(1) One hour after direct mechanical cardiomassage (DMCM) a moderately pronounced edema of the intercellular spaces in the basal compartment of the seminiferous epithelium, normal content of lactate and succinate dehydrogenases, and a certain decrease in the activity of glucose-6-phosphate dehydrogenases and NAD- and NADP-diaphorases were noted.
(2) Transformed mammalian cells express both the usual NADP-dependent trifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase-synthetase as well as the bifunctional NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase.
(3) The results are discussed in terms of a two-site model in which separate, but interacting, regions exist on the enzyme to accommodate the adenosine and nicotinamide moieties of NAD, and a single-site model in which the adenosine part of the molecule is bound preferentially and this interacts with the nicotinamide fraction.
(4) The NAD-dependent enzymes (except alpha-GPDH) showed a stronger reactivity in the proximal tubules, while the NADP-dependent ones were more reactive in the thick limb of Henle's loop and distal convoluted tubules.
(5) In contrast, AMP and adenosine did not alter NAD glycohydrolase activity or affect subunit structure.
(6) With cortisol and cortisol-21-aldehyde, product inhibition patterns showed only slope effects with steroid product and NAD+, suggesting a "random" mechanism.
(7) Kinetic studies of glutamate dehydrogenase were made with wide concentration ranges of the coenzymes NAD(+) and NADP(+) and the substrates glutamate and norvaline.
(8) Both substances decreased the concentrations of NAD(+) in the islet cells to about 10% of the control values within 2h after injection.
(9) We investigated the denaturation of tetrameric 20 beta-hydroxysteroid dehydrogenase (20R)-17 beta,20 beta,21-trihydroxysteroid:NAD+ oxidoreductase, EC 1.1.1.53) to find out whether intermediate states are formed during the process.
(10) On subfractionation of this crude mitochondrial fraction with continuous sucrose density gradients, most of the activity of the three enzymes was found at a higher density than NAD+-isocitrate dehydrogenase and at about the same density as glutamate dehydrogenase, confirming earlier reported data for acetyl-CoA synthase.
(11) The effect of dicoumarol on glucuronidation of 3-OH-benzo(a)pyrene (BP) appears to be due to inhibition of UDPglucuronosyltransferase (UDPGT) and not to an inhibited DT-diaphorase (NAD(P)H:quinone oxidoreductase); to date the only enzyme known to be inhibited by dicoumarol.
(12) The increase in concentration of organic cosolvents results in a 2-2.5-fold increase of the maximal reaction rate and a decrease of Michaelis constant for formate of NAD(+)-dependent formate dehydrogenase from methylotrophic bacteria Pseudomonas sp.
(13) Furthermore, NADP was over 1000-fold more potent than NAD in inhibiting the enzyme competitively with respect to NADH.
(14) The possible participation of a Ca(2+)-induced activation of NAD-isocitrate dehydrogenase in this process was investigated.
(15) A 4.1-kb EcoRI fragment which includes the gene (gldA) encoding a glycerol dehydrogenase (G1DH; EC 1.1.1.6; glycerol:NAD oxidoreductase) from Bacillus stearothermophilus var.
(16) It is supposed that the stimulating effect of lactate with NAD+ on the mitochondria respiration is not so much a result of the membrane-damaged action as a result of oxidation of lactate dehydrogenase reaction products: phosphorylative oxidation of pyruvate and nonconjugated oxidation of NADH.
(17) In the presence of glycolate (glyoxylate), and NADH and NAD alone or together in physiological proportions, the rate of serine-to-glycerate conversion was enhanced and sustained by the addition of malate.
(18) Virazole 5'-phosphate was subsequently found to be a potent competitive inhibitor of inosine 5'-phosphate dehydrogenase (IMP:NAD(+) oxidoreductase, EC 1.2.1.14) isolated from Escherichia coli (K(m) = 1.8 x 10(-5) M) with a K(i) of 2.7 x 10(-7) M. Guanosine 5'-phosphate (GMP) was a competitive inhibitor of this enzyme with a K(i) of 7.7 x 10(-5) M. Virazole 5'-phosphate was similarly active against IMP dehydrogenase isolated from Ehrlich ascites tumor cells, with a K(i) of 2.5 x 10(-7) M. The K(m) for this enzyme was 1.8 x 10(-5) M, and the K(i) for GMP was 2.2 x 10(-4) M. These results suggest that the antiviral activity of Virazole might be due to the inhibition of GMP biosynthesis in the infected cell at the step involving the conversion of IMP to xanthosine 5'-phosphate.
