(1) At saturating concentrations of ER, plasmids bearing one, two, and four EREs in tandem bound approximately one, two, and four dimeric ER molecules, respectively.
(2) Interestingly, each of these fragments had a perfect palindromic estrogen responsive element (ERE) (GGT-CANNNTGACC).
(3) These data suggest that flanking DNA sequences may exert a significant effect on the activity of EREs as hormone-dependent transcription activators.
(4) In 11 spontaneously breathing patients with chronic obstructive pulmonary disease (COPD) in acute ventilatory failure, we measured the total inspiratory (WItot) and total resistive (WI + Eres) work rate of breathing, together with lung mechanics (dynamic pulmonary elastance and inspiratory and expiratory pulmonary flow resistance).
(5) This element binds to the estrogen receptor in vitro as assessed by gel retardation assay similar to the vitellogenin gene ERE.
(6) The two polypeptides are different as judged by peptide mapping, and only the 85-kDa polypeptide can be cross-linked to the bromodeoxyuridine-substituted synthetic ERE by UV irradiation.
(7) Two methods have been suggested for the calculation of pHapp and the loss of activity on particle preparation, these methods are based on the use of the ERE in conjunction with experimental data.
(8) These results suggest that the ability of ER and TR to functionally discriminate between an ERE and a TRE is a result of dimerization of their DBDs.
(9) The presence of the biologically active hER is confirmed in co-injection experiments, in which HEO is co-introduced with a CAT reporter gene under the control of a vitellogenin promoter containing or lacking the ERE.
(10) Gene transfer experiments using estrogen-responsive cells have shown that the 13 bp perfect palindromic element GGTCACTGTGACC found upstream of the Xenopus laevis vitellogenin gene A2 promoter mediates hormonal stimulation and thus, was called the estrogen-responsive element (ERE).
(11) The ovalbumin promoter half-palindromic ERE motif located close to the TATA box was required for the activity of the distal DH3 ERE, but could be replaced by the binding sites of other transactivators.
(12) The equilibrium constant for the redox equilibration of fatty acid synthase in a glutathione redox buffer is 15 mM (Ered + GSSG in equilibrium Eox + 2GSH).
(13) When sequenced, these elements showed remarkable diversity and were different from the consensus vitellogenin A2 ERE.
(14) However at 32 degrees C Ta, there was an increase in rectal temperature in response to haloperidol application; this hyperthermia was due to a decrease in both the ear blood flow and respiratory evaporative heat loss (Eres).
(15) The affinity of estradiol binding to receptors was reduced only 2- and 5-fold, respectively, in the double and quadruple Cys to Ala mutants, and estradiol was an effective stimulator of transcription from an estrogen-responsive reporter gene [(ERE)2-TATA-CAT].
(16) A stimulatory estrogen response element (ERE) was localized to a 32-bp region between -547 and -516 bp.
(17) In these genes, two ERE homologues, which have only low, if any, regulatory capacity on their own, act synergistically to achieve high estrogen inducibility.
(18) "Gnnmph, I can't 'ave it 'ere, I 'aven't 'ad my enema," wails a labouring housewife, straining fruitlessly on a communal tenement bog as horrified neighbours look on in their rollers.
(19) This ERE also mediated down-regulation by progestins in the presence of the progesterone receptor, even though it has no progesterone receptor binding ability.
(20) This 67 bp region contains a consensus for the core sequence of the glucocorticoid responsive element (GRE) and the estrogen responsive element (ERE).
Erstwhile
Definition:
(adv.) Till then or now; heretofore; formerly.
Example Sentences:
(1) Alex Turner has already set about ingratiating himself with the 2013 festival by guesting with his erstwhile partner in the Last Shadow Puppets, Miles Kane, earlier this afternoon, but as he takes to the Pyramid Stage for the Monkeys' headline slot, piling straight into the bluesy electronic throbs of new single Do I Wanna Know in a sharp striped suit and teddy quiff and throwing the odd karate beckoning motion, there's a real sense of points to be proved.
(2) It's as well to be aware of the beckoning avenues of justification that are drawing in so many of our erstwhile comrades.
(3) That seemed not to worry Unite's Len McCluskey, his erstwhile blustery critic, who sent out paeans of reckless praise: "This is a tour de force … the best speech from a Labour leader I have heard."
(4) Appropriately for a presenter steeped in statistics, the BBC's erstwhile economics editor drew up a cost-benefit analysis weighing up the pros and cons of replacing Jeremy Paxman as Newsnight's lead anchor, to little effect.
(5) The erstwhile envoy caused ructions earlier this week when he declared in an interview with the New York Times that Greece's IMF-dicated fiscal adjustment program was doomed to failure.
(6) It was all very well for erstwhile broadsheet newspaper readers to jeer "Who cares?"
(7) When George Robertson, erstwhile secretary general of Nato, used a New York speech to warn of the "cataclysmic" effects of a yes vote, suggesting it would be an early Christmas present for global terrorists, even close colleagues had to avert their ears.
(8) Blackout will be organising grassroots events, nationwide, for people to come out and show their solidarity in the fight for equal human rights.” Backers include Fruitvale Station actor Michael B Jordan, Vampire Diaries star Kat Graham, hiphop mogul Russell Simmons and erstwhile Rage Against the Machine guitarist Tom Morello.
(9) He has also covered an old Scottish folk song, 'Hymn Of The Whale', as well as a fearsome semi-industrial track in the company of the erstwhile drummer from Nine Inch Nails.
(10) But if he can roll out enough insults to Democrats and pal around with more conventional Tea Party heroes such as Steve Lonegan (the erstwhile GOP NJ senate candidate), Christie could convince the conservative base he has their best interests at his large heart.
(11) If its officials have been more visible it is a function of the fact that many, including Koussa and his erstwhile deputy, Khaled Kaim, have more experience in dealing with the west.
(12) For the erstwhile parliamentarian, who has had more time on his hands since losing his Morley and Outwood seat at the general election in May, it is an opportunity to indulge a passion for the club he has harboured since he was a small boy.
(13) Earlier this month, the Kabul-based military command, under marine general Joseph Dunford, sharply rebuked its erstwhile Afghanistan partners for releasing 65 detainees it said had US and Afghan blood on their hands.
(14) Archer's creepy Pecksniffian pals If there's one thing worse than Jeffrey Archer, it is the erstwhile friends of Jeffrey Archer.
(15) Moreover, after what’s sure to be millions of people who were otherwise not very engaged in the topic of internet security go ahead and read Zuckerberg’s post, they may care just a bit more about what’s being done to us all by our erstwhile protectors – not just by the criminals.
(16) Although still stunned by the Fifa president’s resignation, Dyke thinks he can explain it, starting with the allegations of a bribe to erstwhile Fifa official Jack Warner.
(17) For more than two weeks, his erstwhile deputies claimed Mehsud was ill while they arranged his succession.
(18) Harassed western alliance seeks erstwhile head of Taliban administration.
(19) That is our project, and we are getting there.” Manchester United reverted to the three-centre-back system they started out with under Louis van Gaal for their visit to the erstwhile league leaders, partly out of respect for Vardy’s hot streak but also because Marcos Rojo had to pull out injured.
(20) The successful cloning of a non-structural antigen from the genome of what is now designated as the 'hepatitis C virus' (HCV) has transformed an erstwhile diagnosis of exclusion for non-A, non-B hepatitis (NANBH).