinity-purified fraction (APF) was obtained by passing crude somatic antigens of Trichinella spiralis muscle larvae through an Affi-Gel 10 column coupled with anti-Trichuris suis IgG.
(5) Rabbit liver tRNA nucleotidyldransferase bound to columns of Affi-Gel Blue and could be specifically eluted with tRNA.
(6) A thiol peptidase that catalyzes at near neutral pH the hydrolysis of insulin, the isolated A and B chains of insulin, and glucagon was purified from rat liver cytosol by fractionation on Sephadex G-200, Affi-Gel Blue, and Spherogel TSK-G 3000 SW.
(7) Purification involved sonic disruption of bacterial cells, ammonium sulfate fractionation, chromatography on Affi-Gel Blue, chromatofocusing, and gel filtration on Sephadex G-100.
(8) The versatility of the Affi-Gel-10 monomer affinity matrix was further demonstrated by purifying 13 mammalian arylsulfatase A enzymes to homogeneity, as assessed by Sephacryl chromatography, native and SDS gel electrophoresis.
(9) Pooled serum from normal cattle and sheep and serum from sheep, infected with two different viruses, were prepared for HPIEC by chromatography on CM-Affi-Gel Blue.
(10) A chalcone derived from a potent retinobenzoic acid (Ch55) was chosen as the ligand and fixed to an immobilized matrix by coupling with the N-hydroxysuccinimide ester of agarose (Affi-Gel 10, Bio-Rad Laboratories).
(11) The purpose of this study was to characterize a suppressive active glycopeptide (SAP) using affinity chromatography (AFFI) and explore its similarity to fibronectin (FN) degradation products.
(12) The active fraction, which eluted from an Affi-Gel Blue column between 0.10 and 0.15 M NaCl, was further resolved on a QMA anion exchange column.
(13) Angiotensin II antiserum purified by affinity chromatography was covalently coupled to Affi-gel 10 (Affi-gel 10-AB).
(14) However, both the GDP- and GTP-bound forms of the N-terminal fragment failed to bind to membranes and phosphatidylserine-linked Affi-Gel.
(15) Purification steps included several chromatography using Q-Sepharose Fast Flow, cellulose phosphate, Toyopearl HW 55 and Affi-Gel Blue.
(16) Dialyzed serum was chromatographed successively on Affi-gel blue, hydroxyapatite, DE-cellulose, phenyl-Sepharose, an affinity matrix for angiotensin converting enzyme and concanavalin-Sepharose.
(17) Plasminogen activating activity appeared resulting from the separation of UKI from PPA-UKI complex through UK-Sepharose or UK-Affi Gel 10 affinity chromatography.
(18) A 130-kDa integral membrane glycoprotein from brush border membrane was shown to bind specifically to 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonate immobilized on Affi-Gel 102 resin.
(19) One of them was araUTP-Sepharose 4B which was coupled directly with the ligand and the other was araUTP-Affi-Gel 10 which was coupled with the ligand through a spacer.
(20) The product was purified by Affi-Gel Blue followed by Bio-Rex 70 column chromatography.
Affixed
Definition:
(imp. & p. p.) of Affix
Example Sentences:
(1) This is best accomplished with a continuous stream of normal saline from a 1-I bag which is attached to an intravenous line with a 16-gauge Teflon catheter placement sleeve affixed to the distal end of the line.
(2) A device for computed tomography (CT)-guided percutaneous punctures that is not affixed to the patient and can be used even when the gantry is tilted was developed and tested.
(3) We propose a linguistic analysis of the correlation in terms of Kiparsky's (1982, 1985) level-ordering model plus an additional ordering condition on affixes: default (regular) affixes cannot serve as input to compounding processes.
(4) The correlation was similar if the meter was affixed instead directly to the scalp with collodion gel, and the clinical reliability improved.
(5) A locking mechanism with a suture affixed to the distal portion of the mushroom tip is described.
(6) The group antigen did not affix to mouse erythrocytes in vivo.
(7) By contrast, monolayer-coated dimyristoyl-PC magnetic structures are inferior with respect to both their reactivation potency and their ability to strongly affix cytochrome-c oxidase and to improve the thermal stability of the enzyme.
(8) The Antelope on Mitcham Road has a choice of three open fires where you can affix your undergarments.
(9) Photos of the first president hang from branches, have been affixed to tea stalls, and even encircle a giant banner showing Turkey's prime minister, Recep Tayyip Erdogan, dressed as Hitler.
(10) Radiopaque markers of 4 mm diameter were affixed to the pharyngeal wall by suction.
(11) Based on longitudinal data from impaired and unimpaired monolingual German-speaking children, we find a striking, statistically significant correlation: plural affixes that are used in overregularizations, namely -n or -s, are left out within compounds.
(12) An external localizing device, consisting of a series of tubes visible on MR, is affixed to an individually fitted thermoplastic mask.
(13) In this study the transverse flexural stiffnesses of five preformed arch wires were quantified in each of three activation directions at five separate sites on simulated dental arches to which appliances were affixed.
(14) In previous studies it was shown that fixed platelets bearing covalently-bound fibrinogen participate passively in release-related aggregation, and that thrombospondin is the released compound which specifically and selectively recognizes the affixed fibrinogen.
(15) The revisions were performed not for aseptic loosening but for causes in which all the implants were securely affixed to bone.
(16) The in vitro experiment involved palladium 103 seeds placed into a Silastic seed holder, which was affixed into standard 14-mm gold eye plaques.
(17) Availability of distinctive cues affixed on the objects' sides facilitated solution of the symmetrical orientations.
(18) RNA polymerase bound last to form a preinitiation complex, but it was less stably affixed than any of the factors.
(19) While affixing your e-signature to an online petition is a new and somewhat direct way to "petition your government for a redress of grievances", I am most concerned with advocating for more immediate and effective manners of protest.
(20) Facebook Twitter Pinterest The end of the paper tax disc Facebook Twitter Pinterest After more than 90 years affixed to British motorists’ cars, the tax disc is no longer needed from today.