(n.) A small point or spot, made with a pen or other pointed instrument; a speck, or small mark.
(n.) Anything small and like a speck comparatively; a small portion or specimen; as, a dot of a child.
(v. t.) To mark with dots or small spots; as, to dot a line.
(v. t.) To mark or diversify with small detached objects; as, a landscape dotted with cottages.
(v. i.) To make dots or specks.
Example Sentences:
(1) We have developed a new procedure for the rapid preparation of undegraded total RNA from cultured cells for specific quantitation by dot blotting analysis.
(2) Analysis of glutamate dehydrogenase (GDH) expression by enzyme assay and immunoblots, as well as Northern and dot blots of poly (A)+ RNA, in the deletion strains indicates that there are two upstream regulatory sequences that control the level of gene expression.
(3) The method described uses film DOT-I and DOT-II by Dupont, whereby the exposure of the step wedge takes place on a linear accelerator with a photo energy of 10 MeV.
(4) The results indicated a very good comparability between the dot-blot assay and IF-tests, and this dot-blot method was ascertained as a simple and useful method for the scrub typhus serodiagnosis.
(5) Using a silver staining technique (AgNOR technique), we have investigated the nucleolar organizer-associated proteins (NORs) in formalin-fixed paraffin embedded conjunctival specimens of 15 intraepithelial squamous carcinomas, 10 hyperplastic-dysplastic samples and 10 control epithelial fragments; the mean number of intranuclear black dots was determined for each case.
(6) An increase in cytoplasmic PRL mRNA content was evident in all the animals treated with estrogen as revealed with cytoplasmic dot blot analysis.
(7) All these strains produced an enterotoxic principle, antigenically related to cholera coli family of enterotoxins, as detected by latex agglutination and immuno-dot-blot tests.
(8) ELISA, cDNA dot blot hybridization and transmission by vector aphids were used to investigate the occurrence and degree of cross-protection produced in oat plants by virus isolates representing five strains or serotypes of barley yellow dwarf virus, namely PAV, MAV, SGV, RPV and RMV.
(9) With anti-Go alpha antibodies, the immunofluorescence was more clearly focussed on a dotted pattern and the co-location with the voltage-dependent Ca2+ channel immunoreactivity indicates that both proteins were located in very close subcellular structures.
(10) Thresholds were measured for detecting perturbations in a regular lattice of dots by modulating local dot density, local dot luminance, or some combination of the two.
(11) As revealed by a dot immunobinding assay, the amount of immunoreactive bovine and human MFGM-associated antigen increased dramatically with the onset of lactation after delivery.
(12) All nine responders were negative for HBV DNA in serum by dot blot during or after treatment, but seven remained positive by polymerase chain amplification and Southern-blot hybridization.
(13) DNA sequencing and oligonucleotide dot-blot analysis of class II genes from two race-specific haplotypes indicate that susceptibility to IDDM is closely linked to the DQA1 locus and suggest that both the DQB1 (ref.
(14) The number of dots, the summed area of dots and the MF were separately evaluated by the "t" test.
(15) Hepatitis B virus DNA (HBV-DNA) in serum was studied in 67 anti-HBe patients using dot-blot hybridization, a modified technique and polymerase chain reaction (PCR).
(16) Dot Masr reporter Mohamed Abu Asay says in the past he could never report on the budget deficit in Egypt and the Saudi-Egyptian military operation in Yemen in the same piece.
(17) Nuclei from the normal mouse liver were partially digested with micrococcal nuclease, followed by DNA extraction, agarose gel electrophoresis and dot blot hybridization with 32P-labeled cDNA probes of CPS1 and ACT complex.
(18) Reconstituted flagellar filaments were demonstrated by three complementary methods: transmission electron microscopy, antigenic reactivity with H7 antiserum by a dot blot immunoassay, and immunogold localization of antiserum raised to the purified antigen to intact flagella on whole E. coli O157:H7.
(19) Rather, the sequences of alpha-amylase mRNA are rapidly degraded during heat shock as shown by in vitro translation and dot blot hybridization with a cDNA probe.
