(1) First, the C6-NBD moiety was replaced by a bare [14C]octanoyl chain or by the even more bulky fluorophores 8-bimanoylthio-octanoyl (C8-bimane) and 8-diethylaminocoumarin-octanoyl (C8-DECA).
(2) Monobromobimane (mBBr, bimane), a compound that penetrates cells and forms a fluorescent adduct with thiol groups, was used to asses the significance of thiols in platelet function.
(3) Bimane inhibited epinephrine-induced arachidonate release in gel-filtered platelets, possibly because it abolished the primary aggregation necessary for this release.
(4) The fluorescent GSH-bimane adduct, once formed within the cell, was not released from the cell.
(5) The bimane-modified lysine-13 protein, and to a lesser extent that modified at lysine 8, show the interesting effect of enhanced complex formation with cytochrome c oxidase when subjected to pressure, possibly because of entrapment of water at the newly created interface of the complex.
(6) Roughly a linear increase in emitted fluorescence, thus detection sensitivity, is observed with an increasing number of bimane markers.
(7) The bimane derivatives were identified and quantified by HPLC and fluorescence.
(8) Among these bimane substrates, substrate 3 was shown to be a potent fluorogenic substrate for microdetermination of the ACE level in human serum.
(9) The fluorescence of 9,10-dioxa-syn-3,4,6,7-tetramethylbimane (bimane) was found to be quenched in the presence of guanosine 5'-monophosphate.
(10) Bovine brain S100 alpha alpha, S100a (alpha beta), and S100b (beta beta) protein dimers were labeled with the sulfydryl-specific fluorescent probes monobromo(trimethylammonio)bimane (bimane) and 6-acryloyl-2-(dimethylamino)naphthalene (acrylodan) at cysteines-85 alpha and -84 beta.
(11) Intact platelets were reacted with 1-10 mM monobromobimane or monobromo(trimethylammonio)bimane or 50-100 microM N-dansylaziridine for 30-60 min at 37 degree C. The platelets were then washed, solubilized in 1% Triton X-100, and analyzed by nonreduced-reduced polyacrylamide gel electrophoresis either directly or indirectly after immunopurification of GPIb.
(12) An assay using a bimane derivative has been developed to detect free glutathione (GSH) in individual viable cells by flow cytometry.
(13) Spectroscopic studies on the fluorescence properties of labeled S100b with the fluorescent probes bimane and acrylodan at Cys-84 beta confirmed the antagonistic effect of Ca2+ and Zn2+ with respect to the conformational properties of the protein.
(14) Established ADP-induced aggregation was reversed by bimane, and fibrinogen binding to ADP-stimulated platelets was inhibited, an effect mainly due to decreased number of binding sites.
(15) Phospholipids labeled with (dialkylamino)coumarin moieties, and to a slightly lesser extent those labeled with a bimane group, exhibit a strong and stable blue fluorescence in phospholipid dispersions that is relatively insensitive to the physical state of the lipid phase.
(16) These probes circumvent the use of an ultraviolet (UV)-excitation system as required by bimane-based dyes and allow combination with probes for other cellular parameters.
(17) The essential steps in the assay include (i) conversion of homocysteine disulfides to free homocysteine with borohydride reduction; (ii) conjugation of homocysteine with monobromobimane; (iii) separation of homocysteine-bimane from other plasma thiol-bimane adducts by reverse-phase high-performance liquid chromatography; and (iv) detection and quantitation of homocysteine-bimane by fluorometry.
(18) We have now labeled the single reactive cysteine covalently with monobromobimane and isolated and sequenced the bimane-containing cyanogen bromide peptide and identified the cysteine as Cys-206.
(19) Quantitation of the bimane adducts of cysteine and glutathione is achieved by reversed phase high performance liquid chromatography, whereas quantitation of the corresponding adducts of protein thiols is achieved by fluorescence spectroscopy following protein precipitation.
(20) Biman ual palpation may allow detection of the ectopic IUD.
Hand
Definition:
(n.) That part of the fore limb below the forearm or wrist in man and monkeys, and the corresponding part in many other animals; manus; paw. See Manus.
(n.) That which resembles, or to some extent performs the office of, a human hand
(n.) A limb of certain animals, as the foot of a hawk, or any one of the four extremities of a monkey.
(n.) An index or pointer on a dial; as, the hour or minute hand of a clock.
(n.) A measure equal to a hand's breadth, -- four inches; a palm. Chiefly used in measuring the height of horses.
