(1) At saturating concentrations of ER, plasmids bearing one, two, and four EREs in tandem bound approximately one, two, and four dimeric ER molecules, respectively.
(2) Interestingly, each of these fragments had a perfect palindromic estrogen responsive element (ERE) (GGT-CANNNTGACC).
(3) These data suggest that flanking DNA sequences may exert a significant effect on the activity of EREs as hormone-dependent transcription activators.
(4) In 11 spontaneously breathing patients with chronic obstructive pulmonary disease (COPD) in acute ventilatory failure, we measured the total inspiratory (WItot) and total resistive (WI + Eres) work rate of breathing, together with lung mechanics (dynamic pulmonary elastance and inspiratory and expiratory pulmonary flow resistance).
(5) This element binds to the estrogen receptor in vitro as assessed by gel retardation assay similar to the vitellogenin gene ERE.
(6) The two polypeptides are different as judged by peptide mapping, and only the 85-kDa polypeptide can be cross-linked to the bromodeoxyuridine-substituted synthetic ERE by UV irradiation.
(7) Two methods have been suggested for the calculation of pHapp and the loss of activity on particle preparation, these methods are based on the use of the ERE in conjunction with experimental data.
(8) These results suggest that the ability of ER and TR to functionally discriminate between an ERE and a TRE is a result of dimerization of their DBDs.
(9) The presence of the biologically active hER is confirmed in co-injection experiments, in which HEO is co-introduced with a CAT reporter gene under the control of a vitellogenin promoter containing or lacking the ERE.
(10) Gene transfer experiments using estrogen-responsive cells have shown that the 13 bp perfect palindromic element GGTCACTGTGACC found upstream of the Xenopus laevis vitellogenin gene A2 promoter mediates hormonal stimulation and thus, was called the estrogen-responsive element (ERE).
(11) The ovalbumin promoter half-palindromic ERE motif located close to the TATA box was required for the activity of the distal DH3 ERE, but could be replaced by the binding sites of other transactivators.
(12) The equilibrium constant for the redox equilibration of fatty acid synthase in a glutathione redox buffer is 15 mM (Ered + GSSG in equilibrium Eox + 2GSH).
(13) When sequenced, these elements showed remarkable diversity and were different from the consensus vitellogenin A2 ERE.
(14) However at 32 degrees C Ta, there was an increase in rectal temperature in response to haloperidol application; this hyperthermia was due to a decrease in both the ear blood flow and respiratory evaporative heat loss (Eres).
(15) The affinity of estradiol binding to receptors was reduced only 2- and 5-fold, respectively, in the double and quadruple Cys to Ala mutants, and estradiol was an effective stimulator of transcription from an estrogen-responsive reporter gene [(ERE)2-TATA-CAT].
(16) A stimulatory estrogen response element (ERE) was localized to a 32-bp region between -547 and -516 bp.
(17) In these genes, two ERE homologues, which have only low, if any, regulatory capacity on their own, act synergistically to achieve high estrogen inducibility.
(18) "Gnnmph, I can't 'ave it 'ere, I 'aven't 'ad my enema," wails a labouring housewife, straining fruitlessly on a communal tenement bog as horrified neighbours look on in their rollers.
(19) This ERE also mediated down-regulation by progestins in the presence of the progesterone receptor, even though it has no progesterone receptor binding ability.
(20) This 67 bp region contains a consensus for the core sequence of the glucocorticoid responsive element (GRE) and the estrogen responsive element (ERE).
Ern
Definition:
(n.) Alt. of Erne
(v. i.) To stir with strong emotion; to grieve; to mourn. [Corrupted into yearn in modern editions of Shakespeare.]
Example Sentences:
(1) The synthetic ester cyclopentylpropionate, like E2, produced a rapid ERn response and a significantly shorter uterotropic response than the stearate ester.
(2) The leaders of the world's eight wealthiest countries, including Russian president Vladimir Putin and German chancellor Angela Merkel, are due to meet at the luxury Lough Erne resort in Co Fermanagh for the conference on 17-18 June.
(3) Gerald Grosvenor came into the line of succession only because the 3rd Duke was childless and the title passed to a cousin, who became 4th Duke in 1963 and then, when he died four years later, to his younger brother, Gerald’s father, Robert Grosvenor, who farmed in Northern Ireland and lived on an island in Lough Erne.
(4) The oligomeric ERc eluted as a single, sharp peak near the exclusion volume of the gel column; ERn eluted as a broad peak.
(5) Reductions in total ER (ERn + ERc) were sufficient to account for all reductions and altered dynamics of ERn, except for the delayed attainment of peak ERn in UT.
(6) The correlation coefficients for ERc, ERn and ERc+n were 0.960, 0.980 and 0.950, respectively.
(7) We must work together to keep this hope alive, as we agreed to at the Group of 8 meeting in Lough Erne in Northern Ireland in June, and steer the discussion back toward negotiations.
(8) This unoccupied nuclear ER (ERn) whose hormone binding ability was extremely thermostable could be extracted with 0.4 M KCl.
(9) "One of highlights, says Starks, was launching the institute's open data certificate at June's G8 meeting in Lough Erne, where the themes were tax, transparency and trade.
(10) We measured the uterotropic response and the formation of uterine nuclear estrogen receptors (ERn) produced by iv administration of a representative ester, E2-17-stearate, in comparison to E2, other natural C-17 conjugates of E2, E2-17-glucuronide, and E2-17-sulfate, and the pharmacological ester E2-17-cyclopentylpropionate.
(11) [Erne, D., Sargent, D. F., & Schwyzer, R. (1985) Biochemistry 24, 4261-4263].
(12) In the tumours of postmenopausal women an inverse significant correlation was demonstrated between the concentrations of EGFR vs. ERc, ERn, and PRc while no such correlation was noted in the tumours of premenopausal women.
(13) 2,3,7,8-TCDD causes a dose-dependent decrease in uterine ERc, ERn, PRc, and PRn levels which persist up to 7 days.
(14) Using a hydroxylapatite exchange method for ER, little or no nuclear ER (ERN) could be detected, but with the EIA both cytosolic (ERC) and ERN were detected in almost all specimens, although in meager concentrations.
(15) Nuclear estrogen receptors (ERn) can now be reliably analyzed using the monoclonal estrogen receptor enzyme immunoassay.
(16) In these studies we also examined the changes which occur in estrogen nuclear (ERn) and progestin cytosol (PRc) receptor concentrations in the preoptic area (POA), medial basal hypothalamus (MBH), corticomedial amygdala (CMA) and pituitary gland (PIT) associated with these physiological responses.
(17) In the control pituitary nuclei, 70% of ERn were in the salt-soluble fraction, of which the great majority were occupied by endogenous steroid.
(18) Both the heat-transformed cytosolic estrogen receptor, ERC*, and a major fraction of the estrogen receptor extracted from nuclei, ERN, contained two sites for H165, but only one for H222.
(19) The oestradiol nuclear receptors (ERn) followed the same pattern in the 3 sampling areas.
(20) In the untreated BPH group, ER were higher in the n than in the c fraction: ERn were positive in 14 cases and ERc in 12 of 17 cases.