(1) At saturating concentrations of ER, plasmids bearing one, two, and four EREs in tandem bound approximately one, two, and four dimeric ER molecules, respectively.
(2) Interestingly, each of these fragments had a perfect palindromic estrogen responsive element (ERE) (GGT-CANNNTGACC).
(3) These data suggest that flanking DNA sequences may exert a significant effect on the activity of EREs as hormone-dependent transcription activators.
(4) In 11 spontaneously breathing patients with chronic obstructive pulmonary disease (COPD) in acute ventilatory failure, we measured the total inspiratory (WItot) and total resistive (WI + Eres) work rate of breathing, together with lung mechanics (dynamic pulmonary elastance and inspiratory and expiratory pulmonary flow resistance).
(5) This element binds to the estrogen receptor in vitro as assessed by gel retardation assay similar to the vitellogenin gene ERE.
(6) The two polypeptides are different as judged by peptide mapping, and only the 85-kDa polypeptide can be cross-linked to the bromodeoxyuridine-substituted synthetic ERE by UV irradiation.
(7) Two methods have been suggested for the calculation of pHapp and the loss of activity on particle preparation, these methods are based on the use of the ERE in conjunction with experimental data.
(8) These results suggest that the ability of ER and TR to functionally discriminate between an ERE and a TRE is a result of dimerization of their DBDs.
(9) The presence of the biologically active hER is confirmed in co-injection experiments, in which HEO is co-introduced with a CAT reporter gene under the control of a vitellogenin promoter containing or lacking the ERE.
(10) Gene transfer experiments using estrogen-responsive cells have shown that the 13 bp perfect palindromic element GGTCACTGTGACC found upstream of the Xenopus laevis vitellogenin gene A2 promoter mediates hormonal stimulation and thus, was called the estrogen-responsive element (ERE).
(11) The ovalbumin promoter half-palindromic ERE motif located close to the TATA box was required for the activity of the distal DH3 ERE, but could be replaced by the binding sites of other transactivators.
(12) The equilibrium constant for the redox equilibration of fatty acid synthase in a glutathione redox buffer is 15 mM (Ered + GSSG in equilibrium Eox + 2GSH).
(13) When sequenced, these elements showed remarkable diversity and were different from the consensus vitellogenin A2 ERE.
(14) However at 32 degrees C Ta, there was an increase in rectal temperature in response to haloperidol application; this hyperthermia was due to a decrease in both the ear blood flow and respiratory evaporative heat loss (Eres).
(15) The affinity of estradiol binding to receptors was reduced only 2- and 5-fold, respectively, in the double and quadruple Cys to Ala mutants, and estradiol was an effective stimulator of transcription from an estrogen-responsive reporter gene [(ERE)2-TATA-CAT].
(16) A stimulatory estrogen response element (ERE) was localized to a 32-bp region between -547 and -516 bp.
(17) In these genes, two ERE homologues, which have only low, if any, regulatory capacity on their own, act synergistically to achieve high estrogen inducibility.
(18) "Gnnmph, I can't 'ave it 'ere, I 'aven't 'ad my enema," wails a labouring housewife, straining fruitlessly on a communal tenement bog as horrified neighbours look on in their rollers.
(19) This ERE also mediated down-regulation by progestins in the presence of the progesterone receptor, even though it has no progesterone receptor binding ability.
(20) This 67 bp region contains a consensus for the core sequence of the glucocorticoid responsive element (GRE) and the estrogen responsive element (ERE).
Malkin
Definition:
(n.) Originally, a kitchenmaid; a slattern.
(n.) A mop made of clouts, used by the kitchen servant.
(n.) A scarecrow.
(n.) A mop or sponge attached to a jointed staff for swabbing out a cannon.
Example Sentences:
(1) Michelle Malkin, a conservative blogger, tweeted that the moderator was wrong to have backed an "Obama lie" over Libya.
(2) Bonnie Malkin The Netherlands: Car trouble At one point on this holiday we visited the Vaalserberg, a place where the borders of Netherlands, Belgium and Germany meet.
(3) Acta 430, 538-547; Malkin & Bearden (1976) FEBS Lett.
(4) This transient has been ascribed to a back-reaction of the two primary reagents of Photosystem II (Malkin, R. and Bearden, A.J.
(5) We propose that these signals are due to bulk chlorophyll oxidation and not, as was previously thought [Knaff & Malkin (1973) Arch.
(6) He comes from the most vulnerable group in America: wealthy old white males.” Nehlen also earned the backing of arch-conservative heroines Sarah Palin, Ann Coulter and Michelle Malkin, as well as Tea Party activists hoping for a repeat of the primary surprise that ousted then House majority leader Eric Cantor.
(7) So Republicans should be thankful for a coinage by the conservative commentator Michelle Malkin, " hate couture ", which refers to the scandalous fact that people in the fashion industry tend to vote Democratic, and that Diane von Furstenberg made a joke at a recent event about how Republicans weren't allowed.
(8) This defeat was painful for the core of Sidney Crosby, Evgeni Malkin and coach Dan Bylsma that seemed pointed toward a dynasty after winning the 2009 Stanley Cup.
(9) The primary structure and cotranscription of the petCA genes encoding the Rieske-FeS (nuclear encoded in plants) and apocytochrome f proteins has been described previously (Kallas, T., Spiller, S., and Malkin, R. (1988) Proc.
(10) (Some other members of that club: Michelle Malkin, Bill Maher and occasionally Ann Coulter, depending on Bill’s mood.)
(11) Mathematical analysis suggests that if only two forms of Q participate beyond I, then system I action is required for D. If three forms participate, then the system Q --> QH --> Q' (see text) may explain D. The Malkin model (14), in its present form, does not allow D.
(12) Such changes in relaxation time can account for the different quantitative conclusions incorrectly arrived at from measurements made at saturating microwave powers [Bearden & Malkin (1976) Biochem.
(13) The Rieske protein of the ubiquinol-cytochrome c oxidoreductase (bc1 complex or b6f complex) contains a [2Fe-2S] cluster which is thought to be bound to the protein via two nitrogen and two sulfur ligands [Britt, R. D., Sauer, K., Klein, M. P., Knaff, D. B., Kriauciunas, A., Yu, C.-A., Yu, L., & Malkin, R. (1991) Biochemistry 30, 1892-1901; Gurbiel, R. J., Ohnishi, T., Robertson, D. E., Daldal, F., & Hoffman, B. M. (1991) Biochemistry 30, 11579-11584].
(14) Ben Carson and Ted Cruz, appearing along with prominent conservative figures like Mark Levin, Sean Hannity and Michelle Malkin, emphasized their hard-right bona fides.