(n.) Rubbish or refuse consisting of broken rock containing little or no ore.
Example Sentences:
(1) Similar analyses demonstrate that efficient excision can occur with two other different sets of wild-type arm-type sites in attL and attR.
(2) The results indicate that pSG1int is flanked at attL by a functional tRNA(ser) gene and at attR by a 60-nt sequence of the 3' end of the same tRNA(ser) gene.
(3) A 1200-bp region of P2 DNA containing the int gene and attP, the prophage hybrid ends attL and attR, and one bacterial attachment site, the preferred site locI from E. coli strain C, have all been sequenced.
(4) Moreover, lambda DNA containing a pBR322 derivative flanked by the lambda attL and attR sites could be specifically recircularized by excisive lambda recombination to yield the pBR322 derivative.
(5) Excision (intraplasmid att site recombination) was examined by constructing plasmids carrying attL and attR or two attP sites separating CmR from KmR and by following segregation of the markers in various hosts.
(6) To map the protein-protein and protein-DNA interactions involved in lambda site-specific recombination, Int cleavage assays with suicide substrates, nuclease protection patterns, gel retardation experiments, and quantitative Western blotting were applied to wild-type attL and attL mutants.
(7) The pSG1 plasmid integration site (attP) and the pSG1int-chromosome boundaries (attL and attR) were cloned and sequenced.
(8) Similar plasmids containing the two junction segments (attL and attR regions) between the phage genome and the lysogenic host chromosome were also prepared.
(9) The results lead to a model in which one IHF molecule bends the attL DNA and forms a higher order complex with the three bivalent Int molecules required for excisive recombination.
(10) The attL of the second bacterial attachment site present in the host SmaI fragment 7 and the leftmost part of phage S2 type B DNA of its genome organization (Piekarowicz et.
(11) Nucleotide sequencing of attP, attB, attL, and attR revealed a 57-base-pair sequence common to all sites with no duplications of adjacent plasmid or chromosomal sequences in the integrated state, indicating that integration takes place through conservative, reciprocal strand exchange.
(12) Using site-directed mutagenesis, we have constructed a related set of point mutations within each of the five Int "arm-type" binding sites located within attP, attL and attR.
(13) The attachment site of pIJ408 (attP) and the junctions of its integrated form with the chromosomal DNA in S. glaucescens (attL and attR) contain an identical 43 bp sequence.
(14) This recombination was directional, since no reaction was observed between plasmids containing attR and attL sites.
(15) Oligonucleotides derived from the sequence of the attP-containing fragment enabled us to amplify predicted junction fragment sequences and thus to identify attL, attR, and attB.
(16) With these novel substrates we show that Xis specifically promotes the first strand exchange and that attL enhances Int cleavage at the top-strand site of attR.
(17) A 58-bp sequence (att) present in both pSAM2 (attP) and S. ambofaciens strain DSM40697 (attB) attachment regions is found at the boundaries (attL and attR) of integrated pSAM2 in S. ambofaciens strain ATCC23877.
(18) The endpoints of the bacterial DNA segments in lambdagal3 and lambdagal8 are physically mapped in relation to attL.
(19) By combining the attL and attR sequences, the attB sequences of locations II, III, and H have been deduced.
(20) The DNA nucleotide sequences of attP, attB attR and attL were determined.
Wattle
Definition:
(n.) A twig or flexible rod; hence, a hurdle made of such rods.
(n.) A rod laid on a roof to support the thatch.
(n.) A naked fleshy, and usually wrinkled and highly colored, process of the skin hanging from the chin or throat of a bird or reptile.
(n.) Barbel of a fish.
(n.) The astringent bark of several Australian trees of the genus Acacia, used in tanning; -- called also wattle bark.
(n.) The trees from which the bark is obtained. See Savanna wattle, under Savanna.
(v. t.) To bind with twigs.
(v. t.) To twist or interweave, one with another, as twigs; to form a network with; to plat; as, to wattle branches.
(v. t.) To form, by interweaving or platting twigs.
Example Sentences:
(1) The group given the small multilamellar positively charged liposome also showed significant delayed-type hypersensitivity (wattle swelling) (P less than or equal to 0.05).
(2) Beta stimulation with isoproterenol markedly reduced R and increased Q in normothermic birds, suggesting the presence of beta receptors in the wattle vasculature.
(3) The presence of cytoplasmic dihydrotestosterone receptors in the lungs, the comb, the wattle, and the ear lobes of the cock was demonstrated by sucrose density-gradient centrifugation.
(4) The mud and wattle huts in which pupils were taught have now been replaced with seven permanent classrooms.
(5) Photograph: Eamonn Mccabe for the Guardian When she was a child living in a Tudor cottage in rural Cheshire, the walls were lumpy, and badly painted, wattle and daub.
(6) The faecal output of strongyle eggs was significantly related to breed, polledness, presence of wattles and age.
(7) Wattle reactions to an Eimeria tenella antigen and phytohemagglutinin (PHA) were studied in chickens infected with E. tenella.
(8) Until now the school has used temporary mud-and-wattle structures with grass-thatched roofs that sway in the wind or, in rough weather, simply collapse.
(9) Instead, let Australia summon up the sentiments of Henry Lawson's iconic 1891 poem, Freedom on the Wallaby , for today it is not the rebel's blood but a callous disregard for the vulnerable that "stains the wattle".
(10) The preoperative diagnosis may be suggested by the "turkey wattle sign" (i.e., fluctuation in the size of the mass with bending the head downward).
(11) Nitrogen and atom-% 15N excess (15N') were determined in the bones, the feathers and the remaining body (skin, lungs and windpipe, head with comb and wattle, lower leg without bones and with skin, pancreas and fatty tissue).
(12) Alpha blockade with phenoxybenzamine also resulted in pronounced vasodilatation, suggesting tonic alpha-sympathetic tone in the wattle vasculature under normothermic conditions.
(13) During moderate cooling, vasoconstriction in the feet and wattles of broody hens (but not of non-broody hens) freed non-nutrient blood flow for redistribution to the brood patches.
(14) Although delayed hypersensitivity was confirmed by delayed wattle reaction in 2-month-old chickens sensitized with living S pullorum, the sensitization did not markedly affect phagocytic and bactericidal activities.
(15) 5-HT and NE each depressed significantly the wattle response in 3 and 6 week old chicks.
(16) At 6 weeks of age, chickens were injected with 100 micrograms purified PHA-P. Wattle thickness measurements were taken 4, 24, 48, 72 and 96 h after injection.
(17) Injections were given 12 h prior to, at the time of, and at 12 and 24 h after an intradermal wattle injection with PHA-P.
(18) A rare case of a symptomatic venous anomaly of the parotid gland is described in a 14-year-old female patient who presented with Turkey Wattle sign.
(19) The study was undertaken in spring (n = 263 goats) and autumn (n = 165); the breed, age, polledness, absence or presence of wattles, and reproductive status were recorded for each goat.
(20) A double-wattled cassowary died following a clinical course of severe diarrhea, anorexia, and polydypsia.