(1) A derivative spectroscopic method and a HPLC-method are described for the determination of the annatto dye-stuffs, norbixin and bixin, in cheese.
(2) These techniques will detect the presence of coal tar dyes, imidazole, histamine, histidine, cochineal, beet powder, annatto, alkanet, carotene, paprika, saffron, turmeric, and materials causing discoloration in meats.
(3) Similar morphology occurs in oncocytic tumours; riboflavin-deficient rats and mice; rats given non-lethal doses of hypoglycin; dogs treated with annatto extracts; and hibernating or starving frogs.
(4) Annatto dye is an orange-yellow food coloring extracted from the seeds of the tree Bixa orellana.
(5) Annatto extract, a commonly used food colour in edible fats e.g.
(6) Annatto dye is a potential rare cause of anaphylaxis.
(7) We present a patient who developed urticaria, angioedema, and severe hypotension within 20 minutes following ingestion of milk and Fiber One cereal, which contained annatto dye.
(8) The patient had a strong positive skin test to annatto dye, while controls had no response.
(9) Previously reported adverse reactions associated with annatto dye have included urticaria and angioedema.
(10) Therefore, this technique may be used within the rigorous Austrian regulations or for controlling the quantities and limits of annatto dye-stuffs in cheese, if its application is allowed.
(11) The red powdery extract from the seeds of the annatto, Bixa orellana, is a well known food colouring.
(12) The nondialyzable fraction of annatto dye on SDS-PAGE demonstrated two protein staining bands in the range of 50 kD.
(13) Annatto dye may contain contaminating or residual seed proteins to which our patient developed IgE hypersensitivity.