(1) A Puf- strain of Rhodobacter sphaeroides (PUFB1) was constructed by deleting a portion of the proximal region of the puf operon and inserting a kanamycin resistance gene cartridge.
(2) Chromosome banding patterns are very similar between tissues, but puffing patterns show considerable differences.
(3) The adjacent region, 23B2, is slightly puffed and displays typical RNP particles, some of which may be observed close to band 2-23B1,2.
(4) At the same time the RNA quantity increases by a factor of 2. thermal denaturation profiles of formaldehyde fixed chromosomes show that the Tm of this region in puffed and non puffed state differ by 10 degrees C. Moreover these profiles suggest that a large fraction of histone-bound DNA is destabilized during puffing.
(5) He huffed and puffed, gazed at the heavens at times, and at one point he accused the country’s foremost human rights officer of verballing him.
(6) Habitual smokers of perforation-ventilated cigarettes and of channel-ventilated cigarettes (18 male and 18 female subjects each; nicotine yield 0.1-0.3 mg, 0.2 mg, respectively) were compared with respect to different smoke exposure indicators and puffing behavior.
(7) The boosts for perforation-ventilated cigarettes remained unchanged and were reached with only moderately intensified puffing behavior.
(8) Where there were pictures of powerful women, the images tended to be subversive: the same photograph of a grimacing Theresa May was used to illustrate three different stories about the home secretary, and two of the three pictures of the German chancellor showed Angela Merkel puffing out her cheeks, looking mildly absurd.
(9) People don’t have sex within only one borough – an example of why balkanisation is more expensive than collectivism The immediate anxiety was that elected officials are often not public health experts: you might get a very enlightened council, who understood the needs of the disenfranchised and prioritised them; or you might get a bunch of puffed-up moralists who spent their syphilis budget on a new aqua aerobics provision for the overweight.
(10) Southern blot analysis demonstrated that in PUFB1, the defective copy of the puf operon had replaced, through homologous recombination, the normal chromosomal copy.
(11) Heat a little oil in a pan then cook the dumplings until crisp and puffed, then roll in the cinnamon sugar.
(12) Fluorescence polarization measurements of the lipopholic probe, 1, 6-diphenyl-1,3,5-hexatriene (DPH), in the purified lymphocyte plasmalemma from mice fed a diet high in PUF indicated an increase in mobility.
(13) The pufBA messages, which end in a large intercistronic stem-loop structure, are long-lived processing products of the puf operon transcripts.
(14) The best subset of predictors, especially with respect to puffing parameters, was found to vary considerably across smoking conditions and dependent variables.
(15) The influence of temperature (17 and 31 degrees) on the maternal effect of mutation Puffed (Pu) in Drosophila hybrids has been studied.
(16) Last month I was given unrestricted access to the enormous archive the PCGG has assembled in its years of global detective work: the president’s handwritten diary, frequently puffed with self-regard; the notepaper headed “From the office of the president”, with scribbled sums endlessly totting up his cash; minutes of company meetings with his comments scrawled in the margins; contracts; “side agreements”; records of multiple bank accounts; hundreds of share certificates; private investigators’ reports; and tens of thousands of pages of court judgments.
(17) The polytene chromosome puffing patterns of Drosophila guanche were established and compared with those of Drosophila subobscura.
(18) David Cameron was “pumped up” ; Russell Brand and Ed Miliband exchanged “aint’s” and “innits” and puffed out their chests.
(19) The individual response to smoke might be assessed by an analysis of puffing on a single cigarette.
(20) Neither between-subject consistency nor within-subject reproducibility was improved by this paced puffing procedure, despite apparent topographical control.
(1) Combined SEM and TEM examination of the endothelium of compressed segments revealed "craters" and "balloons", blebs and vacuoles, swollen mitochondria, dilated granular endoplasmic reticulum, and subendothelial edema.
(2) Mild fibrosis, swollen mitochondria, and hyper-contracted and overdistended sarcomeres were typical ultrastructural alterations.
(3) The fungicidal activity of six rabbit neutrophil cationic peptides (NP) against resting (dormant) spores, preincubated (swollen) spores, and hyphae of Aspergillus fumigatus and Rhizopus oryzae was examined.
(4) Precipitates of calcium antimonate were formed almost exclusively in swollen clear pinealocytes, in and along their cell membranes, over their nuclei, in mitochondria, the Golgi apparatus, endoplasmic and integrade reticulums, acervuli, in vesicles surrounding synaptic bars, cytoplasmic matrix, and flocculent extracellular material.
(5) Her chest roentgenogram showed a moderate amount of pleural effusion in the left pleural cavity without infiltration in the lung fields and no evidence of swollen hilar or mediastinal lymphnodes.
(6) Effects of alpha- or beta-D-glucose on the respiration of germinated spores (only germinated spores not including swollen spores and elongated spores) of Bacillus subtilis and B. megaterium were studied.
(7) Central nervous system (CNS) cultured neurons while exposed to different concentrations and pH of L-lactic acid exhibited in general chromatin clumping, vacuolization in the cytoplasm, appearance of lipid bodies, accumulation of polyribosomes, cytoplasmic lucency and swollen and aggregation of mitochondria.
(8) The enzyme changes in response to trauma varied widely; some enzymes controlling tissue respiration showed early and rapid changes, increasing in hyperactive, swollen glial cells and vascular endothelium and decreasing in nerve cells and neuropile.
(9) It's possible that it upsets her to think about the past, or perhaps, these days, she saves her animation for the times when she is holding a microphone and standing in front of a swollen, angry crowd.
(10) In acute inflammation, circumscribed ulcers were often seen; along their margins the epithelial cells were swollen and isolated from the surrounding cells.
(11) A diffusely reddish and swollen vaginal mucosa from a 5 year old caucasian female, which experienced recurrent haemorrhages since the first year of life, proves to be a venous angiosis or varicosis, representing a congenital vascular malformation.
(12) Ultrastructurally, swollen and degenerating nerve processes have been found after lithium treatment suggesting a particular sensitivity of these structures to lithium ion.
(13) Investigations with scanning and transmission electron microscopy demonstrated that the HOS test caused the membrane of the sperm tail to swell and the tail fibers to coil several times within the swollen membrane.
(14) The type I epithelial cells of the lung tissue are swollen and show poor cellular structures.
(15) Within 24 h of injection, axons in this nerve became swollen and fused at points along their length.
(16) After 24 h, the extensor digitorum longus and tibialis anterior muscles appeared grossly swollen (edematous) and discolored.
(17) A 70-year-old man died when his car crashed into a swollen river near Earith, Cambridgeshire.
(18) Maximally swollen mitochondria from intermediate-age ARO rats, contracted more rapidly with antimycin addition than those from PF or AF controls.
(19) Clinical index consisted of the Landsbury index, number of swollen joints and duration of morning stiffness.
(20) The chloride cells were swollen and alterations had occurred in their mitochondria and nuclei.