What's the difference between hematein and stain?

Hematein


Definition:

  • (n.) A reddish brown or violet crystalline substance, C16H12O6, got from hematoxylin by partial oxidation, and regarded as analogous to the phthaleins.

Example Sentences:

  • (1) The autoxidation of hematoxylin to hematein was accompanied by an increase in absorbance between 400 and 670 nm.
  • (2) All additives tested (ethyl alcohol, glycerine, chloral hydrate, ethylene and propylene glycol, and citric, malonic and maleic acids) in varying degrees limited the conversion of hematein to insoluble compounds.
  • (3) Brazilein is much more acid-sensitive than hematein.
  • (4) It was shown that of the dye components of Papanicolaou stains only aluminum hematein, orange G, light green SF and eosin Y are bound to cervical cells and that their chromophores do not interact.
  • (5) Furthermore, keratinization was studied with the rhodamine B method and lipids with the oil red O, the OTAN and the acid hematein methods.
  • (6) Hematein and Fe+++ form a variety of cationic, anionic and non-ionic chelates; the ratio of these compounds changes with time.
  • (7) Current chemical concepts were applied to Weigert's, M. Heidenhain's and Verhoeff's iron hemateins, Mayer's acid hemalum stain and the corresponding brazilein compounds.
  • (8) Verhoeff (1908) recommended an iron-hematein formula containing Lugol's solution for demonstration of elastic tissue; sections are differentiated until desired staining patterns are obtained.
  • (9) Since pyridine-extractable acid-fastness (and acid hematein-positivity) serve to distinguish human leprosy bacilli from M. lepraemurium, one or the other, or both, are suggested as bases for differentiating these two organisms in animal experiments designed to show the in vivo propagation of human leprosy bacilli.
  • (10) After pyridine extraction, bromination, or aqueous saponification, true mycobacteria lost neither their acid hematein-positivity nor their acid-fastness.
  • (11) The traditional quinonoid formula of hematein and brazilein was revised.
  • (12) When combined in specified proportions the stock solutions yield aluminum-hematein dissolved in nontoxic propylene glycol.
  • (13) While permanent loss of acid-fastness from leprosy bacilli always resulted in a loss of acid hematein-fixing material (Smith-Dietrich-Baker tests), the reverse was not true.
  • (14) Olation of such chelates affects the staining properties of iron hematein solutions.
  • (15) The acid hematein-positive material and the acid-fastness of both leprosy bacilli and mycobacteria were lost after treatment with alkaline ethanol.
  • (16) On day 4 the animals were sacrificed, the mean tumour diameter measured, the tumour bearing kidney fixed in Bouin's picroformol solution and processed for histological analysis after staining with hematein.
  • (17) The absorption spectra of hematein-aluminium solutions have been recorded at various concentrations and pH values; the solutions were prepared using analytically pure hematein and potassium alum as aluminium source.
  • (18) In aqueous solution, four different hematein-aluminium complexes could be distinguished by absorption spectroscopy.
  • (19) Hematoxylin, a natural dye commonly used as a histological stain, generates superoxide upon oxidation to its quinonoid product, hematein.
  • (20) The topographical and quantitative study was done on frozen 20-mu sections of tissue stained with Sudan IV in propylene glycol and counterstained with hematein (Geigy).

Stain


Definition:

  • (v. t.) To discolor by the application of foreign matter; to make foul; to spot; as, to stain the hand with dye; armor stained with blood.
  • (v. t.) To color, as wood, glass, paper, cloth, or the like, by processess affecting, chemically or otherwise, the material itself; to tinge with a color or colors combining with, or penetrating, the substance; to dye; as, to stain wood with acids, colored washes, paint rubbed in, etc.; to stain glass.
  • (v. t.) To spot with guilt or infamy; to bring reproach on; to blot; to soil; to tarnish.
  • (v. t.) To cause to seem inferior or soiled by comparison.
  • (v. i.) To give or receive a stain; to grow dim.
  • (n.) A discoloration by foreign matter; a spot; as, a stain on a garment or cloth.
  • (n.) A natural spot of a color different from the gound.
  • (n.) Taint of guilt; tarnish; disgrace; reproach.
  • (n.) Cause of reproach; shame.
  • (n.) A tincture; a tinge.

