(n.) A reddish brown amorphous dyestuff, /, obtained from orcin, and forming the essential coloring matter of cudbear and archil. It is closely related to litmus.
Example Sentences:
(1) The orcein staining method seems to be a reliable addition to differentiating histologically between PBC and CAH.
(2) Microscopic examinations of eggs stained with aceto-orcein or the DNA fluorochrome bisbenzimide and direct observations on isolated sperm aster complexes show that halothane induces polyspermy (multiple sperm entry) when present at fertilization.
(3) 5 in whom a clinical and histological diagnosis of Indian Childhood Cirrhosis was made had massive orcein-staining deposits in liver cells.
(4) Determination of fungal elastase, however, requires partial purification of culture extracts and the orcein elastin or gravimetric method.
(6) The orcein positive substance localized in the cytoplasm of hepatocytes, less often it was also seen in a few Kupffer cells.
(7) Aceto-orcein and Giemsa when used cold were found to produce relatively artefact-free preparations.
(8) Orcein-positive material in Kupffer cells was not associated with HBsAg as evaluated by the immune peroxidase method.
(9) This enzyme was inactivated by diisopropyl fluorophosphate (DFP), phenylmethyl sulphonylfluoride (PMSF), soybean trypsin inhibitor (SBTI), or elastatinal, suggesting a seryl protease resembling elastase, but it failed to digest elastin-orcein.
(10) This group of patients may therefore have both biliary disease are hepatocellular damage, and can be separated from CAH by the orcein method.
(11) These HBcAg inclusions stain greyish pink with chromotrope aniline blue and are negative for orcein, the periodic acid-Schiff reaction, and the Feulgen reaction for DNA.
(12) Liver specimens from 103 patients with various hepatic diseases and from 297 consecutive liver biopsies examined routinely were stained with orcein after oxidation of the tissue sections with potassium permanganate.
(13) One conspicuous feature was the substantially reduced quantity of positive elements in comparison with the results of staining with aldehydefuchsine and orceine.
(14) Cytofluorometric measurements of orcein-stained chromatin revealed an emission peak at 585 nm with a shoulder at 620 nm.
(15) Orcein-positive material was very frequently found in protracted viral hepatitis and in chronic active hepatitis, as well as in other liver diseases with or without cholestasis; it was absent in liver cirrhosis.
(16) The pattern of copper distribution in human newborn liver was investigated by histochemical methods (rhodamine, orcein and rubeanic acid) and by atomic absorption spectroscopy.
(17) OPHM in HCC was stained with orcein when the tissue sections were preoxidized.
(18) It did not correspond to the pattern and texture of material stained with PAS, Sudan Black or acid orcein.
(19) root tips) and involves the treatment of root tips with 1-2% solution of trypsin either in buffer or in 0.5 N HCl for 5-10 minutes at 37 C or for 30-60 minutes near 0 C followed by staining with 1.5% acetic orcein: 1 N HCl (19:1).
(20) All 17 biopsy specimens from patients with Wilson's disease had high liver copper concentrations, but only nine had positive staining for copper, and six were orcein positive.
Stain
Definition:
(v. t.) To discolor by the application of foreign matter; to make foul; to spot; as, to stain the hand with dye; armor stained with blood.
(v. t.) To color, as wood, glass, paper, cloth, or the like, by processess affecting, chemically or otherwise, the material itself; to tinge with a color or colors combining with, or penetrating, the substance; to dye; as, to stain wood with acids, colored washes, paint rubbed in, etc.; to stain glass.
(v. t.) To spot with guilt or infamy; to bring reproach on; to blot; to soil; to tarnish.
(v. t.) To cause to seem inferior or soiled by comparison.
(v. i.) To give or receive a stain; to grow dim.
(n.) A discoloration by foreign matter; a spot; as, a stain on a garment or cloth.
(n.) A natural spot of a color different from the gound.
