(1) DNA in situ is progressively denatured when the cells or nuclei are treated with increasing concentration of acridine orange (AO).
(2) We propose that during the detergent solubilization the acidic phospholipids protect the transport systems against denaturation by preventing delipidation.
(3) Denaturation studies demonstrated that less than 50% of protein content of C. psittaci was denatured after 1 h of sonication, only 5% in the case of C. trachomatis.
(4) Our results also showed a good correlation between the importance of deposits and the presence of denatured DNA-anti-denatured-DNA circulating complexes.
(5) Refolding was observed by injection of denatured protein into columns having isocratic concentrations in the transition and native base-line zones.
(6) Extraction of liposomes containing guanylate cyclase with 0.2% Lubrol PX resulted in the recovery of 85% of the original amount of added activity, suggesting that the decrease in maximal velocity was not due to enzyme denaturation.
(7) Single-stranded circles did not form if a limited number of nucleotides were removed from the 3' ends of native molecules by Escherichia coli exonuclease III digestion prior to denaturation and annealing.
(8) Denatured DNA and histones were inhibitory, but native DNA and its histone complex were not inhibitory.
(9) Both enzymes are capable of catalyzing the refolding of thermally denatured type III collagen.
(10) We investigated the denaturation of tetrameric 20 beta-hydroxysteroid dehydrogenase (20R)-17 beta,20 beta,21-trihydroxysteroid:NAD+ oxidoreductase, EC 1.1.1.53) to find out whether intermediate states are formed during the process.
(11) Thermal-denaturation studies showed that this bromoperoxidase could tolerate high temperatures.
(12) Pig kidney extracts have been partially purified by thermal denaturation and chromatography on Sephadex G-200 and D.E.A.E.
(13) RNA transcribed by RNA polymerase II on denatured DNA was only large RNA around 28S.
(14) The differential scanning calorimetry (DSC) studies demonstrate an increased susceptibility of the Ala 183----Pro mutant to thermal denaturation.
(15) The secreted antibody, which can be readily purified from the media without any denaturation or renaturation steps, retains antigen-binding activity.
(16) The electrophoretic pattern of free radical-exposed FABP was not markedly different when examined either by the non-denaturing or by denaturing PAGE, suggesting the absence of any degradation or aggregation of FABP by O2- or OH..
(17) Synthesis with denatured DNA as template presumably proceeds from 3'-hydroxyl termini formed at loop-back regions since the synthesized DNA product and template are covalently linked.
(18) Multiple contacts between the gamma-subunit and calmodulin (delta-subunit), as indicated by our data, may help to explain why strongly denaturing conditions are required to dissociate these two subunits, whereas complexes of calmodulin with most other target enzymes can be readily dissociated by merely lowering Ca2+ to submicromolar concentrations.
(19) Heating is shown to bring about only the denaturation of protein molecules in crystals.
(20) Renal tissues from two groups of patients were studied with fluorescein-labeled (Fl-) antibodies (Abs) to immunoglobulins, complement, and antibodies prepared in rabbits against BSA conjugate of 5-methyluridine (T) and cytidine (C), the latter two of which react specifically with denatured DNA.
Inherent
Definition:
(a.) Permanently existing in something; inseparably attached or connected; naturally pertaining to; innate; inalienable; as, polarity is an inherent quality of the magnet; the inherent right of men to life, liberty, and protection.
Example Sentences:
(1) This suggested that the chemical effects produced by shock waves were either absent or attenuated in the cells, or were inherently less toxic than those of ionizing irradiation.
(2) Even though attempts to generalize the data from childbearing women to women of childbearing age have an inherent conservative bias, the results of our study suggest that 988 women (95% CI 713 to 1336) aged 15 to 44 years in Quebec had HIV infection in 1989.
(3) In choosing between various scanning techniques the factors to be considered include availability, cost, the type of equipment, the expertise of the medical and technical staff, and the inherent capabilities of the system.
(4) Control incubations revealed an inherent difference between the two substrates; gram-positive supernatants consistently contained 5% radioactivity, whereas even at 0 h, those from the gram-negative mutant released 22%.
(5) The results strongly suggested that the rate of learning depended largely on factors inherent within the individual animals.
(6) These observations indicate that radiosensitivity is retained in vitro and is an inherent property of the testicular tumour cells.
(7) Principal conclusions are: 1) rapid change to predominantly heterosexual HIV transmission can occur in North America, with serious societal impact; 2) gender-specific clinical features can lead to earlier diagnosis of HIV infection in women; 3) HIV infection in women does not pursue an inherently more rapid course than that observed in men.
(8) The interresponse-time reinforcement contingencies inherent in these schedules may actually mask the effects of overall reinforcement rate; thus differences in response rate as a function of reinforcement rate when interresponse-time reinforcement is eliminated may be underestimated.
(9) In addition to the threat of industrial espionage to sustain this position, there is an inherent risk of Chinese equipment being used for intelligence purposes.
(10) Where Jim Broadbent stands as an inherently warm screen presence, his co-star's image is rather more flinty.
(11) Methodological difficulties inherent in incidence and prevalence studies of native Canadians are examined.
(12) Because of the inherent limitations of computed tomography in the visualization of posterior fossa structures, MR imaging should be considered the initial screening procedure in the assessment of patients with trigeminal neuralgia.
(13) It is shown that when a constant current is applied such that a stable equilibrium and rhythmic firing are present, the following predictions are inherent in the HH system of equations: (a) Small instantaneous voltage perturbations to the axon given at points along its firing spike result in phase resetting curves (when new phase versus old phase is plotted) with an average slope of 1.
(14) Continuous postoperative follow-up of the patients (from a few months to 14 years) and analysis of the early and late results allow to regard the combined technique of Coffey II-Nesbit-Goodwin as the method of choice having the slightest risk of peritonitis, intestinoureteral reflux and other complications inherent in other procedures.
(15) Psychiatric testimony to ultimate questions at law is limited by the inherent contextual variables of psychiatric clinical and experimental knowledge and practice.
(16) To study the inherent radiation sensitivity of vulvar carcinoma, we tested three new vulvar carcinoma cell lines and the long-established cell line A-431 by using a 96-well plate clonogenic assay, earlier shown by us to be suitable for survival studies of SCC.
(17) Numerous factors influenced its activity: method of spore production, inherent spore resistance characteristics, alkalination, storage time and storage temperature.
(18) Thus, many of the reported behavioral differences between normals and retardates of the same mental age are seen as products of motivational and experiential differences between these groups, rather than as the result of any inherent cognitive deficiency in the retardates.
(19) These data demonstrate that monocytes from subjects with psoriasis are altered and suggest an apparent inherent metabolic disorder.
(20) Hitherto performed abdominoperineal or sacroperineal procedures entailed major traumatizing surgery with an inherent risk of complications.