Example Sentences:

  • (1) The patterns observed were: clusters of granules related to the cell membrane; positive staining localized to portions of the cell membrane, and, less commonly, the whole cell circumference.
  • (2) Within the outflow tract wall, the labelled cells were enmeshed by strands of alcian blue-stained extracellular matrix.
  • (3) The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate.
  • (4) The content of the cavities was not stained by any of the immunocytochemical reactions applied.
  • (5) The dependence of fluorescence polarization of stained nerve fibres on the angle between the fibre axis and electrical vector of exciting light (azimuth characteristics) has been considered.
  • (6) Moreover, in DCVC-treated cells the mitochondria could not be stained with rhodamine-123, indicating severe mitochondrial damage and loss of membrane potential.
  • (7) Immunofluorescent staining for HLA-DR showed dermal positivity in 12 of 13 involved- and 9 of 13 uninvolved-skin biopsy specimens from scleroderma patients, compared with only 1 of 10 controls.
  • (8) From these results it was concluded that FITC-Con A staining method applied to smear specimens is more advantageous in the rapidity and the simplicity for tumor cell diagnosis than section specimen method.
  • (9) Further purification of ZAB by filtration through Sephadex G-100 gave a preparation (ZAB2) which contained the common antigen as shown by the cross-reactivity of anti-ZAB2 rat serum with seven stains of N. gonorrhoeae.
  • (10) It has been found that the epidermal staining pattern for ICAM-1 in each of these diseases in distinctive and different in each disease.
  • (11) After either 5 or 10 days of culture with both cytokines, intense immunofluorescent staining for Ia could be identified on the surface of greater than 80-90% of the viable islet cells.
  • (12) In the second comparison, HSV was isolated from 225 of 1,026 (21.9%) specimens and duplicate human foreskin fibroblast cell wells stained at 24 and 72 h were PAP positive in 241 of 1,026 (23.5%).
  • (13) The epithelium of Brunner's gland stained intensely with Ricinus communis agglutinin-I (RCA-I), succinylated-WGA (S-WGA) and wheat-germ agglutinin (WGA), moderately with Bandeirea simplicifolia agglutinin-I (BS-I), Concanavalia ensiformis agglutinin (Con A) peanut agglutinin (PNA) and Ulex europaeus agglutinin-I (UEA-I) and occasionally with Dolichos biflorus agglutinin (DBA), Lens culinaris agglutinin (LCA) and soybean agglutinin (SBA).
  • (14) Using serial section electron microscopic reconstructions as a reference, we have chosen as our standard procedure a method that maximizes both the preservation of the cytoskeleton and the proportion of cells staining, while minimizing the degree of nonspecific staining.
  • (15) One major band with a molecular weight of 12,000 was detected by autofluorography and coincided with the Coomassie staining band of apocytochrome c from S. cerevisiae.
  • (16) Pitlike surface structures seen in negatively stained whole cells and thin sections were correlated with periodically spaced perforations of the rigid sacculus.
  • (17) In the present study, 125 oesophageal biopsies obtained under direct vision at endoscopy from 22 patients with Barrett's oesophagus were systematically studied using fluorescence and peroxidase antiperoxidase single and double-staining immunocytochemical methods employing highly specific antibodies to localize the following peptide-containing cell types in Barrett's mucosa: gastrin, somatostatin, gastric inhibitory polypeptide, motilin, neurotensin and pancreatic glucagon.
  • (18) The rate of nuclei stained by Pr-122 is different from that of Pr-192 in both growing and quiescent cultures.
  • (19) This light microscopic comparison of viable FDA- and nonviable PI-stained cysts of G. muris demonstrates that 2 types of cysts can be distinguished and implies that structural differences can be used to identify these subpopulations of cysts.
  • (20) Benign and malignant epithelial and soft tissue tumors of the skin were usually negatively stained with MoAb HMSA-2.

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