(1) One hour after direct mechanical cardiomassage (DMCM) a moderately pronounced edema of the intercellular spaces in the basal compartment of the seminiferous epithelium, normal content of lactate and succinate dehydrogenases, and a certain decrease in the activity of glucose-6-phosphate dehydrogenases and NAD- and NADP-diaphorases were noted.
(2) Transformed mammalian cells express both the usual NADP-dependent trifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase-synthetase as well as the bifunctional NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase.
(3) The results are discussed in terms of a two-site model in which separate, but interacting, regions exist on the enzyme to accommodate the adenosine and nicotinamide moieties of NAD, and a single-site model in which the adenosine part of the molecule is bound preferentially and this interacts with the nicotinamide fraction.
(4) The NAD-dependent enzymes (except alpha-GPDH) showed a stronger reactivity in the proximal tubules, while the NADP-dependent ones were more reactive in the thick limb of Henle's loop and distal convoluted tubules.
(5) In contrast, AMP and adenosine did not alter NAD glycohydrolase activity or affect subunit structure.
(6) With cortisol and cortisol-21-aldehyde, product inhibition patterns showed only slope effects with steroid product and NAD+, suggesting a "random" mechanism.
(7) Kinetic studies of glutamate dehydrogenase were made with wide concentration ranges of the coenzymes NAD(+) and NADP(+) and the substrates glutamate and norvaline.
(8) Both substances decreased the concentrations of NAD(+) in the islet cells to about 10% of the control values within 2h after injection.
(9) We investigated the denaturation of tetrameric 20 beta-hydroxysteroid dehydrogenase (20R)-17 beta,20 beta,21-trihydroxysteroid:NAD+ oxidoreductase, EC 1.1.1.53) to find out whether intermediate states are formed during the process.
(10) On subfractionation of this crude mitochondrial fraction with continuous sucrose density gradients, most of the activity of the three enzymes was found at a higher density than NAD+-isocitrate dehydrogenase and at about the same density as glutamate dehydrogenase, confirming earlier reported data for acetyl-CoA synthase.
(11) The effect of dicoumarol on glucuronidation of 3-OH-benzo(a)pyrene (BP) appears to be due to inhibition of UDPglucuronosyltransferase (UDPGT) and not to an inhibited DT-diaphorase (NAD(P)H:quinone oxidoreductase); to date the only enzyme known to be inhibited by dicoumarol.
(12) The increase in concentration of organic cosolvents results in a 2-2.5-fold increase of the maximal reaction rate and a decrease of Michaelis constant for formate of NAD(+)-dependent formate dehydrogenase from methylotrophic bacteria Pseudomonas sp.
(13) Furthermore, NADP was over 1000-fold more potent than NAD in inhibiting the enzyme competitively with respect to NADH.
(14) The possible participation of a Ca(2+)-induced activation of NAD-isocitrate dehydrogenase in this process was investigated.
(15) A 4.1-kb EcoRI fragment which includes the gene (gldA) encoding a glycerol dehydrogenase (G1DH; EC 1.1.1.6; glycerol:NAD oxidoreductase) from Bacillus stearothermophilus var.
(16) It is supposed that the stimulating effect of lactate with NAD+ on the mitochondria respiration is not so much a result of the membrane-damaged action as a result of oxidation of lactate dehydrogenase reaction products: phosphorylative oxidation of pyruvate and nonconjugated oxidation of NADH.
(17) In the presence of glycolate (glyoxylate), and NADH and NAD alone or together in physiological proportions, the rate of serine-to-glycerate conversion was enhanced and sustained by the addition of malate.
(18) Virazole 5'-phosphate was subsequently found to be a potent competitive inhibitor of inosine 5'-phosphate dehydrogenase (IMP:NAD(+) oxidoreductase, EC 1.2.1.14) isolated from Escherichia coli (K(m) = 1.8 x 10(-5) M) with a K(i) of 2.7 x 10(-7) M. Guanosine 5'-phosphate (GMP) was a competitive inhibitor of this enzyme with a K(i) of 7.7 x 10(-5) M. Virazole 5'-phosphate was similarly active against IMP dehydrogenase isolated from Ehrlich ascites tumor cells, with a K(i) of 2.5 x 10(-7) M. The K(m) for this enzyme was 1.8 x 10(-5) M, and the K(i) for GMP was 2.2 x 10(-4) M. These results suggest that the antiviral activity of Virazole might be due to the inhibition of GMP biosynthesis in the infected cell at the step involving the conversion of IMP to xanthosine 5'-phosphate.
(19) The methods described make possible the preparation of fossil samples for light nad transmission electron microscopy.
(20) The activity of soluble NAD-specific hydrogenase was not detected.
Nard
Definition:
(n.) An East Indian plant (Nardostachys Jatamansi) of the Valerian family, used from remote ages in Oriental perfumery.
(n.) An ointment prepared partly from this plant. See Spikenard.
(n.) A kind of grass (Nardus stricta) of little value, found in Europe and Asia.
Example Sentences:
(1) They sum up the various methods of prevention of venous stasis: Nard's method, associating bandages and deambulation, as well as various techniques of contention, hemodilution, compression with inflatable boots, electric stimulation or assisted mobilization.
(2) The signal perceived by the NARD appears to have been a valuable warning, rightly casting doubt on the safety of triazolam and the original dosage recommendations.
(3) In the course of 1979 the Netherlands Centre for Monitoring of Adverse Reactions to Drugs (NARD) received a remarkably large number of reports on patients with unusual and complex psychic disturbances, attributed to the use of the then recently marketed hypnotic triazolam.
(4) In consequence both cases were treated as outpatients by physical compression (Nard's method), without any anticoagulant medication : the results were striking and lasting.
(5) It is proposed that molecular oxygen controls the expression of nar via Fnr and that the nard mutation affects the Fnr binding site of the narGHI control region.
(6) The authors looked back at the original publications, that is to say to the publications of Chalier and of Nard, who described methods, which have been much referred to, that were quite exacting.
(7) The synergic effect of walking is definitively established; the treatment of deep-set phlebites by ambulatory compression is discovered by H. Fischer in Germany and then in France by L. Nard.
(8) The nard mutation, located upstream of the nar structural genes, was found to be cis dominant; it led to independence from the Fnr protein which, in the wild-type strain, exerts a strict positive control on the nar operon.