(1) Because many humans have high levels of circulating antibodies directed against the enzymatic product of alpha 1----3-galactosyltransferase (Gal alpha 1----3Gal beta 1----4GlcN Ac) (Galili, U., Clark, M. R., Shohet, S. B., Buehler, J., and Macher, B.
(2) 162, 573-582; Galili, U., Buehler, J., Shohet, S. B., and Macher, B.
(3) This natural antibody, designated "anti-Gal," was previously found to bind to terminal Gal alpha 1----3Gal beta 1----4GlcNAc-R on biochemically defined glycolipids (Galili, U., Macher, B.
(4) Previous studies (Galili, U., Clark, M. R., Shohet, S. B., Buehler, J., and Macher, B.
(5) 84, 1369-1373; Galili, U., Shohet, S. B., Korbrin, E., Stults, C. L. M., and Macher, B.
(6) A mouse monoclonal antibody, VIM-2, specific for human blood cells of myelomonocytic lineage, was found to bind to a series of minor gangliosides isolated from the cells of patients with chronic myelogenous leukemia (Uemura, K., Macher, B.A., DeGregorio, M., Scudder, P., Buehler, J., Knapp, W., and Feizi, T. (1985) Biochim.
(7) alpha 1----3-Galactosyltransferase is widely expressed in different mammalian species, with the notable exception of man and Old World monkeys (Galili, U., Shohet, S. B., Kobrin, E., Stults, C.L.M., and Macher, B.
(8) Thus, in an apparently pure batch of crystalline lipid X as obtained by a published procedure (Macher, I.
Micher
Definition:
(n.) One who skulks, or keeps out of sight; hence, a truant; an idler; a thief, etc.