(19) The methods described make possible the preparation of fossil samples for light nad transmission electron microscopy.
(20) The activity of soluble NAD-specific hydrogenase was not detected.
Nag
Definition:
(n.) A small horse; a pony; hence, any horse.
(n.) A paramour; -- in contempt.
(v. t. & i.) To tease in a petty way; to scold habitually; to annoy; to fret pertinaciously.
Example Sentences:
(1) There were no significant differences in the NAG indices either between fullterm and preterm babies or between appropriate for gestational age (AGA) and small for gestational age (SGA) neonates of the normal group.
(2) Urinary excretion of the tubular enzymes NAG and AAP was investigated during gentamicin treatment of 105 newborn infants.
(3) Elevated urinary levels of the renal tubular enzyme N-acetyl-beta-D-glucosaminidase (NAG) have been shown to be associated with tubular damage.
(4) Urine N-acetyl-beta-glucosaminidase (NAG) was measured 3 times weekly and serum creatinine was measured 5 times during the study.
(5) The nag genes appear to be organized in an operon: nagD nagC nagA nagB nagE.
(6) About 10% of the exposed workers had an enhanced NAG value, corresponding to the level of diabetic patients with subclinical nephropathy.
(7) The aim of the present study was to evaluate the trend of excretion of the lysosomal enzyme N-acetyl-beta-D-glucosaminidase (NAG) and the brush border enzyme alanine aminopeptidase (AAP) during uncomplicated pregnancies.
(8) A prospective study in 16 healthy and 16 gentamicin-treated neonates was undertaken to compare the urinary excretion of proximal tubular markers such as beta 2-microglobulin (beta 2-m) and total N-acetyl-beta-D-glucosaminidase (NAG) and its isoenzymatic form NAGB.
(9) Although NaY and NaG are effective heavy metal chelators and renal function is spared and kidney platinum levels are substantially reduced by the dithiocarbamates, no parallel loss of antineoplastic activity by Cis-Pt on the rat Walker carcinoma was observed.
(10) Epididymal spermatozoa also contained moderate beta-NAG activity.
(11) ), Botryodiplodia theobromae, Pat., Rhizopus arrhizus Fischer., Phomopsis psidii Nag Raj and Ponnappa apud Ponnappa and Nag Raj, and Pestalotiopsis versicolor (Speg.)
(12) In the song Christmas and Owen argue that if women were a Pot Noodle it would be "farewell to nagging and random tantrums".
(13) Urinary retinol binding protein, myoglobin, and N-acetyl-beta-D-glucosaminidase (NAG), expressed as a ratio with urinary creatinine, were measured and excretion profiles repeated at 3-6 days in 15 infants and at 7-14 days in 11 infants.
(14) My nagging suspicion is that the White House is very happy to have a vacuum in the under-secretary and assistant secretary levels, not only at state but across government agencies, because it relieves them of even feeling an obligation to consult with experts before they take a new direction.” In normal times, the state department is a constant part of an inter-agency policymaking process coordinated by the national security council.
(15) A synthetic oligonucleotide probe was developed to identify the gene for the heat-stable enterotoxin (NAG-ST) of non-serovar O1 Vibrio cholerae.
(16) The pH optima of NAG I-IV ranged from 4.2 to 4.4, and Km values from 0.61 to 0.83 mM.
(17) Removal of the endothelium, treatment of endothelium-intact preparations with L-NAG or L-NMMA, or exposure of these vessels to the guanylate cyclase inhibitor, methylene blue (10 microM) increased reactivity of the aortas to the guanosine 3':5'-cyclic monophosphate (cGMP) analogue, 8-Br cGMP.
(18) No correlation was found between activity of beta-NAG and of GPT, gammaGT and CHE.
(19) Although DDTC or NaG pretreatment is highly effective when used in conjunction with NaG post-treatment, DDTC or NaG pretreatment alone has no renal sparing effect on renal function or renal platinum accumulation.
(20) Supplemental fosfomycin or steroids inhibited an increase in urinary NAG level 3 days after the anticancer therapy.