What's the difference between purpurin and stain?

Purpurin


Definition:

  • (n.) A dyestuff resembling alizarin, found in madder root, and extracted as an orange or red crystalline substance.

Example Sentences:

  • (1) A cDNA for purpurin, a secreted 20,000 dalton neural retina cell adhesion and survival protein, has been sequenced and expressed in mammalian cells.
  • (2) Purpurin was not retained intracellularly and did not bind to TTR coupled to Sepharose.
  • (3) Purpurins, which have strong absorption bands above 650 nm.
  • (4) The 20,000-mol-wt protein, called retinal purpurin (RP), stimulates neural retina cell-substratum adhesion and prolongs the survival of neural retina cells in culture.
  • (5) In 30-day tumor regrowth studies, 70% of animals treated with the metallopurpurin derivative SnET2 were free of tumors while 50% of the animals treated with the free-base purpurin ET2 were free of tumor.
  • (6) In spite of UV-vis and mass spectroscopic similarities, the "purpurin" 7 differs from the "purpurins" 6a,b by the loss of ring A.
  • (7) The synthesis of purpurins from etioporphyrin I and coproporphyrin I proceeds in high yield and with a high degree of regioselectivity.
  • (8) Product formation can be rationalized in terms of relief of steric strain about the periphery of the purpurin macrocycle.
  • (9) Purpurin mRNA is found in both embryonic and adult retina, but not the brain, heart, or liver.
  • (10) In contrast to RBP, purpurin was not retained in vitamin A-deficient HeLa cells.
  • (11) Purpurin and the serum RBP are, however, different molecules, for the serum protein is approximately 3,000 D larger than purpurin and has different silver-staining characteristics.
  • (12) Most interestingly, anti-purpurin and anti-HSPG bound only to one end of adhesive filaments.
  • (13) Purpurin derivatives, a group of synthetic photosensitizers, were tested for their photodynamic activity against transplantable N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide-induced urothelial tumors growing in male Fischer 344 rats.
  • (14) In contrast to RBP, expressed purpurin did not bind to transthyretin (TTR).
  • (15) Purpurins are a class of porphyrin derivative that have been shown to have good in vivo cytotoxicity to N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT) induced rat bladder tumors (AY-27) implanted into Fisher 344 rats.
  • (16) Finally, purpurin supports the survival of dissociated ciliary ganglion cells, indicating that RBPs can act as ciliary neurotrophic factors.
  • (17) A 20,000-D protein called purpurin has recently been isolated from the growth-conditioned medium of cultured embryonic chick neural retina cells (Schubert, D., and M. LaCorbiere, 1985, J.
  • (18) Purpurin binds retinol and may play a major role in retinol transport across the interphotoreceptor cell matrix.
  • (19) Ferritin iron was released by a number of bipyridyl radicals including those derived from diquat and paraquat, the anthracycline radicals of adriamycin, daunorubicin and epirubicin, the semiquinones of anthraquinone-2-sulphonate, 1,5 and 2,6-dihydroxyanthraquinone, 1-hydroxyanthraquinone, purpurin, and plumbagin, and the nitroaromatic radicals of nitrofurantoin and metronidazole.
  • (20) In V79 cells, only HA with 2 hydroxy groups in the 1,3 positions (1,3-DHA, purpurin, emodin) or with a hydroxymethyl sidechain (lucidin and aloe-emodin) were mutagenic.

Stain


Definition:

  • (v. t.) To discolor by the application of foreign matter; to make foul; to spot; as, to stain the hand with dye; armor stained with blood.
  • (v. t.) To color, as wood, glass, paper, cloth, or the like, by processess affecting, chemically or otherwise, the material itself; to tinge with a color or colors combining with, or penetrating, the substance; to dye; as, to stain wood with acids, colored washes, paint rubbed in, etc.; to stain glass.
  • (v. t.) To spot with guilt or infamy; to bring reproach on; to blot; to soil; to tarnish.
  • (v. t.) To cause to seem inferior or soiled by comparison.
  • (v. i.) To give or receive a stain; to grow dim.
  • (n.) A discoloration by foreign matter; a spot; as, a stain on a garment or cloth.
  • (n.) A natural spot of a color different from the gound.
  • (n.) Taint of guilt; tarnish; disgrace; reproach.
  • (n.) Cause of reproach; shame.
  • (n.) A tincture; a tinge.