(20) Prism fixation disparity curves were determined in three different experimental situations: the routine method according to Ogle, a method to stimulate the synkinetic convergence (Experiment I, with one fixation point as sole binocular stimulus) and a method to stimulate the fusion mechanism (Experiment II, with random dot stereograms).
Vector
Definition:
(n.) Same as Radius vector.
(n.) A directed quantity, as a straight line, a force, or a velocity. Vectors are said to be equal when their directions are the same their magnitudes equal. Cf. Scalar.
Example Sentences:
(1) The dependence of fluorescence polarization of stained nerve fibres on the angle between the fibre axis and electrical vector of exciting light (azimuth characteristics) has been considered.
(2) A beta-adrenergic receptor cDNA cloned into a eukaryotic expression vector reliably induces high levels of beta-adrenergic receptor expression in 2-12% of COS cell colonies transfected with this plasmid after experimental conditions are optimized.
(3) We have constructed retroviral vectors derived from the genome of avian erythroblastosis virus ES4 (AEV ES4).
(4) The 6.6-kb DNA Bam HI fragment containing the resistance gene was cloned with the plasmid vector pIJ699.
(5) The plasmid pMucAMucB, constructed from the Haemophilus influenzae vector pDM2, and a similar plasmid, constructed from pBR322, increased the survival after UV irradiation of Escherichia coli AB1157 with the umu-36 mutation and also caused UV-induced mutation in the E. coli strain.
(6) In assessing damaged nets and curtains it must be recognised that anything less than the best vector control may have no appreciable impact on holoendemic malaria.
(7) When an expression vector containing plasminogen cDNA is transfected into baby hamster kidney cells, the number of drug-resistant colonies as well as the levels of plasminogen secreted by those colonies is lower than observed in similar transfections of other protease precursor genes.
(8) The vector is relatively small (6 kilobase pairs) and contains a portion of the L. seymouri alpha-tubulin gene positioned in-frame with a truncated neomycin phosphotransferase gene that confers resistance to the aminoglycoside G418.
(9) The presence of potential insect vectors and the occurrence of clinical signs are indications of active transmissions.
(10) Subcloning of pLR beta 118 into a transcription vector with subsequent in vitro transcription and translation using the reticulocyte lysate system in the presence of microsomes followed by immunoprecipitation with mAb OX6 and two-dimensional gel electrophoresis revealed the intact RT1.B beta I-chain.
(11) It is the most commonly reported vector-borne disease in the United States, where the incidence is highest in the eastern and midwestern states.
(12) The primary focus of the government at this point is vector control,” said Nadal.
(13) Transfer of the shuttle vectors from B. uniformis donors to E. coli occurred at the same frequencies when the matings were done aerobically or anaerobically.
(14) Discovery of this vectorhost-parasite system in the Americas, and the localization of promastigote flagellates (leptomonads) in the hindgut of the vector, should assist in clarifying interpretative problems associated with infection of wild-caught flies in studies on leishmaniasis in the Americas and elsewhere.
(15) ELISA, cDNA dot blot hybridization and transmission by vector aphids were used to investigate the occurrence and degree of cross-protection produced in oat plants by virus isolates representing five strains or serotypes of barley yellow dwarf virus, namely PAV, MAV, SGV, RPV and RMV.
(16) The results suggests that abrin B chain of chimeric protein may act as a vector to carry ACTI into the tumor cells.
(17) The peak biting activity of the vector and peak appearance of microfilariae in the peripheral blood occurred at about 01.00 h, which accounts for the optimum infection of the vector population.
(18) The transposon-containing plasmids were used to localize essential replication functions and to characterize the stability of large inserts in these vectors.
(19) A recombinant gene encoding human growth hormone (hGH) was stably introduced into cultured myoblasts with a retroviral vector.
(20) While it has been possible to readily produce large numbers of such templates from M13 or other single-stranded vectors for several years, the sequencing of double-stranded DNA templates using the ABI 373 DNA Sequencer has had a considerably lower success rate.