(n.) A shark of the genus Sphyrna or Zygaena, having the eyes set on projections from the sides of the head, which gives it a hammer shape. The Sphyrna zygaena is found in the North Atlantic. Called also hammer fish, and balance fish.
(n.) A fresh-water fish; the stone-roller.
(n.) An African fruit bat (Hypsignathus monstrosus); -- so called from its large blunt nozzle.
Example Sentences:
(1) Six additional divalent ions were tested for their ability to support hammerhead cleavage.
(2) However, a segment of approximately one-third of the PLMVd sequence has the elements required to form in the RNAs of both polarities the hammerhead structures proposed to act in the in vitro self-cleavage of avocado sunblotch viroid (ASBVd) and some satellite RNAs.
(3) When injected into the nucleus of frog oocytes, the ribozyme tRNA gene (ribtDNA) produces 'hammerhead' ribozymes which cleave the 5' sequences of U7snRNA, its target substrate, with high efficiency in vitro.
(4) All combinations of mutant substrate and mutant ribozyme were less active than the corresponding single mutations, suggesting that the hammerhead contains few, if any, replaceable tertiary interactions as are found in tRNA.
(5) Nine different hammerhead RNA self-cleaving domains consistent with the consensus secondary structure proposed by Keese and Symons (1987) were prepared and tested for cleavage.
(6) To investigate the binding properties of Mg2+ to the hammerhead ribozyme, cleavage rates and CD spectra for substrates containing inosine or guanosine at the cleavage site were measured.
(7) The hammerhead domains consist of a 34 nucleotide ribozyme bound to a complementary 13 nucleotide non-cleavable DNA substrate.
(8) Also, inversion of configuration at phosphorus is confirmed for a two-stranded hammerhead.
(9) Based on comparisons with self-cleaving plant viral satellite RNAs, hammerhead-shaped active structures, each containing one self-cleavage site, were proposed for the plus and minus ASBV RNAs and the newt RNA, but the stability of these hammerheads has been questioned.
(10) Here, we show that the purified full-length dimeric plus RNA, when incubated under our standard self-cleavage conditions, also self-cleaved by a double-hammerhead structure.
(11) The hammerhead ribozyme, as engineered by J. Haseloff and W. L. Gerlach [(1988) Nature (London) 334, 585-591], is an RNA molecule containing two regions of conserved nucleotides, a double helix, called helix II, which connects the two conserved regions, and flanking arms of variable sequence, which hybridize the ribozyme to its specific target.
(12) Insertion, deletion and base substitution mutations were carried out on a 58 base RNA containing the sequence of the single-hammerhead structure of the plus RNA of the virusoid of lucerne transient streak virus, and the effects on self-cleavage assessed.
(13) Analysis of the cleavage products of several of these hammerhead analogues confirms the involvement in the reaction of the 2'-OH adjacent to the cleavage site in the substrate, and demonstrates that some 2'-OH groups in the catalytic region strongly affect activity.
(14) The oligoribonucleotides were used as substrates in the study of the mechanism of cleavage of an RNA hammerhead domain having the phosphorothioate group at the cleavage site.
(15) These catalytic RNAs, or ribozymes, form a stem-loop secondary structure called a 'hammerhead' in which the catalytic (ribozyme) and substrate sequences are brought close together.
(16) Two sequence variants contained nucleotide changes in the double hammerhead-like self-cleaving structure identified in ASBV RNA.
(17) We have designed a hammerhead-type RNA system which consists of three RNA fragments for normal and modified complexes which contain a non-cleavable substrate with 2'-O-methylcytidine and a guanosine-to-inosine replaced enzyme.
(18) Although related to the hammerhead structure, sequences flanking the plus strand termini showed differences from the consensus and may be folded into a different structure containing a pseudo-knot.
(19) We have constructed and characterised in vitro a number of hammerhead ribozymes designed to cleave individual RNAs encoded by these genes.
(20) Substitutions of DNA for RNA in the various stems of a hammerhead ribozyme have been analyzed in vitro for kinetic efficiency.
Plate
Definition:
(n.) A flat, or nearly flat, piece of metal, the thickness of which is small in comparison with the other dimensions; a thick sheet of metal; as, a steel plate.
(n.) Metallic armor composed of broad pieces.
(n.) Domestic vessels and utensils, as flagons, dishes, cups, etc., wrought in gold or silver.
(n.) Metallic ware which is plated, in distinction from that which is genuine silver or gold.
(n.) A small, shallow, and usually circular, vessel of metal or wood, or of earth glazed and baked, from which food is eaten at table.
(n.) A piece of money, usually silver money.