(n.) Side; part; direction, either right or left.
(n.) Power of performance; means of execution; ability; skill; dexterity.
(n.) Actual performance; deed; act; workmanship; agency; hence, manner of performance.
(n.) An agent; a servant, or laborer; a workman, trained or competent for special service or duty; a performer more or less skillful; as, a deck hand; a farm hand; an old hand at speaking.
(n.) Handwriting; style of penmanship; as, a good, bad or running hand. Hence, a signature.
(n.) Personal possession; ownership; hence, control; direction; management; -- usually in the plural.
(n.) Agency in transmission from one person to another; as, to buy at first hand, that is, from the producer, or when new; at second hand, that is, when no longer in the producer's hand, or when not new.
(n.) Rate; price.
(n.) That which is, or may be, held in a hand at once
(n.) The quota of cards received from the dealer.
(n.) A bundle of tobacco leaves tied together.
(n.) The small part of a gunstock near the lock, which is grasped by the hand in taking aim.
(v. t.) To give, pass, or transmit with the hand; as, he handed them the letter.
(v. t.) To lead, guide, or assist with the hand; to conduct; as, to hand a lady into a carriage.
(v. t.) To manage; as, I hand my oar.
(v. t.) To seize; to lay hands on.
(v. t.) To pledge by the hand; to handfast.
(v. t.) To furl; -- said of a sail.
(v. i.) To cooperate.
Example Sentences:
(1) HSV I infection of the hand classically occurs in children with herpetic stomatitis and in health care workers infected during patient care delivery.
(2) On the other hand, the LAP level, identical in preterms and SDB, is lower than in full-term infants but higher than in adults.
(3) On the other hand, human IL-9, which is a homologue to murine P40, was cloned from a cDNA library prepared with mRNA isolated from PHA-induced T-cell line (C5MJ2).
(4) We sought additional evidence for an inverse relationship between functional CTL-target cell affinity on the one hand, and susceptibility of the CTL-mediated killing to inhibition by alpha LFA-1 and alpha Lyt-2,3 monoclonal antibodies on the other hand.
(5) Handing Greater Manchester’s £6bn health and social care budget over to the city’s combined authority is the most exciting experiment in local government and the health service in decades – but the risks are huge.
(6) Then a handful of organisers took a major bet on the power of people – calling for the largest climate change mobilisation in history to kick-start political momentum.
(7) On the other hand, after exposure to BrdUrd, neuron specific enolase decreased in NB-1 and SK-N-DZ and increased in GOTO.
(8) Theresa May signals support for UK-EU membership deal Read more Faull’s fix, largely accepted by Britain, also ties the hands of national governments.
(9) The NORPLANT-2 rod system on the other hand consists of only 2 rods.
(10) Symptomatic improvement was obtained in 14 of the 15 hands, and sensory-evoked response improved in 13 hands.
(11) Michael James, 52, from Tower Hamlets Three days after telling his landlord that the flat upstairs was a deathtrap, Michael James was handed an eviction notice.
(12) Patrice Evra Evra Handed a five-match international ban for his part in the France squad’s mutiny against Raymond Domenech at the 2010 World Cup, it took Evra almost a year to force his way back in.
(13) DI James Faulkner of Great Manchester police said: “The men and women working in the factory have told us that they were subjected to physical and verbal assaults at the hands of their employers and forced to work more than 80-hours before ending up with around £25 for their week’s work.
(14) On the other hand, the majority of gynecologic patients with pelvic infections are young and healthy.
(15) The pons, on the other hand, has a bioelectrical activity of its own during PS, i.e., the ponto-geniculo-occipital spikes (PGO).
(16) One-nation prime ministers like Cameron found the libertarians useful for voting against taxation; inconvenient when they got too loud about heavy-handed government.
(17) On the other hand, the limbic after-discharges to the hippocampal or amygdaloid stimulation were enhanced by Z. mioga as well as chlorpromazin, but they were inhibited by diazepam.
(18) On the other hand, as a cross-reference experiment, we developed a paper work test to do in the same way as on the VDT.
(19) It is entirely proper for serving judges to set out the arguments in high-profile cases to help public understanding of the legal issues, as long as it is done in an even-handed way.
(20) In a separate exclusive interview , Alexis Tsipras, the increasingly powerful 37-year-old Greek politician now regarded by many as holding the future of the euro in his hands, told the Guardian that he was determined "to stop the experiment" with austerity policies imposed by Germany.