(n.) Taint of guilt; tarnish; disgrace; reproach.
(n.) Cause of reproach; shame.
(n.) A tincture; a tinge.
Example Sentences:
(1) The patterns observed were: clusters of granules related to the cell membrane; positive staining localized to portions of the cell membrane, and, less commonly, the whole cell circumference.
(2) Within the outflow tract wall, the labelled cells were enmeshed by strands of alcian blue-stained extracellular matrix.
(3) The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate.
(4) The content of the cavities was not stained by any of the immunocytochemical reactions applied.
(5) The dependence of fluorescence polarization of stained nerve fibres on the angle between the fibre axis and electrical vector of exciting light (azimuth characteristics) has been considered.
(6) Moreover, in DCVC-treated cells the mitochondria could not be stained with rhodamine-123, indicating severe mitochondrial damage and loss of membrane potential.
(7) Immunofluorescent staining for HLA-DR showed dermal positivity in 12 of 13 involved- and 9 of 13 uninvolved-skin biopsy specimens from scleroderma patients, compared with only 1 of 10 controls.
(8) From these results it was concluded that FITC-Con A staining method applied to smear specimens is more advantageous in the rapidity and the simplicity for tumor cell diagnosis than section specimen method.
(9) Further purification of ZAB by filtration through Sephadex G-100 gave a preparation (ZAB2) which contained the common antigen as shown by the cross-reactivity of anti-ZAB2 rat serum with seven stains of N. gonorrhoeae.
(10) It has been found that the epidermal staining pattern for ICAM-1 in each of these diseases in distinctive and different in each disease.
(11) After either 5 or 10 days of culture with both cytokines, intense immunofluorescent staining for Ia could be identified on the surface of greater than 80-90% of the viable islet cells.
(12) In the second comparison, HSV was isolated from 225 of 1,026 (21.9%) specimens and duplicate human foreskin fibroblast cell wells stained at 24 and 72 h were PAP positive in 241 of 1,026 (23.5%).
(13) The epithelium of Brunner's gland stained intensely with Ricinus communis agglutinin-I (RCA-I), succinylated-WGA (S-WGA) and wheat-germ agglutinin (WGA), moderately with Bandeirea simplicifolia agglutinin-I (BS-I), Concanavalia ensiformis agglutinin (Con A) peanut agglutinin (PNA) and Ulex europaeus agglutinin-I (UEA-I) and occasionally with Dolichos biflorus agglutinin (DBA), Lens culinaris agglutinin (LCA) and soybean agglutinin (SBA).
(14) Using serial section electron microscopic reconstructions as a reference, we have chosen as our standard procedure a method that maximizes both the preservation of the cytoskeleton and the proportion of cells staining, while minimizing the degree of nonspecific staining.
(15) One major band with a molecular weight of 12,000 was detected by autofluorography and coincided with the Coomassie staining band of apocytochrome c from S. cerevisiae.
(16) Pitlike surface structures seen in negatively stained whole cells and thin sections were correlated with periodically spaced perforations of the rigid sacculus.
(17) In the present study, 125 oesophageal biopsies obtained under direct vision at endoscopy from 22 patients with Barrett's oesophagus were systematically studied using fluorescence and peroxidase antiperoxidase single and double-staining immunocytochemical methods employing highly specific antibodies to localize the following peptide-containing cell types in Barrett's mucosa: gastrin, somatostatin, gastric inhibitory polypeptide, motilin, neurotensin and pancreatic glucagon.
(18) The rate of nuclei stained by Pr-122 is different from that of Pr-192 in both growing and quiescent cultures.
(19) This light microscopic comparison of viable FDA- and nonviable PI-stained cysts of G. muris demonstrates that 2 types of cysts can be distinguished and implies that structural differences can be used to identify these subpopulations of cysts.
(20) Benign and malignant epithelial and soft tissue tumors of the skin were usually negatively stained with MoAb HMSA-2.