(1) Comparison of wild type and the mutant parD promoter sequences indicated that three short repeats are likely involved in the negative regulation of this promoter.
(2) In contrast to CcdB, the Kid protein of the ParD system induces RecA-dependent cleavage of the cI repressor of bacteriophage lambda very inefficiently or not at all.
(3) The rate of admissions to psychiatric hospitals of patients with primary alcohol-related diagnoses (PARD) has increased from 1971 to 1983 and they now account for 19.7% of all admissions.
(4) Since the parDE operon requires the presence of the parCBA operon for efficient stabilization under certain growth conditions, the potential role of multimer resolution in plasmid stabilization was tested by substituting the ColE1 cer site for the parCBA operon.
(5) The system, which we have called ParD, maps inside the 1.45-kb PstI-EcoRI fragment that is adjacent to the origin of replication of the plasmid.
(6) And then I read another article recently that I am coming and watch over your shoulder Pards.
(7) Both of these promoters are repressed by their own gene products in the same manner in all three bacteria tested, with ParA functioning as the primary repressor of p-parCBA and ParD functioning as the repressor of p-parD.
(8) The ParD protein appears to bind to one inverted repeat sequence, located between the -35 and -10 boxes of p-parD.
(9) In vitro mutagenesis with hydroxylamine of a ParD- miniderivative of R1, pAB174, yielded mutants that were less stable in the cell than pAB174.
(10) The deletion analysis further allowed a redefinition of the minimal functional region as 790 bp in length, consisting of the parD gene (243 bp) and its promoter as well as sequences downstream of parD.
(11) When I speak with Pards tomorrow he may see some other position that he wants to look better of or get a better player for that area.
(12) Determination of the 5' ends of ParD transcripts, revealed that the genes coding for these proteins are transcribed from a single promoter.
(13) While the cer site did function to resolve plasmid multimers, it was not sufficient to restore stabilization activity to the parDE operon under growth conditions that require the parCBA operon for plasmid stability.
(14) The phenotype of a recently-described mutant (OV6), conditionally defective in chromosome partitioning and septal positioning, was originally thought to be due to a new gene (parD) mapping at 88.4 min.
(15) Transcriptional fusions were used to show that parA, parB, and parC are combined in an operon, while parD constitutes a separate transcription unit.
(16) The stability determined by the systems ParD of plasmid R1 and Ccd of plasmid F is due to the concerted action of two proteins, a cytotoxin and an antagonist of this function.
(17) In this paper we report that CcdA and Kis proteins, the antagonists of the Ccd and ParD systems respectively, share significant sequence homologies at both ends.
(18) The proteins P10 and P12 have been shown to be gene products of a new stability system, ParD, of plasmid R1.
(19) We have now shown that, in addition to the parD mutation, OV6 carries a gyrAam mutation and that this mutation is probably responsible for the gross phenotype of the mutant.
(20) Two divergently arranged promoters located in the intercistronic region between parC and parD mediate transcription of these genes.
Sard
Definition:
(n.) A variety of carnelian, of a rich reddish yellow or brownish red color. See the Note under Chalcedony.
Example Sentences:
(1) It is proposed that all SARDs act on some aspect of the central reaction in the ongoing immune response where antigen presentation to T helper cells results in interleukin 2 production and the generation of activated T cells.
(2) The effects produced by a single agent in vivo and in vitro are not always the same so a single mode of action, for example through possession of a thiol group, cannot explain the effects of all SARDs.
(3) Review of the known activity of SARDs on different cell types at various anatomical sites suggest that in fact different SARD drugs act in differing and sometimes conflicting ways.
(4) Whether these findings explain the low incidence of SARD with cuprammonium cellulose plate dialyzers that do not contain potting material is a matter for continued study and experimentation.
(5) Plasma, bronchoalveolar lavage fluids (BAL), and lung parenchyma were analyzed for vitamin E and polyunsaturated fatty acid (PUFA) concentrations in three groups of patients routinely receiving oxygen therapy--two with adult respiratory distress syndrome (ARDS and SARDS), a third with pneumonia (PNEU), as well as a fourth group of patients receiving little or no oxygen therapy (OTHER).
(6) However, none of the SARDs examined could scavenge O2- at concentrations reported in patients' plasma.
(7) This approach for combination therapy whereby a second SARD is given to patients already established on a single SARD, appears to minimise the toxicity which is a problem when 2 SARDs are started simultaneously.
(8) "These instruments were not invented by Greece, nor did investment banks discover them just for Greece," said Christophoros Sardelis, who was chief of Greece's debt management agency when the contracts were conducted with Goldman Sachs.Such contracts were also used by other European countries until Eurostat, the EU's statistic agency, stopped accepting them later in the decade.
(9) During the first year none of the reactions were serious although 9 of the 29 patients (31%) given D-penicillamine and 3 of the 9 patients receiving aurothiomalate developed side-effects requiring withdrawal of the second SARD.
(10) A gas chromatographic system with wide-bore capillary columns and synchronized accumulating radioisotope detector (SARD) was developed.
(11) The site of action of SARDs within the body--whether at the level of synovial inflammation or of the systemic immune response--is largely undetermined.
(12) Since many drugs, particularly the slow-acting anti-rheumatic drugs (SARDs) used in RA, may alter O2- metabolism, the effects of SARDs on SSA were also studied.
(13) The performance of wide-bore capillary columns was good and the correspondence of the resolution obtained with SARD and that with mass detection was excellent.
(14) This open study examined the safety of adding a second slow-acting anti-rheumatic drug (SARD) - D-penicillamine or sodium aurothiomalate - to the therapy of 38 rheumatoid patients already established on sulphasalazine.
(15) The slow acting anti-rheumatic drugs (SARDS) are a chemically heterogeneous group.
(16) The SSA of the plasma, PB-PMN, JF and JF-PMN were significantly higher in patients treated with SARDs than those without.