(1) We used the polymerase chain reaction (PCR) to amplify the breakpoint area of alpha-thalassemia-1 of Southeast Asia type and several parts of the alpha-globin gene cluster to make a differential diagnosis between alpha-thalassemia-1 and Hb Bart's hydrops fetalis.
(2) We have investigated the increase in the spcDNA population upon cycloheximide treatment of individual sequences, which are found to amplify differentially.
(3) We conclude that only one of the alleles was amplified and that either allele could be amplified with respect to both the SphI and PvuII polymorphisms.
(4) RNA preparations were reverse-transcribed, amplified by a polymerase chain reaction (PCR), and separated by electrophoresis.
(5) It was previously demonstrated that multidrug-resistant Chinese hamster cell lines contain an amplified, transcriptionally active DNA sequence designated mdr.
(6) Chloroquine increased the binding of 125I-labeled insulin to rat hepatoma cells (R-Y121B) at 4 degrees C. The effect of chloroquine on insulin binding was amplified at 23 degrees C, and a large increase in cell-associated radioactivity was observed.
(7) Overexpression of asparagine synthetase in beta-aspartyl hydroxamate-resistant lines without amplified copies of the gene was also correlated with DNA hypomethylation.
(8) (iii) The insertion plasmid pRSD102(R388::Tn1721) has conserved the capacity of the original plasmid pRSD1 to amplify the 3.55 Mdal-tet region.
(9) To identify the molecular defect in a patient with the intermediate form of the disease, arylsulfatase B mRNA from his fibroblasts was reverse-transcribed, amplified by the polymerase chain reaction, and subcloned.
(10) In addition to the fatigue tester and the pulse duplicator, a signal conditioner, a DC amplifier, an analog-to-digital converter, and a digital microcomputer comprised the essential hardware.
(11) The sensitivity of a PCR system to amplify the long terminal repeat (LTR) sequence of HIV-1 was not affected by the irradiation procedure; however, the ultimate sensitivity of a PCR system for the amplification of an early gene promotor sequence of the CMV genome was reduced 1000-fold.
(12) Thus MTX combined with PALA selected cells with an 'amplificator' phenotype (an increased ability to amplify parts of the genome).
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) As an extension to the variety of existing techniques using polymorphic DNA markers, the Random Amplified Polymorphic DNA (RAPD) technique may be used in molecular ecology to determine taxonomic identity, assess kinship relationships, analyse mixed genome samples, and create specific probes.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) Whether we would use that to support and amplify the community ideals already present or go the way of gentrification remained to be seen.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) The action of exogenous etiologic agents is usually mediated in part or amplified by endogenous mediators that very often exert biphasic, i.e., damaging and protective, effects.
(19) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
(20) The PCR amplified a 375-bp DNA fragment which was cloned and sequenced; the deduced amino acid sequence had significant identity with known TS sequences, including strict conservation of all phylogenetically invariant TS amino acid residues.
Gain
Definition:
(n.) A square or beveled notch cut out of a girder, binding joist, or other timber which supports a floor beam, so as to receive the end of the floor beam.
(v. t.) That which is gained, obtained, or acquired, as increase, profit, advantage, or benefit; -- opposed to loss.
(v. t.) The obtaining or amassing of profit or valuable possessions; acquisition; accumulation.
(n.) To get, as profit or advantage; to obtain or acquire by effort or labor; as, to gain a good living.
(n.) To come off winner or victor in; to be successful in; to obtain by competition; as, to gain a battle; to gain a case at law; to gain a prize.
(n.) To draw into any interest or party; to win to one's side; to conciliate.
(n.) To reach; to attain to; to arrive at; as, to gain the top of a mountain; to gain a good harbor.
(n.) To get, incur, or receive, as loss, harm, or damage.
(v. i.) To have or receive advantage or profit; to acquire gain; to grow rich; to advance in interest, health, or happiness; to make progress; as, the sick man gains daily.
Example Sentences:
(1) Anti-corruption campaigners have already trooped past the €18.9m mansion on Rue de La Baume, bought in 2007 in the name of two Bongo children, then 13 and 16, and other relatives, in what some call Paris's "ill-gotten gains" walking tour.
(2) The metabolism of [1,3-14C]benzo[f]quinoline (BfQ) by liver microsomes from control, 3-methylcholanthrene (3-MC)-pretreated and phenobarbital (PB)-pretreated rats has been investigated in order to gain insights into the effect of mixed function oxidase inducers on the types and levels of specific metabolites as formed in vitro.
(3) Helsby, who joined the estate agent in 1980, saw his basic salary unchanged at £225,000, but gains a £610,000 windfall in shares, available from May, as well as a £363,000 increase in cash and shares under the company profits-sharing scheme.
(4) Breast conserving surgery in patients with small tumors combined with radiation therapy has gained wide popularity due to better cosmetic results without significant changes in survival.
(5) Abruptly changing cows from one feeding system to another did not influence milk yield, milk composition, or body weight gain.
(6) Physicians working in the emergency room gained 14.7% during that time of day the PNP was present.
(7) The reference cohort consisted of 1725845 men otherwise gainfully employed.
(8) "We presently are involved in a number of intellectual property lawsuits, and as we face increasing competition and gain an increasingly high profile, we expect the number of patent and other intellectual property claims against us to grow," the company said.
(9) I have heard from other workers that the list has also been provided to the law enforcement authorities,” Gain says.
(10) He also plans to build a processing facility where tourists can gain firsthand experience of the fisheries industry, and to open a restaurant.
(11) At first it looked as though the winger might have shown too much of the ball to the defence, yet he managed to gain a crucial last touch to nudge it past Phil Jones and into the path of Jerome, who slipped Chris Smalling’s attempt at a covering tackle and held off Michael Carrick’s challenge to place a shot past an exposed De Gea.
(12) These results suggest that aluminum is able to gain access to the central nervous system under normal physiological conditions.
(13) Averaged across all dietary levels, tiamulin resulted in a 14.1% improvement in gain and a 5.7% improvement in feed:gain ratio during the first 28 to 35 d of the experiment (to 30 kg).
(14) In the first trial to investigate the effect of tick control, significant improvements in liveweight gain (LWG) occurred only in periods of medium to high challenge with adult Amblyomma variegatum.
(15) These results suggest that a lowered basal energy expenditure and a reduced glucose-induced thermogenesis contribute to the positive energy balance which results in relapse of body weight gain after cessation of a hypocaloric diet.
(16) A variety of homobifunctional crosslinking agents have been used to gain insight into the nature of the murine interleukin 3 (mIL-3) receptor.
(17) As a result, each may eventually gain widespread use after further development.
(18) Gains in gait pattern, ease of bracing, and reduced pelvic obliquity were noted.
(19) At 24 days of age, the pups of HP, M and M-F diet groups, only gained 48%, 30% and 18% respectively, in their body weight, whereas the body-length parameters (LNC and LNRC) showed a reduction of 20%, 35%, and 45%, respectively for the same diet groups.
(20) Among the agents triggering such an infection Chlamydia (30.9% of the cases of non-gonorrhoic urethritis), as well as mycoplasma, ureaplasma, anaerobic bacteria and herpes simplex viruses have gained particular significance.