Example Sentences:

  • (1) The patterns observed were: clusters of granules related to the cell membrane; positive staining localized to portions of the cell membrane, and, less commonly, the whole cell circumference.
  • (2) Within the outflow tract wall, the labelled cells were enmeshed by strands of alcian blue-stained extracellular matrix.
  • (3) The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate.
  • (4) The content of the cavities was not stained by any of the immunocytochemical reactions applied.
  • (5) The dependence of fluorescence polarization of stained nerve fibres on the angle between the fibre axis and electrical vector of exciting light (azimuth characteristics) has been considered.
  • (6) Moreover, in DCVC-treated cells the mitochondria could not be stained with rhodamine-123, indicating severe mitochondrial damage and loss of membrane potential.
  • (7) Immunofluorescent staining for HLA-DR showed dermal positivity in 12 of 13 involved- and 9 of 13 uninvolved-skin biopsy specimens from scleroderma patients, compared with only 1 of 10 controls.
  • (8) From these results it was concluded that FITC-Con A staining method applied to smear specimens is more advantageous in the rapidity and the simplicity for tumor cell diagnosis than section specimen method.
  • (9) Further purification of ZAB by filtration through Sephadex G-100 gave a preparation (ZAB2) which contained the common antigen as shown by the cross-reactivity of anti-ZAB2 rat serum with seven stains of N. gonorrhoeae.
  • (10) It has been found that the epidermal staining pattern for ICAM-1 in each of these diseases in distinctive and different in each disease.
  • (11) After either 5 or 10 days of culture with both cytokines, intense immunofluorescent staining for Ia could be identified on the surface of greater than 80-90% of the viable islet cells.
  • (12) In the second comparison, HSV was isolated from 225 of 1,026 (21.9%) specimens and duplicate human foreskin fibroblast cell wells stained at 24 and 72 h were PAP positive in 241 of 1,026 (23.5%).
  • (13) The epithelium of Brunner's gland stained intensely with Ricinus communis agglutinin-I (RCA-I), succinylated-WGA (S-WGA) and wheat-germ agglutinin (WGA), moderately with Bandeirea simplicifolia agglutinin-I (BS-I), Concanavalia ensiformis agglutinin (Con A) peanut agglutinin (PNA) and Ulex europaeus agglutinin-I (UEA-I) and occasionally with Dolichos biflorus agglutinin (DBA), Lens culinaris agglutinin (LCA) and soybean agglutinin (SBA).
  • (14) Using serial section electron microscopic reconstructions as a reference, we have chosen as our standard procedure a method that maximizes both the preservation of the cytoskeleton and the proportion of cells staining, while minimizing the degree of nonspecific staining.
  • (15) One major band with a molecular weight of 12,000 was detected by autofluorography and coincided with the Coomassie staining band of apocytochrome c from S. cerevisiae.
  • (16) Pitlike surface structures seen in negatively stained whole cells and thin sections were correlated with periodically spaced perforations of the rigid sacculus.
  • (17) In the present study, 125 oesophageal biopsies obtained under direct vision at endoscopy from 22 patients with Barrett's oesophagus were systematically studied using fluorescence and peroxidase antiperoxidase single and double-staining immunocytochemical methods employing highly specific antibodies to localize the following peptide-containing cell types in Barrett's mucosa: gastrin, somatostatin, gastric inhibitory polypeptide, motilin, neurotensin and pancreatic glucagon.
  • (18) The rate of nuclei stained by Pr-122 is different from that of Pr-192 in both growing and quiescent cultures.
  • (19) This light microscopic comparison of viable FDA- and nonviable PI-stained cysts of G. muris demonstrates that 2 types of cysts can be distinguished and implies that structural differences can be used to identify these subpopulations of cysts.
  • (20) Benign and malignant epithelial and soft tissue tumors of the skin were usually negatively stained with MoAb HMSA-2.

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