(n.) an instrument or device for priming; esp., a cap, tube, or water containing percussion powder or other compound for igniting a charge of gunpowder.
(a.) First; original; primary.
(n.) Originally, a small prayer book for church service, containing the little office of the Virgin Mary; also, a work of elementary religious instruction.
(n.) A small elementary book for teaching children to read; a reading or spelling book for a beginner.
(n.) A kind of type, of which there are two species; one, called long primer, intermediate in size between bourgeois and small pica [see Long primer]; the other, called great primer, larger than pica.
Example Sentences:
(1) The following conclusions emerge: (i) when the 3' or the 3' penultimate base of the oligonucleotide mismatched an allele, no amplification product could be detected; (ii) when the mismatches were 3 and 4 bases from the 3' end of the primer, differential amplification was still observed, but only at certain concentrations of magnesium chloride; (iii) the mismatched allele can be detected in the presence of a 40-fold excess of the matched allele; (iv) primers as short as 13 nucleotides were effective; and (v) the specificity of the amplification could be overwhelmed by greatly increasing the concentration of target DNA.
(2) Elongation of existing RNA primers by the human polymerase-primase was semi-processive; following primer binding the DNA polymerase continuously incorporated 20 to 50 nucleotides, then it dissociated from the template DNA.
(3) This cDNA was obtained because of an identical 10 bp match with the 3' end of one of the GnRH primers.
(4) When PCR products in each of the 12 cats were subjected to a second amplification using the same primer pair (two-step amplification: double PCR), FIV proviral DNA was detected in all of the cats.
(5) In junctions, 3' PSS termini are preserved by fill-in DNA synthesis, although their 5' recessed ends cannot serve as a primer.
(6) Previous studies demonstrated that, when poly(dT).oligo(dA) was used as a template-primer, both proteins were required for poly(dA) synthesis.
(7) 5'-Ends of transcripts of the region were located by S1 nuclease mapping and primer extension.
(8) Nucleotide incorporation kinetics were determined and sequence specific pausing was analyzed by primer-extension.
(9) Primer extension and S1 nuclease protection analysis demonstrate that the hepatic and kidney specific mRNAs are transcriptionally initiated at different sites within the sialyltransferase gene.
(10) alpha 1 and alpha 2 were very similar as DNA polymerases in their sensitivity to several inhibitors and their preference for template-primers, except that alpha 1 had a slightly greater preference for poly (dT) X (rA)10 than alpha 2 did.
(11) Our results indicate that provirus expression occurs by two different mechanisms: one provirus acquired a single base pair mutation in the retrovirus tRNA primer binding site, permitting provirus expression; expression of three proviruses was mediated by 5'-flanking DNA sequences.
(12) Cognate sites in genomes that diverged approximately 100 million years ago can be detected by PCR assays based on primer pairs from unique sequences.
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) This reaction involved the synthesis of a short oligoribonucleotide primer which was then extended into a DNA chain.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) B.subtilis phage M2 uses a protein, instead of RNA, as the primer of its DNA replication.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) Primer extension experiments show that in fission yeast transcripts are initiated at the same starting point as in tomato, indicating for the first time that a plant promoter can be correctly recognized in fission yeast.
(19) Genomic clones for the mouse estrogen receptor have been isolated from a cosmid library and used in conjunction with the cDNA clones to study the expression of the receptor in vivo by RNase mapping, primer extension, and Northern blotting.
(20) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
Speaking
Definition:
(p. pr. & vb. n.) of Speak
(a.) Uttering speech; used for conveying speech; as, man is a speaking animal; a speaking tube.
(a.) Seeming to be capable of speech; hence, lifelike; as, a speaking likeness.
Example Sentences:
(1) But when he speaks, the crowds who have come together to make a stand against government corruption and soaring fuel prices cheer wildly.
(2) Whittingdale also defended the right of MPs to use privilege to speak out on public interest matters.
(3) The cause has been innumerable "VIP movements", as journeys undertaken by those considered important enough for all other traffic to be held up, sometimes for hours, are described in South Asian bureaucratic speak.
(4) Many speak about how yoga and surfing complement each other, both involving deep concentration, flexibility and balance.
(5) Speaking to pro-market thinktank Reform, Milburn called for “more competition” and said the shadow health team were making a “fundamental political misjudgment” by attempting to roll back policies he had overseen.
(6) Speaking to a handpicked audience of community representatives, the prime minister said he had not allowed the EU to get its way.
(7) Technically speaking, this modality of brief psychotherapy is based on the nonuse of transferential interpretations, on impeding the regression od the patient, on facilitating a cognitice-affective development of his conflicts and thus obtain an internal object mutation which allows the transformation of the "past" into true history, and the "present" into vital perspectives.
(8) The distribution of cells at the stage of DNA synthesis and mitosis in all the parietal peritoneum speaks of the absence of special proliferation zones.
(9) Again, the boys in care that he abused now speak to us as broken adults.
(10) It’s the same story over and over.” Children’s author Philip Ardagh , who told the room he once worked as an “unprofessional librarian” in Lewisham, said: “Closing down a library is like filing off the end of a swordfish’s nose: pointless.” 'Speak up before there's nothing left': authors rally for National Libraries Day Read more “Today proves that support for public libraries comes from all walks of life and it’s not rocket science to work out why.
(11) Speaking in the BBC's Radio Theatre, Hall will emphasise the need for a better, simpler BBC, as part of efforts to streamline management.
(12) The ability to demonstrate selective augmentation of the functional matrix-associated receptor population, and our recent results showing that gonadotropes are indeed the responsive cells (Singh P, Muldoon TG, unpublished observations) speak to the specificity and relevance of these findings.
(13) Clare Gills, an American journalist and friend of Foley, wrote in 2013: “He is always striving to get to the next place, to get closer to what is really happening, and to understand what moves the people he’s speaking with.
(14) There is a certain degree of swagger, a sudden interruption of panache, as Alan Moore enters the rather sterile Waterstones office where he has agreed to speak to me.
(15) The debate certainly hit upon a larger issue: the tendency for people in positions of social and cultural power to tell the stories of minorities for them, rather than allowing minority communities to speak for themselves.
(16) Speaking to reporters at the Pentagon, People's Liberation Army's chief of the general staff Gen Fang Fenghui also warned that the US must be objective about tensions between China and Vietnam or risk harming relations between Washington and Beijing.
(17) Speaking at The Carbon Show in London today, Philippe Chauvancy, director at climate exchange BlueNext, said that the announcement last week that it is to develop China's first standard for voluntary emission reduction projects alongside the government-backed China Beijing Environmental Exchange, could lay the foundations for a voluntary cap-and-trade scheme.
(18) "There were around 50 attackers, heavily armed in three vehicles, and they were flying the Shebab flag," Maisori added, speaking from the town, where several buildings including hotels, restaurants, banks and government offices were razed to the ground.
(19) Maryam Namazie, an Iranian-born campaigner against religious laws, had been invited to speak to the Warwick Atheists, Secularists and Humanists Society next month.
(20) A doctor the Guardian later speaks to insists it makes no sense.