(v. t.) To correct by punishment; to inflict pain upon the purpose of reclaiming; to discipline; as, to chasten a son with a rod.
(v. t.) To purify from errors or faults; to refine.
Example Sentences:
(1) The setbacks have made for a chastening climbdown for Ineos, given the confidence with which Ratcliffe announced his intentions in 2014.
(2) Ai emerged from his ordeal in June, far slimmer – having lost almost 10kg, some of which he has regained – and apparently chastened.
(3) They scramble quickly back to the feet, looking chastening but not undaunted - which is just as well because if they do not finish today's stage all their previous exertions will have been for nothing, like being expelled from college on the eve of graduation day.
(4) He is learning to live with the regrets - it is a chastening experience after a 45-year unblemished business career.
(5) But you saw the spirit in the team tonight, we kept fighting to the end.” For Leicester it was another chastening evening and one that ended with Nigel Pearson embroiled in an ugly row, in which expletives were exchanged, with a fan.
(6) Cameron appeared chastened, felt betrayed by Berlin.
(7) Liverpool’s £32.5m signing played the full 90 minutes and it must have been a chastening experience for the Belgium international.
(8) There was little evidence that message got through to Pellegrini's players on an afternoon that was as chastening for Manchester City as it was glorious for Cardiff.
(9) After all, Wilson has experienced not just the thrill of mega-success (Kaiser Chiefs' debut album Employment sold more than 2m copies) but early years of struggle and rejection, and a chastening period of diminishing returns that has seen each album sell less than its predecessor.
(10) Fanning's bristling performance, complete with clipped English accent, is the most chastening of the film's surprises.
(11) This is a chastening championship and it will be relished all the more for it.
(12) After a chastening week that included defeats to Liverpool and Juventus, they produced a characteristic rejoinder.
(13) His position was precarious before the game and it must have been a chastening walk for the manager when he turned on his heel at the final whistle and headed for the tunnel.
(14) It's a signal about a commitment to fairness within a more chastened and fractious coalition.
(15) Grant insisted he had got his information from "a very highly placed source", but seemed suitably chastened.
(16) The figure is down from 21 in the previous year, as a chastened BBC tries to live within the means imposed by a six-year licence fee freeze.
(17) Chastened, he co-founded a talent agency and clawed his way back to LA, and success, until a deal soured and ruined him by 2007.
(18) In its third byelection humiliation in nine weeks, Labour saw its majority of more than 13,000 evaporate in a swing of more than 22% to the SNP, a reversal even more chastening than the loss of Crewe and Nantwich in May on a 17% swing.
(19) James's speech helped consolidate his position as heir apparent to his father's role, but since then his capital within News Corp has declined considerably and James and Rupert have been chastened by the fallout from the hacking scandal at their News of the World tabloid – now closed – which prompted outrage last year, damaging the value and reputation of News Corp and the Murdoch family.
(20) But, although some historians – including David Starkey, Antony Beevor and Niall Ferguson – backed the move , the DfE seems to have been chastened by the reaction, with Gove indicating last month to the Commons education select committee that the curriculum would be changed for its next draft.
Purify
Definition:
(v. t.) To make pure or clear from material defilement, admixture, or imperfection; to free from extraneous or noxious matter; as, to purify liquors or metals; to purify the blood; to purify the air.
(v. t.) Hence, in figurative uses: (a) To free from guilt or moral defilement; as, to purify the heart.
(v. t.) To free from ceremonial or legal defilement.
(v. t.) To free from improprieties or barbarisms; as, to purify a language.
(v. i.) To grow or become pure or clear.
Example Sentences:
(1) Competition with the labelled 10B12 MAb for binding to the purified antigen was demonstrated in sera of tumor-bearing and immune rats.
(2) Previous attempts to purify this enzyme from the liquid endosperm of kernels of Zea mays (sweet corn) were not entirely successful owing to the lability of partially purified preparations during column chromatography.
(3) The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate.
(4) The quaternary structure of ribonucleotide reductase of Escherichia coli was investigated, with the use of purified B1 and B2 proteins and bifunctional cross-linking agents.
(5) Western blot analysis of these mitochondria using an antibody against carnitine palmitoyltransferase II purified from beef heart demonstrates a 68-kDa protein, which under ischemic conditions apparently is decreased by 2 kDa.
(6) ASF-II was purified to apparent homogeneity by using concanavalin A-agarose affinity chromatography, DEAE-cellulose chromatography, alumina gel adsorption, and isoelectric focussing techniques.
(7) The enzyme was solubilized by Triton X-100 and purified approximately 480-fold by gel filtration and affinity chromatography on alanine methyl ketone-AH-Sepharose 4B.
(8) The DNA untwisting enzyme has been purified approximately 300-fold from rat liver nuclei.
(9) heterografts of GW-39, a CEA-producing colonic tumor of human origin, was demonstrated in radioimmunoassay using radioiodinated CEA purified from GW-39.
(10) Four new monochain phospholipases were purified from the Oxyuranus scutellatus (taipan) venom.
(11) By growing purified human cytotrophoblasts under serum-free conditions and manipulating the culture surface, we were able to disassociate morphologic from biochemical differentiation.
(12) Lysates of cells were compared to purified DNA as PCR template.
(13) Histone mRNA, labeled with 32P or 3H-methionine during the S phase of partially synchronized HeLa cells, was isolated from the polyribosomes and purified as a "9S" component by sucrose gradient sedimentation.
(14) Proliferation of quiescent hematopoietic stem cells, purified by cell sorting and evaluated by spleen colony assay (CFU-S), was investigated by measuring the total cell number and CFU-S content and the DNA histogram at 20 and 48 hours of liquid culture.
(15) Adult nonpregnant female rhesus monkeys fed purified diets containing 100 or 4 ppm zinc for 1 yr were mated then studied through midgestation.
(16) Monoclonal antibodies (mAbs) against the soluble form (S-COMT) of catechol-O-methyltransferase (COMT, EC 2.1.1.6) were produced using a purified preparation of the enzyme from pig liver as antigen.
(17) Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a repressor-operator dissociation constant of about 0.2 nM in 0.12 M salt at 37 degrees , a value consistent with the extent of trp operon regulation in vivo.
(18) The specificity of the assay was established by competitive displacement of 125I-labeled arginine-rich protein from its antiserum by arginine-rich protein and lipoproteins containing this protein, but not by rat albumin or other purified apolipoproteins.
(19) To augment the in vitro expansion of LAK cells, we added highly purified human recombinant interleukin-2, phytohemagglutinin and accessory cells (Uc cells) to the LAK culture system, with which huge number of LAK cells (LAK-L) were generated from originally small number of peripheral blood lymphocytes of cancer patients.
(20) This enzyme was purified to homogeneity and exhibited an apparent molecular weight of 36,000 on sodium dodecyl sulfate gels and 180,000 on a TSK G-3000SW column in the presence of Triton X-100.