(1) At saturating concentrations of ER, plasmids bearing one, two, and four EREs in tandem bound approximately one, two, and four dimeric ER molecules, respectively.
(2) Interestingly, each of these fragments had a perfect palindromic estrogen responsive element (ERE) (GGT-CANNNTGACC).
(3) These data suggest that flanking DNA sequences may exert a significant effect on the activity of EREs as hormone-dependent transcription activators.
(4) In 11 spontaneously breathing patients with chronic obstructive pulmonary disease (COPD) in acute ventilatory failure, we measured the total inspiratory (WItot) and total resistive (WI + Eres) work rate of breathing, together with lung mechanics (dynamic pulmonary elastance and inspiratory and expiratory pulmonary flow resistance).
(5) This element binds to the estrogen receptor in vitro as assessed by gel retardation assay similar to the vitellogenin gene ERE.
(6) The two polypeptides are different as judged by peptide mapping, and only the 85-kDa polypeptide can be cross-linked to the bromodeoxyuridine-substituted synthetic ERE by UV irradiation.
(7) Two methods have been suggested for the calculation of pHapp and the loss of activity on particle preparation, these methods are based on the use of the ERE in conjunction with experimental data.
(8) These results suggest that the ability of ER and TR to functionally discriminate between an ERE and a TRE is a result of dimerization of their DBDs.
(9) The presence of the biologically active hER is confirmed in co-injection experiments, in which HEO is co-introduced with a CAT reporter gene under the control of a vitellogenin promoter containing or lacking the ERE.
(10) Gene transfer experiments using estrogen-responsive cells have shown that the 13 bp perfect palindromic element GGTCACTGTGACC found upstream of the Xenopus laevis vitellogenin gene A2 promoter mediates hormonal stimulation and thus, was called the estrogen-responsive element (ERE).
(11) The ovalbumin promoter half-palindromic ERE motif located close to the TATA box was required for the activity of the distal DH3 ERE, but could be replaced by the binding sites of other transactivators.
(12) The equilibrium constant for the redox equilibration of fatty acid synthase in a glutathione redox buffer is 15 mM (Ered + GSSG in equilibrium Eox + 2GSH).
(13) When sequenced, these elements showed remarkable diversity and were different from the consensus vitellogenin A2 ERE.
(14) However at 32 degrees C Ta, there was an increase in rectal temperature in response to haloperidol application; this hyperthermia was due to a decrease in both the ear blood flow and respiratory evaporative heat loss (Eres).
(15) The affinity of estradiol binding to receptors was reduced only 2- and 5-fold, respectively, in the double and quadruple Cys to Ala mutants, and estradiol was an effective stimulator of transcription from an estrogen-responsive reporter gene [(ERE)2-TATA-CAT].
(16) A stimulatory estrogen response element (ERE) was localized to a 32-bp region between -547 and -516 bp.
(17) In these genes, two ERE homologues, which have only low, if any, regulatory capacity on their own, act synergistically to achieve high estrogen inducibility.
(18) "Gnnmph, I can't 'ave it 'ere, I 'aven't 'ad my enema," wails a labouring housewife, straining fruitlessly on a communal tenement bog as horrified neighbours look on in their rollers.
(19) This ERE also mediated down-regulation by progestins in the presence of the progesterone receptor, even though it has no progesterone receptor binding ability.
(20) This 67 bp region contains a consensus for the core sequence of the glucocorticoid responsive element (GRE) and the estrogen responsive element (ERE).
Ewe
Definition:
(n.) The female of the sheep, and of sheeplike animals.
Example Sentences:
(1) An experimental model was established in the ewe allowing one to predict with accuracy an antral follicle that coincidentally would either undergo ovulation (6-8 mm diameter) or atresia (3-4 mm diameter) following synchronization of luteal regression and the onset of the gonadotropin surge.
(2) Lambing rates approach 1.5 lambs per ewe per year, but a death rate of 23 per cent and an offtake of 27 per cent, means that flock numbers are probably slightly declining.
(3) Only one ewe aborted, 10 days after the first infecting dose, at 94 days of gestation; L monocytogenes was isolated from several sites in both its aborted fetuses.
(4) In the water-loaded state, MAP rose significantly at the lowest rate of infusion in both pregnant and non-pregnant ewes.
(5) Three of the tumours represented primary soft tissue lesions, while locally recurrent tumour or pulmonary metastases were studied from the 4 skeletal tumours, all of which had been diagnosed previously as Ewing's sarcomas.
(6) In this ewe, and in 4 of 7 other sheep diagnosed as having abomasal emptying defects, aspartate transaminase and sorbitol dehydrogenase activities were high, and histopathologic evidence of hepatic congestion and ischemia was found.
(7) Similar sponges were reintroduced into four ewes at each of the intervals 1, 3, 5, and 7 days later; three ewes served as controls.
(8) Synthetic LHRH administered to ewes by intravenous infusion or subcutaneous injection was readily detectable in peripheral plasma.
(9) The effect of age of the ewe and pregnancy on concentrations of plasma calcium, phosphorus and magnesium and its relationship to the bent-leg syndrome in lambs, were investigated.
(10) After surgical resection the facial mass was diagnosed as Ewing's sarcoma histopathologically.
(11) Although a PGF2 alpha analogue (Lutalyse) infusion into the uterine vein of two ewes with persistent CLs failed to induced luteolysis, it did stimulate a large release of OT into the UOV.
(12) Seven lactating Lacaune ewes underwent either a total luteectomy on day 19 of pregnancy (D19) (compensated from that stage by a daily progesterone supplementation of 25 mg to ensure embryonic survival; group 1:4 animals) or a control laparotomy (group 2: 3 animals).
(13) Faecal samples of the Romanov ewes more often harboured Nematodirus eggs while the larvae recovered from cultures of these samples contained a higher percentage of Teladorsagia.
(14) In experiment 2, antibodies (Ab) were extracted from sera obtained from experiment 1 ewes and then were injected i.v.
(15) Serologically the aborted ewes were positive for brucellosis by one or more tests.
(16) Children with osteosarcoma or Ewing's sarcoma rarely have bone disease distant from the site of their primary bone lesion at presentation.
(17) The androgenized ewes showed poorer oestrous responses to each hormone although rams showed interest in the ewes.
(18) Plasma melatonin concentrations were higher during the hours of darkness in the ewes and fetuses in both the normal and altered groups; i.e.
(19) Four of these ewes received a second increase in GnRH pulse frequency, every 2 h and hourly on the subsequent 2 days.
(20) Ewing's sarcoma is considered a primary malignant tumor of bone.