(1) Neurons from rat fetal cerebral hemispheres were grown in a synthetic medium (Maat medium), as previously described, for different periods of time.
(2) Formation of the external aldimine with 2-methylaspartate is accompanied by tilting of the coenzyme ring by 44 degrees in cAAT and 39 degrees in mAAT.
(3) Distributions of the activities of the cytosolic (cAAT) and mitochondrial (mAAT) isoenzymes of aspartate aminotransferase were determined in rat retinal layers.
(4) The distribution of total AAT activity (tAAT = cAAT + mAAT) and of mAAT activity correlated well (r = 0.88-0.91) with the distribution of MDH activity.
(5) These results suggest that testosterone stimulated mAAT activity by induction of pmAAT mRNA.
(6) Phentolamine did not alter the noradrenaline effect on either mAAT or cAAT; it decreased significantly the free form of the mAAT activity only.
(7) This continues to support our proposal that a major physiological effect of testosterone is increased pmAAT mRNA steady-state levels which result in increased pmAAT synthesis and increased mAAT activity.
(8) The pmAAT mRNA induction occurred 30 min after testosterone treatment and was maximal by 1.5 h. Prostatic mAAT activity was also induced by testosterone with a 1-2 h lag period.
(9) Distributions of activity of the cytosolic (cAAT) and mitochondrial (mAAT) isoenzymes of aspartate aminotransferase and of malate dehydrogenase (MDH) were determined in guinea pig retinal layers.
(10) This continues to support our proposal that testosterone regulates prostate citrate production via a stimulatory effect on mAAT which results in increased mitochondrial synthesis of citrate from aspartate.
(11) For the quinonoid complex with erythro-3-hydroxyaspartate, the tilt angles were found to be 63 degrees in cAAT and 53 degrees in mAAT.
(12) The effects of testosterone on mitochondrial aspartate aminotransferase (mAAT) synthesis in rat ventral prostate was investigated.
(13) cAAT was highest in the photoreceptor inner segments, inner nuclear layer and inner plexiform layer; mAAT was highest in the inner segments.
(14) cAAT activity was also highest in the inner segments, but the difference between the activity in the inner segments and the other layers was not nearly as great as with mAAT.
(15) Cytosolic and mitochondrial pig aspartate aminotransferases (cAAT and mAAT) and chicken cAAT were oriented in a compressed slab of polyacrylamide gel.
(16) Maat medium improved the purity and longevity of neuronal cultures.
(17) As a result of protonation, the coenzyme tilts by 27 degrees in cAAT and 13 degrees in mAAT.
(18) Insertion of an 18-nucleotide-long poly(G) tract into the 3'-terminal untranslated region of yeast phosphoglycerate kinase (PGK1) mRNA increases its chemical half-life by about a factor of 2 (P. Vreken, R. Van der Veen, V. C. H. F. de Regt, A. L. de Maat, R. J. Planta, and H. A. Raué, Biochimie 73:729-737, 1991).
(19) Prolactin (PRL) has been reported to stimulate citrate production and the activity of mitochondrial aspartate aminotransferase (mAAT) and its precursor form pmAAT in prostate epithelial cells.
(20) The differences in the coenzyme tilt angles between cAAT and mAAT might be linked to catalytic peculiarities of the isoenzymes.
Mast
Definition:
(n.) The fruit of the oak and beech, or other forest trees; nuts; acorns.
(n.) A pole, or long, strong, round piece of timber, or spar, set upright in a boat or vessel, to sustain the sails, yards, rigging, etc. A mast may also consist of several pieces of timber united by iron bands, or of a hollow pillar of iron or steel.
(n.) The vertical post of a derrick or crane.
(v. t.) To furnish with a mast or masts; to put the masts of in position; as, to mast a ship.
Example Sentences:
(1) of PLA2 caused marked degranulation of mast cells in the rat mesentery which was facilitated by addition of calcium ion (10 mM) but antagonized by pretreating with three antiinflammatory agents.
(2) In later phases, mast cells appeared in the newly formed marrow in the external callus.
(3) Our prospective study has defined a number of important variables in patients with clinical evidence of mast cell proliferation that can predict both the presence of SMCD and the likelihood of fatal disease.
(4) In the dark cortical zone of the nodes (III group) there occur tissue basophils (mast cells), that, together with increasing number of acidophilic granulocytes and appearance of neutrophilic cells, demonstrates that there is an inflammatory reaction in the organ studied as a response to the lymphocytic suspension injected.
(5) Type I and Type II mast-cell degranulation was noted but was not universal.
(6) They clearly demonstrate the phenomenon of mast cells degranulation.
(7) The early absolute but transient dependence of these A-MuLV mast cell transformants on a fibroblast feeder suggests a multistep process in their evolution, in which the acquisition of autonomy from factors of mesenchymal cell origin may play an important role.
(8) The findings suggest that mast cell prostaglandins are an important factor in the pathogenesis of pruritus and that local vascular responses may trigger mast cell degranulation.
(9) 18 patients with typical sporadic Amyotrophic Lateral Sclerosis (ALS) were investigated by the Motor Accuracy and Speed Test (MAST) and 18 healthy age- and-sex-matched volunteers, acted as controls.
(10) When PMC purified to greater than 99% purity were cultured in methylcellulose with IL-3 and IL-4, approximately 25% of the PMC formed colonies, all of which contained both berberine sulfate-positive and berberine sulfate-negative mast cells.
(11) But we sent out reconnoitres in the morning; we send out a team in advance and they get halfway down the road, maybe a quarter of the way down the road, sometimes three-quarters of the way down the road – we tried this three days in a row – and then the shelling starts and while I can’t point the finger at who starts the shelling, we get the absolute assurances from the Ukraine government that it’s not them.” Flags on all Australian government buildings will be flown at half-mast on Thursday, and an interdenominational memorial service will be held at St Patrick’s cathedral in Melbourne from 10.30am.
(12) Mice homozygous for mutations at either locus exhibit several phenotypic abnormalities including a virtual absence of mast cells.
(13) This initial observation of release of eosinophil chemotactic factor of anaphylaxis in vivo along with histamine assigns the mast cell a central role in cold urticaria.
(14) Their presence was established both by staining for mast cells at light microscopic level and by electron microscopy.
(15) Pretreatment of rat peritoneal mast cells with either Staurosporine or an analog K-252a, lead to a dose-related inhibition of histamine release when stimulated with Anti-IgE (IC50: Staurosporine = 110 nM; K-252a = 100 nM).
(16) The ammoniacal silver method, which identifies basic proteins, gives a positive reaction in cytoplasmic granules of rat peritoneal mast cells.
(17) Cytokine secretion by activated lymphocytes or mast cells is preceded by dramatic stabilization of the normally labile GM-CSF mRNA.
(18) Forty-seven patients were brought to the Emergency Department with a good blood pressure which probably would not have existed without the use of MAST Trousers.
(19) Furthermore, using rat mast cells, the binding assay in conjunction with histamine releasing assay may be utilized to predict the in vivo histamine releasing potential of new LHRH peptides which are of clinical importance.
(20) Six dogs had increased numbers of mast cells in peripheral blood or buffy coat smears.