(1) We have examined the recombination potential of BLIN-1 by characterizing several independently derived kappa-expressing subclones for DNA rearrangement and V kappa gene usage.
(2) The BLIN-1 cell line is, to our knowledge, the first defined human model for examining this critical developmental stage in human B cell ontogeny.
(3) By polymerase chain reaction analyses, we detected transcripts from rearranged V-J-C kappa genes as well as transcripts from germline J-C kappa and V kappa in BLIN-1 cells induced to rearrange the kappa locus.
(4) Our collective results indicate that: (a) BLIN-1 can be induced to rearrange the kappa locus, and this correlates with the expression of germline kappa locus transcripts that may play a role in activating or targeting gene rearrangement; and (b) active rearrangement and usage of V genes representing different kappa families suggest that, like in the mouse, repertoire diversification in humans occurs in the presence of a fixed heavy chain rearrangement.
(5) Binding studies using radiolabeled IL-3 revealed the presence of approximately 200 high affinity IL-3 receptors on the BLIN-1 cell line, with a KD of 150pM.
(6) Analysis of Ig gene rearrangement and Ig expression in a series of BLIN-1 subclones show that the cells spontaneously rearrange kappa light chain genes, leading to the differentiation of surface kappa-negative pre-B cells into surface kappa-positive B cells.
(7) In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate.
(8) The human pre-B acute lymphoblastic leukemia cell line, BLIN-1, has been previously shown to undergo kappa light chain rearrangement in vitro, making it a valuable resource for analyzing pre-B to B cell differentiation.
(9) This cell line, designated BLIN-1, was initially established in tissue culture medium containing low m.w.
(10) BLIN-1 cells have a 9p chromosomal abnormality, identical to the abnormality present in the leukemic blasts from the patient's original bone marrow aspirate.
(11) The immunologic phenotype of BLIN-1 is consistent with a cell arrested at the pre-B cell stage of development.
Lin
Definition:
(v. i.) To yield; to stop; to cease.
(v. t.) To cease from.
(n.) A pool or collection of water, particularly one above or below a fall of water.
(n.) A waterfall, or cataract; as, a roaring lin.
(n.) A steep ravine.
Example Sentences:
(1) Lin Homer's CV Lin Homer left local for national government in 2005, giving up a £170,000 post as chief executive of Birmingham city council after just three years in post, to head the Immigration Service.
(2) Previous FTIR measurements have identified several tyrosine residues that change their absorption characteristics between light-adapted BR and dark-adapted BR, or between intermediates K and M [Dollinger, G., Eisenstein, L., Lin, S.-L., Nakanishi, K., Odashima, K., & Termini, J.
(3) If the eye shielding block cannot be placed at the optimal shielding point, a simple coin placed on the eye lid surface will also reduce the lens dose substantially when a regular eye shielding block is placed on the blocking tray (Lin's coin effect).
(4) I didn't realise it would cover the country for the next 10 years," Lin says.
(5) Several N-nitrosamines (NDMA, NDEA, NMBzA, NPyr, NPip, and NSAR) in gastric juice collected from Lin-Xian inhabitants have been detected.
(6) lin-17 mutant animals also show defects in the position of the PVM cell and the PLM axons.
(7) The agency’s current chief executive, Lin Homer, is due to face the Commons public accounts committee, chaired by the Labour MP Margaret Hodge.
(8) Lin Hatfield Dodds from Uniting Care said the heavy lifting in the budget was being done by families.
(9) Perry Link of Princeton University compared the case to the mysterious 1971 death of the senior communist leader Lin Biao in a plane crash.
(10) One of them has the properties of a plasma membrane Ca2+-pump (Lin, S.-H. (1985) J. Biol.
(11) By correlating the fates of Z1.ppp and Z4.aaa with the lin-12 genotype of nearly every cell in these mosaics, we conclude that lin-12 function is VU cell autonomous.
(12) Native human Glu-plasminogen (Glu1-Asn791) was previously shown to have a radius of gyration of 39 A and a shape best described by a prolate ellipsoid [Mangel, W. F., Lin, B., & Ramakrishnan, V. (1990) Science 248, 69-73].
(13) CB-a encompasses the COOH-terminal segment of residues 659-756, according to the sequence of adult chicken gizzard caldesmon (Bryan, J., Imai, M., Lee, R., Moore, P., Cook, R.G., and Lin, W.G.
(14) The absolute means of the differences between models from disinfected or nondisinfected impressions reached from 0.05%lin to 0.19%lin.
(15) When a 7.9-magnitude earthquake ripped through Sichuan province in May 2008, Lin Tianhong, a 29-year-old reporter at China Youth Daily , was one of the first to volunteer to head into the disaster zone.
(16) In the present study, employing over 100 DNA samples obtained from Lin-xian patients who underwent surgery for esophageal cancer, we have found a significant frequency of amplification of either the human epidermal growth factor receptor (HER-I) gene or the c-myc oncogene.
(17) Keith Vaz , the chairman of the Commons home affairs select committee, which accused the former UKBA head, Lin Homer, of "catastrophic leadership failure" while she was in charge, congratulated May for "delivering the lethal injection" to the organisation.
(18) A modification of Lin's systematic DNA sequencing strategy is described.
(19) Lin Homer has been announced as the next chief executive of HM Revenue & Customs (HMRC).
(20) We have previously defined a subset of High Proliferative Potential Colony Forming Cells (HPP-CFC), derived from murine marrow purified for early progenitors expressing the Stem Cell Antigen (SCA+) and lacking terminal lineage markers (Lin-), which are responsive to multiple cytokines in combination.