(n.) A piece of metal on which anything is engraved for the purpose of being printed; hence, an impression from the engraved metal; as, a book illustrated with plates; a fashion plate.
(n.) A page of stereotype, electrotype, or the like, for printing from; as, publisher's plates.
(n.) That part of an artificial set of teeth which fits to the mouth, and holds the teeth in place. It may be of gold, platinum, silver, rubber, celluloid, etc.
(n.) A horizontal timber laid upon a wall, or upon corbels projecting from a wall, and supporting the ends of other timbers; also used specifically of the roof plate which supports the ends of the roof trusses or, in simple work, the feet of the rafters.
(n.) A roundel of silver or tinctured argent.
(n.) A sheet of glass, porcelain, metal, etc., with a coating that is sensitive to light.
(n.) A prize giving to the winner in a contest.
(v. t.) To cover or overlay with gold, silver, or other metals, either by a mechanical process, as hammering, or by a chemical process, as electrotyping.
(v. t.) To cover or overlay with plates of metal; to arm with metal for defense.
(v. t.) To adorn with plated metal; as, a plated harness.
(v. t.) To beat into thin, flat pieces, or laminae.
(v. t.) To calender; as, to plate paper.
Example Sentences:
(1) Epidermal growth factor reduced plating efficiency by about 50% for A431 cells in different cell cycle phases whereas a slight increase in plating efficiency was seen for SiHa cells.
(2) We have measured the antibody specificities to the two polysaccharides in sera from asymptomatic group C meningococcal carriers and vaccinated adults by a new enzyme-linked immunosorbent assay (ELISA) procedure using methylated human serum albumin for coating the group C polysaccharide onto microtiter plates.
(3) The decline in the frequency of serious complications was primarily due to a decrease in the proportion of patients with open fractures treated with plate osteosynthesis from nearly 50% to 19%.
(4) A substance with a chromatographic mobility of Rf = 0.8 on TLC plates having an intact phosphorylcholine head group was also formed but has not yet been identified.
(5) ACh released from the vesicular fraction was about 100-fold more than could be accounted for by miniature end-plate potentials; possible causes of this overestimate are discussed.
(6) It was found to be convenient for routine laboratory use and increased the yield of positive plate cultures in specimens without antibiotics from 53 to 75% (P less than 0.01) and in specimens containing antibiotics from 24 to 38% (P less than 0.05).
(7) For routine use, 50 mul of 12% BTV SRBC, 0.1 ml of a spleen cell suspension, and 0.5 ml of 0.5% agarose in a balanced salt solution were mixed and plated on a microscope slide precoated with 0.1% aqueous agarose.
(8) Dose distributions were evaluated under thin sheet lead used as surface bolus for 4- and 10-MV photons and 6- and 9-MeV electrons using a parallel-plate ion chamber and film.
(9) The analgesic activity of morphine was assessed by the hot-plate technique in the offspring of female CFE rats that had received morphine twice daily on days 5 to 12 of pregnancy.
(10) Using as little as 0.2 ml of human blood per culture plate, we successfully cloned hybridomas and established a hybrid cell line producing anti-peroxidase antibody.
(11) There is approximately a 25% decrease in aggregation from regions of the rib distal to the metaphyseal-growth plate junction (69%) to the region proximal to it (50%).
(12) A total of 63 patients (95%) showed varying degrees of hyperostosis involving the cribiform plate, planum sphenoidale, or tuberculum sellae (including the chiasmatic sulcus).
(13) In the absence of prostigmine, increasing the concentration of ACh in the synaptic cleft did not change the time constant for decay of end-plate currents.
(14) To selectively stain polyanionic macromolecules of growth plate cartilage and to prevent artifacts induced by aqueous fixation, proximal tibial growth plates were excised from rats, slam-frozen, and freeze-substituted in 100% methanol containing the cationic dye Alcian blue.
(15) Measurements of acetylcholine-induced single-channel conductance and null potentials at the amphibian motor end-plate in solutions containing Na, K, Li and Cs ions (Gage & Van Helden, 1979; J. Physiol.
(16) In this study, a technique is described by which large obturators can be retained with an acrylic resin head plate.
(17) After short-term (1 h) incubation in suspension cultures cells were washed and plated in clonogenic agar cultures.
(18) A significant increase in the number of C. albicans CFU in homogenized and plated segments of the GI tract was recognized in mice with murine AIDS versus the control animals.
(19) Silufol plates can be used for the control of the production of vitamins, their analysis in varying biological objects, as well as in biochemistry, medicine and pharmaceutics.
(20) The relative importance of these properties depends critically on the presence and mode of motion of the tectorial plate.