(n.) an instrument or device for priming; esp., a cap, tube, or water containing percussion powder or other compound for igniting a charge of gunpowder.
(a.) First; original; primary.
(n.) Originally, a small prayer book for church service, containing the little office of the Virgin Mary; also, a work of elementary religious instruction.
(n.) A small elementary book for teaching children to read; a reading or spelling book for a beginner.
(n.) A kind of type, of which there are two species; one, called long primer, intermediate in size between bourgeois and small pica [see Long primer]; the other, called great primer, larger than pica.
Example Sentences:
(1) The following conclusions emerge: (i) when the 3' or the 3' penultimate base of the oligonucleotide mismatched an allele, no amplification product could be detected; (ii) when the mismatches were 3 and 4 bases from the 3' end of the primer, differential amplification was still observed, but only at certain concentrations of magnesium chloride; (iii) the mismatched allele can be detected in the presence of a 40-fold excess of the matched allele; (iv) primers as short as 13 nucleotides were effective; and (v) the specificity of the amplification could be overwhelmed by greatly increasing the concentration of target DNA.
(2) Elongation of existing RNA primers by the human polymerase-primase was semi-processive; following primer binding the DNA polymerase continuously incorporated 20 to 50 nucleotides, then it dissociated from the template DNA.
(3) This cDNA was obtained because of an identical 10 bp match with the 3' end of one of the GnRH primers.
(4) When PCR products in each of the 12 cats were subjected to a second amplification using the same primer pair (two-step amplification: double PCR), FIV proviral DNA was detected in all of the cats.
(5) In junctions, 3' PSS termini are preserved by fill-in DNA synthesis, although their 5' recessed ends cannot serve as a primer.
(6) Previous studies demonstrated that, when poly(dT).oligo(dA) was used as a template-primer, both proteins were required for poly(dA) synthesis.
(7) 5'-Ends of transcripts of the region were located by S1 nuclease mapping and primer extension.
(8) Nucleotide incorporation kinetics were determined and sequence specific pausing was analyzed by primer-extension.
(9) Primer extension and S1 nuclease protection analysis demonstrate that the hepatic and kidney specific mRNAs are transcriptionally initiated at different sites within the sialyltransferase gene.
(10) alpha 1 and alpha 2 were very similar as DNA polymerases in their sensitivity to several inhibitors and their preference for template-primers, except that alpha 1 had a slightly greater preference for poly (dT) X (rA)10 than alpha 2 did.
(11) Our results indicate that provirus expression occurs by two different mechanisms: one provirus acquired a single base pair mutation in the retrovirus tRNA primer binding site, permitting provirus expression; expression of three proviruses was mediated by 5'-flanking DNA sequences.
(12) Cognate sites in genomes that diverged approximately 100 million years ago can be detected by PCR assays based on primer pairs from unique sequences.
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) This reaction involved the synthesis of a short oligoribonucleotide primer which was then extended into a DNA chain.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) B.subtilis phage M2 uses a protein, instead of RNA, as the primer of its DNA replication.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) Primer extension experiments show that in fission yeast transcripts are initiated at the same starting point as in tomato, indicating for the first time that a plant promoter can be correctly recognized in fission yeast.
(19) Genomic clones for the mouse estrogen receptor have been isolated from a cosmid library and used in conjunction with the cDNA clones to study the expression of the receptor in vivo by RNase mapping, primer extension, and Northern blotting.
(20) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
Priming
Definition:
(p. pr. & vb. n.) of Prime
(n.) The powder or other combustible used to communicate fire to a charge of gunpowder, as in a firearm.
(n.) The first coating of color, size, or the like, laid on canvas, or on a building, or other surface.
(n.) The carrying over of water, with the steam, from the boiler, as into the cylinder.
Example Sentences:
(1) We conclude that the priming effect is not a clinically significant phenomenon during natural pollen exposure in allergic rhinitis patients.
(2) The results indicate that OA-bearing macrophages primed T cells and generated helper T cells, whereas the culture of normal lymphocytes with soluble OA in the absence of macrophages generated suppressor T cells.
(3) PMNs could be primed for PMA-triggered oxidative burst by muramyl peptide molecules (MDP) and two of its adjuvant active nonpyrogenic derivatives.
(4) Brown's model, which goes far further than those from any other senior Labour figure, and the modest new income tax powers for Holyrood devised when he was prime minister, edge the party much closer to the quasi-federal plans championed by the Liberal Democrats.
(5) For related pairs, both the primes (first pictures) and targets (second pictures) varied in rated "typicality" (Rosch, 1975), being either typical or relatively atypical members of their primary superordinate category.
(6) One-nation prime ministers like Cameron found the libertarians useful for voting against taxation; inconvenient when they got too loud about heavy-handed government.
(7) Critics say he is unelectable as prime minister and will never be able to implement his plans, but he has nonetheless pulled attention back to an issue that many thought had gone away for good.
(8) We conclude that both exogenously applied PAF by inhalation and antigen exposure are capable of inducing LAR in sensitized guinea pigs, and thus the priming effect of immunization and PAF may contribute to the development of LAR observed in asthma.
(9) The surge the prime minister talks about can only be achieved by coordinating assets across 43 forces.
(10) As evidence, they show no mediated semantic-phonological priming during picture naming: Retrieval of sheep primes goat, but the activation of goat is not transmitted to its phonological relative, goal.
(11) Among the guests invited to witness the flypast were six second world war RAF pilots, dubbed the “few” by the wartime prime minister, Winston Churchill.
(12) Speaking to a handpicked audience of community representatives, the prime minister said he had not allowed the EU to get its way.
(13) The prime minister’s spokeswoman said: “We think this can be done in line with EU and international law and it is important it is introduced and set up in the right way.
(14) James Cameron, vice-chairman of Climate Change Capital , an environmental investment group, and a member of the prime minister's Business Advisory Group , says: "I think the UK has, in essence, become a better place for green investors.
(15) Although alum adsorbed allergen could induce IgE synthesis in mice primed with liposome entrapped allergen the increase in serum specific IgE levels was lower than the animals primed and challenged with alum adsorbed allergen.
(16) David Cameron was accused of revealing his ill-suppressed Bullingdon Club instincts when he shouted at the Labour frontbencher Angela Eagle to "calm down, dear" as she berated him for misleading MPs at prime minister's questions.
(17) The appointment of the mayor of London's brother, who formally becomes a Cabinet Office minister, is one of a series of moves designed to strengthen the political operation in Downing Street and to patch up the prime minister's frayed links with the Conservative party.
(18) On raw music scores a sex-linked, time-of-day-induced priming effect was due to the prior presentation of CVs--that is, cognitive priming.
(19) The citizenship debate is tawdry, conflated and ultimately pointless | Richard Ackland Read more On Wednesday, the prime minister criticised lawyers for backing terrorists.
(20) The prime minister insisted, however, that he and other world leaders were not being stubborn over demands that the Syrian leader, President Bashar al-Assad, step down at the end of the peace process.