(1) At saturating concentrations of ER, plasmids bearing one, two, and four EREs in tandem bound approximately one, two, and four dimeric ER molecules, respectively.
(2) Interestingly, each of these fragments had a perfect palindromic estrogen responsive element (ERE) (GGT-CANNNTGACC).
(3) These data suggest that flanking DNA sequences may exert a significant effect on the activity of EREs as hormone-dependent transcription activators.
(4) In 11 spontaneously breathing patients with chronic obstructive pulmonary disease (COPD) in acute ventilatory failure, we measured the total inspiratory (WItot) and total resistive (WI + Eres) work rate of breathing, together with lung mechanics (dynamic pulmonary elastance and inspiratory and expiratory pulmonary flow resistance).
(5) This element binds to the estrogen receptor in vitro as assessed by gel retardation assay similar to the vitellogenin gene ERE.
(6) The two polypeptides are different as judged by peptide mapping, and only the 85-kDa polypeptide can be cross-linked to the bromodeoxyuridine-substituted synthetic ERE by UV irradiation.
(7) Two methods have been suggested for the calculation of pHapp and the loss of activity on particle preparation, these methods are based on the use of the ERE in conjunction with experimental data.
(8) These results suggest that the ability of ER and TR to functionally discriminate between an ERE and a TRE is a result of dimerization of their DBDs.
(9) The presence of the biologically active hER is confirmed in co-injection experiments, in which HEO is co-introduced with a CAT reporter gene under the control of a vitellogenin promoter containing or lacking the ERE.
(10) Gene transfer experiments using estrogen-responsive cells have shown that the 13 bp perfect palindromic element GGTCACTGTGACC found upstream of the Xenopus laevis vitellogenin gene A2 promoter mediates hormonal stimulation and thus, was called the estrogen-responsive element (ERE).
(11) The ovalbumin promoter half-palindromic ERE motif located close to the TATA box was required for the activity of the distal DH3 ERE, but could be replaced by the binding sites of other transactivators.
(12) The equilibrium constant for the redox equilibration of fatty acid synthase in a glutathione redox buffer is 15 mM (Ered + GSSG in equilibrium Eox + 2GSH).
(13) When sequenced, these elements showed remarkable diversity and were different from the consensus vitellogenin A2 ERE.
(14) However at 32 degrees C Ta, there was an increase in rectal temperature in response to haloperidol application; this hyperthermia was due to a decrease in both the ear blood flow and respiratory evaporative heat loss (Eres).
(15) The affinity of estradiol binding to receptors was reduced only 2- and 5-fold, respectively, in the double and quadruple Cys to Ala mutants, and estradiol was an effective stimulator of transcription from an estrogen-responsive reporter gene [(ERE)2-TATA-CAT].
(16) A stimulatory estrogen response element (ERE) was localized to a 32-bp region between -547 and -516 bp.
(17) In these genes, two ERE homologues, which have only low, if any, regulatory capacity on their own, act synergistically to achieve high estrogen inducibility.
(18) "Gnnmph, I can't 'ave it 'ere, I 'aven't 'ad my enema," wails a labouring housewife, straining fruitlessly on a communal tenement bog as horrified neighbours look on in their rollers.
(19) This ERE also mediated down-regulation by progestins in the presence of the progesterone receptor, even though it has no progesterone receptor binding ability.
(20) This 67 bp region contains a consensus for the core sequence of the glucocorticoid responsive element (GRE) and the estrogen responsive element (ERE).
Ure
Definition:
(n.) The urus.
(n.) Use; practice; exercise.
(v. t.) To use; to exercise; to inure; to accustom by practice.
Example Sentences:
(1) In addition, the URE appears to play a role in promoting the replication of polyoma DNA as determined through two different experimental approaches.
(2) Each open reading frame was preceded by a ribosome-binding site, with the exception of ureE.
(3) The results of short-term tests (uring adriamycin, daunorubicin, and dactinomycin) in roughly 100 human tumors were compared with data in the literature on therapy with the same cytostatic agents.
(4) However, in females exposed to female uring during the first 7 days of VC this effect was absent.
(5) It starts to feel like it’s a process where if you give money you solve the problem, and really sometimes giving money creates another problem.” When he was told there was just one African-born performer on the track, he said: “That’s great, just a few more would be nice and also maybe go there – all those people who are making that.” Ultravox’s Midge Ure said the song was by no means a masterpiece, but is more about getting people as engaged with the fight against Ebola as they were in 1984, when a total of £8m was raised.
(6) Diagnosis depends on detection of persistent bacteriuria by careful screening and culture of properly collected uring specimens.
(7) The rIL-2 infusion caused a reversible fall in ures and a non-reversible rise in creatinine.
(8) The experimental evidence suggests that either the repression associated with the URE sequence is mediated by a direct, one-to-one interaction between the proteins recognizing the URE and GCRE, or alternatively, that there is a direct interaction between the activator and repressor for a general transcription factor.
(9) Furthermore, repression is seen when the URE is separated from the UAS by up to 214 bp.
(10) This suggests that either a different accessory element and cognate protein interacts with the horse URE to provide placenta-specific expression or that a completely different set of regulatory elements is required for placenta-specific expression in horses.
(11) A polyacrylamide gel electrophoresis, without sodium dodecyl sulfate-uree, of the total serum allowed after incubation with the N-acetyl-beta-D-glucosaminide (6-bromo-2-hydroxy-3-naphthoyl-O-anisidine) coupled with the diazonium salt of O-amino-azotoluene to localise two zones of enzymatic activity.
(12) The predicted UreE and UreG polypeptides exhibit some degree of similarity with the respective polypeptides encoded by the accessory genes of the Klebsiella aerogenes urease operon (33 and 92% similarity, respectively, taking into account conservative amino acid changes), whereas this homology was restricted to a domain of the UreF polypeptide (44% similarity for the last 73 amino acids of the K. aerogenes UreF polypeptide).
(13) We have cloned ivoB which codes for a conidiophore-specific phenol oxidase (AHTase) via the adjacent selectable ureD gene.
(14) Nuclear proteins extracted from JEG-3 choriocarcinoma cells bind specifically to oligonucleotides corresponding to both the URE and CRE domains as well as to a downstream domain (-99 to -72) that contains consensus CCAAT motifs on both the sense and antisense strands.
(15) Thus, one or more of the ureE, ureF, or ureG gene products are involved in nickel incorporation into urease.
(16) Adult DDE and URE were induced to express seminal cytodifferentiation and produced the complete spectrum of major seminal vesicle secretory (SVS) proteins.
(17) Perinatal exposure of mice to URE has been found to result in increased tumor induction compared to exposure of adult animals.
(18) In transient expression assays, the upstream and downstream domains of the URE were shown to independently enhance CRE-mediated transcription of the alpha gene.
(19) Binding to this multisite DNA fragment is readily disrupted using the URE sequence, but not the CRE sequence as a competitor, suggesting that the URE binding factor may stabilize DNA-protein interactions in these adjacent complexes.
(20) This new regulatory element is highly conserved across species and contains a palindromic binding site for a 50-kDa nuclear factor(s) which is distinct from the factors that bind the URE, CRE, and CCAAT box of the alpha subunit gene.